数字农科院2.0

Comparative analysis of model antigen expression level driven by the microneme protein promoters in Eimeria tenella: HIGH-EXPRESSION LEVEL MICRONEME PROTEIN PROMOTER

文献类型： 外文期刊

作者： Lei Wang;Yanzhen Liao;Jingxia Suo;Lin Liang;Ruiying Liang;Jiabo Ding;Yonglan Yu;Xianyong Liu;Xun Suo;Xinming Tang

作者机构：

关键词： Coccidiosis;Expression level;Microneme protein;Model antigen;Vaccine vector

期刊名称： Poultry Science

ISSN： 0032-5791

年卷期： 2025 年 104 卷 10 期

页码：

收录情况： SCIE（2025版）

摘要： Coccidiosis is the most severe parasitic disease affecting the poultry industry, and live vaccines have played a pivotal role in its prevention and control, making them the most successful parasitic disease vaccines in commercial use. The components of live coccidiosis vaccines, which serve as vaccine vectors for presenting antigens from other pathogens, offer several advantages and provide a promising new option for disease control in modern poultry farming. Enhancing the expression of heterologous antigens within the complex components of Eimeria is a key strategy for optimizing coccidial vectors and improving immune efficacy. Since secreted antigens can stimulate strong immune responses in the host, we conducted a comparative analysis of the transcription levels of the microneme protein, the most important secretory organelle in apicomplexan parasites, across different stages of the life cycle. Three candidate genes with high transcription levels throughout the entire life cycle (excluding the unsporulated oocyst stage) were selected: ETH2_1343100, ETH2_0957500, and ETH2_1219600. We amplified the upstream (∼1500 bp) and downstream (∼1000 bp) regions of these genes to serve as promoters and regulatory sequences for the enhanced yellow fluorescent protein (EYFP) reporter gene expression cassette. This cassette was then incorporated into a dual-expression vector, fused with a selection marker consisting of a mutated prolyl-tRNA synthetase (PRS) gene and mCherry. After transfecting the plasmids into sporozoites and obtaining recombinant Eimeria strains with stable EYFP expression, we analyzed the fluorescence intensity of EYFP using laser confocal microscopy combined with fluorescence intensity analysis. Recombinant Eimeria driven by the histone 4 (EtHis4) promoter was used as a control. The results showed that the promoters of the three microneme protein genes regulated fluorescence expression levels approximately three times higher than the EtHis4 promoter, with the ETH2_1343100 promoter proving to be the most effective. These findings provide new regulatory sequence options for the genetic manipulation of Eimeria and the enhancement of foreign gene expression, accelerating its potential application as a vaccine vector.

分类号：

  • 相关文献

[1]Functional Characterization of 11 Tentative Microneme Proteins in Type I RH Strain of Toxoplasma gondii Using the CRISPR-Cas9 System. Zhi Ya Ma,Xiao Jing Wu,Chuan Li,Jin Gao,Yong Jie Kou,Meng Wang,Xing Quan Zhu,Xiao Nan Zheng. 2024

[2]A Comparative Study on the Population Fitness of Three Strains of Nilaparvata lugens (Hemiptera: Delphacidae) Differ in Eye Color-Related Genes. Liu, Shuhua,Wu, Jincai,Liu, Shuhua,Yang, Baojun,Luo, Ju,Tang, Jian.

[3]Molecular Cloning And Characterization Of L.eucine Aminopeptidase Gene From T aenia Pisiformis. Zhang, SH,Cai, XP,Luo, XN,Wang, S,Guo, AJ,Hou, JL,Wu, R. 2018

[4]Genome-Wide Association Study for Seed Dormancy Using Re-Sequenced Germplasm under Multiple Conditions in Rice. Dandan Chen,Wenli Zou,Mingpei Zhang,Jindong Liu,Liang Chen,Ting Peng,Guoyou Ye. 2023

[5]Cloning, characterization and bioinformatics analysis of the sequences of mir-10a and mir-10b in sheep (hu sheep). Zhibo Wu,Zhenhan Zhai,Mengyao Wang,Hongxiang Ding,Huibin Shi,Zhilong Tian,Xiaohui Zhang,Yuqin Wang,Caihong Wei,Fadi Li. 2021

[6]A flavonol synthase (FLS) gene, GhFLS1, was screened out increasing salt resistance in cotton. Mingge Han,Ruifeng Cui,Yupeng Cui,Junjuan Wang,Shuai Wang,Tiantian Jiang,Hui Huang,Yuqian Lei,Xiaoyu Liu,Cun Rui,Yapeng Fan,Yuexin Zhang,Kesong Ni,Liangqing Sun,Xiugui Chen,Xuke Lu,Delong Wang,Zujun Yin,Chao Chen,Lixue Guo,Lanjie Zhao,Quanjia Chen,Wuwei Ye. 2023

[7]Porcine reproductive and respiratory syndrome virus as a vector: Immunogenicity of green fluorescent protein and porcine circovirus type 2 capsid expressed from dedicated subgenomic RNAs. Du, Yijun,Song, Cheng,Yoo, Dongwan,Pei, Yanlong,Hodgins, Douglas C.,Wu, Jiaqiang,Song, Cheng,Welch, Siao-Kun W.,Calvert, Jay G.,Li, Gang. 2009

[8]Recombinant infectious bursal disease virus expressing Newcastle disease virus (NDV) neutralizing epitope confers partial protection against virulent NDV challenge in chickens. Li, Kai,Gao, Li,Gao, Honglei,Qi, Xiaole,Gao, Yulong,Qin, Liting,Wang, Yongqiang,Wang, Xiaomei.

[9]Towards Innovative Design And Application Of Recombinant Eimeria As A Vaccine Vector. Tang, XM, Liu, XY, Suo, X. 2020

[10]The commercial PRRSV attenuated vaccine can be a potentially effective live trivalent vaccine vector. Yang Li,Yumiao Wang,Xiuxiu Pei,Yongshuai Wu,Shao Chen,Han Weng,Yang Jing,Zhiqian Ma,Zhiwei Li,Zifang Zheng,Yingtong Feng,Lele Xu,Xuyang Guo,Xiao Liu,Jianwu Zhang,Haixue Zheng,Shuqi Xiao. 2025

[11]Advancements, challenges, and future perspectives in developing feline herpesvirus 1 as a vaccine vector. Xinru Luo,Ruiying Liang,Lin Liang,Aoxing Tang,Shaohua Hou,Jiabo Ding,Zibin Li,Xinming Tang. 2024

[12]The complete mitochondrial genome sequence of Eimeria magna (Apicomplexa: Coccidia). Tian, Si-Qin,Wang, Chun-Ren,Zhu, Xing-Quan,Tian, Si-Qin,Liu, Guo-Hua,Zhu, Xing-Quan,Cui, Ping,Fang, Su-Fang,Liu, Guo-Hua,Zhu, Xing-Quan. 2015

[13]Safety evaluation of a triazine compound nitromezuril by assessing bacterial reverse mutation, sperm abnormalities, micronucleus and chromosomal aberration. Fei, Chenzhong,Wang, Xiaoyang,Zhang, Keyu,Zhang, Lifang,Zheng, Wenli,Wang, Mi,Li, Tao,Xiao, Sui,Xue, Feiqun,Wang, Chunmei,Zhang, Jie,Lin, Yang. 2015

[14]Pathogenic effects of the coccidium Eimeria ninakohlyakimovae in goats. Dai, YB,Liu, XY,Liu, M,Tao, JP. 2006

[15]Coccidiosis immunization: Effects of mushroom and herb polysaccharides on immune responses of chickens infected with Eimeria tenella. Guo, FC,Kwakkel, RP,Williams, BA,Suo, X,Li, WK,Verstegen, MWA. 2005

[16]The complete mitochondrial genome sequence of Eimeria mitis (Apicomplexa: Coccidia). Liu, Guo-Hua,Hou, Jie,Song, Hui-Qun,Zhu, Xing-Quan,Liu, Guo-Hua,Zhu, Xing-Quan,Hou, Jie,Weng, Ya-Biao,Li, Shu,Yuan, Zi-Guo,Lin, Rui-Qing,Zhu, Xing-Quan.

[17]Prevalence of Coccidiosis in Domestic Rabbits (Oryctolagus cuniculus) in Northwest China. Qiao, J.,Meng, Q. L.,Chen, C. F.,Wang, J. W.,Wang, W. S.,Zhang, Z. C.,Cai, K. J.,Yang, L. H.,Qiao, J.,Cai, X. P.,Tian, G. F.. 2012

[18]Assessing The Prevalence And Economic S.ignificance Of Coccidiosis Individually A nd In Combination With Concurrent Infections In Pakistani Commercial Poultry Farms. Rashid, M, Akbar, H, Bakhsh, A, Rashid, MI, Hassan, MA, Ullah, R, Hussain, T, Manzoor, S, Yin, H. 2019

[19]Molecular Characterization And Protective Efficacy O.f The Microneme 2 P rotein From Eimeria Tenella. Yan, M, Cui, XX, Zhao, QP, Zhu, SH, Huang, B, Wang, L, Zhao, HZ, Liu, GL, Li, ZH, Han, HY, Dong, H. 2018

[20]Eimeria proteins: order amidst disorder. Joshua Seun Olajide,Zigang Qu,Shunli Yang,Oyeseyi Joshua Oyelade,Jianping Cai. 2022

作者其他论文 更多>>