数字农科院2.0

Rice ragged stunt virus Pns10 induces mitochondrial-mediated apoptosis to promote viral infection in Nilaparvata lugens through disrupting the NlNDUFS1-NlPHB2 interaction

文献类型： 外文期刊

作者： Lianshun Zheng;Shuai Fu;Ming Zeng;Liyan Li;Dan Wang;Shibo Gao;Yunge Zhang;Cui Zhang;Shifang Fei;Xuan Ye;Lele Chen;Qianhui Chen;Yaqin Wang;Xueping Zhou;Yan Xie;Boli Hu;Jianxiang Wu

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期刊名称： PLOS Pathogens

ISSN： 1553-7366

年卷期： 2025 年 21 卷 8 期

页码：

收录情况： SCIE（2025版）

摘要： Apoptosis, a programmed cell death process, plays crucial roles in host antiviral response. Although there are many reports on the relationship between cell apoptosis and viral infection, the mechanisms underlying plant arbovirus-induced apoptosis in insect vectors remain largely unclear. Here, we reported that apoptosis promotes rice ragged stunt virus (RRSV) infection in Nilaparvata lugens (brown planthopper), and RRSV-encoded Pns10 protein can induce apoptosis in N. lugens. The Pns10 interacts with N. lugens NADH:ubiquinone oxidoreductase 75 kDa Fe-S protein 1(NlNDUFS1), a core subunit of mitochondrial complex I. Silencing of NlNDUFS1 expression in N. lugens impaired mitochondrial complex I activity, decreasing ATP production and increasing mitochondrial ROS accumulation. This dysregulation triggers apoptosis to promote RRSV infection in N. lugens. Furthermore, RRSV Pns10 disrupts the interaction between NlNDUFS1 and NlProhibitin 2 (NlPHB2) in N. lugens to impair mitochondrial complex I activity, leading to a decrease of ATP production and an increase of mitochondrial ROS accumulation. The excessive accumulation of mitochondrial ROS causes genomic DNA fragmentation and apoptosis. Collectively, the findings presented here illuminate a novel mechanism by which a plant virus manipulates vector mitochondrial apoptosis to benefit viral infection, and offer insights for future transmission-blocking interventions.

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