数字农科院2.0

Cauispirillum nitrogeniifigens gen. nov., sp. nov., a new member of the family Rhodospirillaceae isolated from oil sludge

文献类型： 外文期刊

作者： Jiang Wei Fu;Du Xiang Zheng;Dao Feng Zhang;Yu Meng Wang;Xiao Xia Zhang;Lei Wang

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关键词： Cauispirillum nitrogeniifigens;oil sludge;Rhodospirillaceae

期刊名称： International Journal of Systematic and Evolutionary Microbiology

ISSN： 1466-5026

年卷期： 2026 年 76 卷 1 期

页码：

收录情况： SCIE（2025版）

摘要： The high carbon to nitrogen ratio in oil-contaminated environments limits the efficiency of bioremediation. Therefore, isolating nitrogen-fixing bacteria from oil-contaminated environments will provide functional strain resources for improving bioremediation efficiency. A novel Gram-stain-negative, spiral-shaped and nitrogen-fixing bacterium, designated strain DQ9T, was isolated from the oil sludge of Daqing Oilfield, Daqing City, Heilongjiang Province, China. Cells grew at 18-45 °C (optimum of 40 °C), at pH of 6.5-9.0 (optimum of 7.0) and with 0-2.0% (w/v) NaCl (optimum of 0%). The respiratory quinone was ubiquinone 8 (Q-8). The major cellular fatty acids were summed feature 8 (C18:1 ω7c and/or C18:1 ω6c) (46.8%), summed feature 3 (C16:1ω7c and/or C16:1 ω6c) (14.6%) and C16:0 (17.4%). The polar lipids consisted of phosphatidylglycerol (PG), phosphatidylethanolamine (PE), an unidentified aminophospholipid (UAPL), an unidentified aminolipid (UAL) and two unidentified lipids (UL1-2). Strain DQ9T exhibited the highest 16S rRNA gene sequence similarities with Novispirillum itersonii subsp. itersonii LMG 4337T (94.10%), N. itersonii subsp. nipponicum LMG 7370T (94.10%), Marispirillum indicum B142T (94.04%) and Caenispirillum bisanense K92T (93.44%). The genomic DNA G+C content was 69.8 mol%. Comparing strain DQ9T with closely related type strains, the digital DNA-DNA hybridization values were 18.5-22.0%, the average nucleotide identity values based on blast+ were 67.88-73.76%, the average amino acid identity values were 58-62% and the percentage of conserved proteins values were 45.53-58.80%. In the phylogenetic trees based on 16S rRNA genes and whole-genome sequences, strain DQ9T formed an independent branch within the family Rhodospirillaceae. Based on the results of phylogenetics, morphology, physiology and chemotaxonomy, strain DQ9T represents a novel species of a novel genus in the family Rhodospirillaceae, for which the name Cauispirillum nitrogeniifigens gen. nov., sp. nov. is proposed. The type strain is DQ9T (=KCTC 8951T=CGMCC 1.65428T).

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