数字农科院2.0

Bradyrhizobium tunisiense sp. nov., a novel rhizobial species isolated from Acacia saligna nodules

文献类型： 外文期刊

作者： Jihed Hsouna;Houda Zouagui;Takwa Gritli;Houda Ilahi;Jia Cheng Han;Muhammad Sulman;Walid Ellouze;Xiao Xia Zhang;Maroua Mansouri;Mustapha Missbah El Idrissi;Soufiane Alami;Pierre Emmanuel Courty;Daniel Wipf;Abdelkader Bekki;James T. Tambong;Bacem Mnasri

作者机构：

关键词： Acacia saligna;Bradyrhizobium;phylogenomics;rhizobia;whole genomes

期刊名称： International Journal of Systematic and Evolutionary Microbiology

ISSN： 1466-5026

年卷期： 2025 年 75 卷 6 期

页码：

收录情况： SCIE（2025版）

摘要： Three slow-growing rhizobial strains, designated as 1AS2LT, 1AS20L and 1AS5L, were isolated from nodules of Acacia saligna in Borj Cedria, northern Tunisia. These strains, which belong to the symbiovar cyanophyllae, were characterized using a polyphasic approach. Phylogenetic analysis of the 16S rRNA (rrs) gene placed these strains within the genus Bradyrhizobium, specifically in the superclade associated with Bradyrhizobium japonicum. Further phylogenetic analysis using concatenated sequences of the recA, atpD, glnII and gyrB genes (totalling 1,734 bp) positioned the strains in a distinct lineage, with Bradyrhizobium shewense identified as their closest related species, sharing a sequence identity of 95.2%. The type strain, 1AS2LT, exhibited average nucleotide identity values of 89.10%, 89.08% and 89.00% with the type strains of the closest valid species: Bradyrhizobium frederickii, B. shewense and Bradyrhizobium ottawaense, respectively. Additionally, digital DNA–DNA hybridization values confirmed the novelty of strain 1AS2LT, showing low similarity (38.0%–38.3%) with the type strains of the closest known species. Phylogenomic analyses based on up-to-date bacterial core genes, Type (Strain) Genome Server and the Genome Taxonomy Database (GTDB) pipelines further supported the uniqueness of the 1AS2LT, 1AS20L and 1AS5L strains. The GTDB analysis also robustly clustered two strains (SZCCT0449 and NSD-1) with our strains, suggesting putative members of the proposed novel species. The differentiation of these novel strains from their closest phylogenetic neighbours was also corroborated by phenotypic, physiological and fatty acid content analyses. Based on genomic, phenotypic and biochemical data, we propose the establishment of a novel species, Bradyrhizobium tunisiense sp. nov., with strain 1AS2LT (=LMG 33170T=DSM 114401T) as the type strain.

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