数字农科院2.0

Establishment of efficient Trichosanthes mottle mosaic virus-derived gene silencing in cucurbit plants

文献类型： 外文期刊

作者： Cheng Chen;Zhu Fang;Min Du;Changkai Yang;Yukui Yang;Xueping Zhou;Xiuling Yang

作者机构：

关键词： Cucurbits;Flower;Trichosanthes mottle mosaic virus;Virus-induced gene silencing

期刊名称： Stress Biology

ISSN： 2731-0450

年卷期： 2025 年 5 卷 1 期

页码：

收录情况： CSCD（2025-2026年度） ; ; ESCI（2025版）

摘要： The Cucurbitaceae family includes a wide range of economically important fruits and vegetables; however, the laborious and highly inefficient genetic transformation efficacy of cucurbits has hindered the exploration of their gene functions. Virus-induced gene silencing (VIGS) technology, employed from the antiviral RNA silencing defense, has emerged as a viable alternative for high-throughput study of plant gene function. In this study, we successfully established a VIGS system utilizing Trichosanthes mottle mosaic virus (TrMMV), a new member of the genus Tobamovirus. We demonstrated the high efficacy and durability of gene silencing mediated by the TrMMV-VIGS vector in Nicotiana benthamiana, as well as in several cucurbit species, including Cucurbita pepo, Cucumis sativus, C. lanatus, and C. melo. The insertion of 90–400 bp fragments into the vector led to effective silencing of the target gene in both C. sativus and C. melo, with a notably higher silencing efficiency observed in C. melo. Furthermore, the TrMMV-VIGS vector induced a pronounced photobleaching phenotype in the flowers of C. melo, underscoring its potential application in functional genomic research concerning floral traits in this particular species. Taken together, the TrMMV-VIGS system developed herein will facilitate rapid and high-throughput identification of gene functions in cucurbit crops.

分类号：

  • 相关文献

[1]A Distinct Tobamovirus Associated With Trichosanthes kirilowii Mottle Mosaic Disease. Cheng Chen,Min Du,Deliang Peng,Wulun Li,Jingfeng Xu,Xiuling Yang,Xueping Zhou. 2022

[2]Effect of Salinity Stress on Germination, Seedling Growth, Mineral Uptake and Chlorophyll Contents of Three Cucurbitaceae Species. Naseer, Muhammad Noman,Rahman, Faiz Ur,Hussain, Zahoor,Khan, Irshad Ahmad,Aslam, Muhammad Muzammal,Aslam, Ali,Waheed, Hasnain,Khan, Asad Ullah,Iqbal, Shahid. 2022

[3]First Report of Tomato Leaf Curl New Delhi Virus Infecting Several Cucurbit Plants in China. Q. S. Gu,L. Y. Yan,L. M. Liu,W. H. Bao,H. Fang,J. H. Xu,J. F. Li,B. S. Kang,H. J. Wu,K. Wang,X. R. Tao,B. Peng. 2023

[4]An AT-hook gene is required for palea formation and floral organ number control in rice. Jin, Yun,Tong, Hongning,Tang, Jinfu,Li, Dayong,Zhao, Xianfeng,Li, Xiaobing,Jiao, Yuling,Chu, Chengcai,Zhu, Lihuang,Jin, Yun,Tong, Hongning,Tang, Jinfu,Li, Dayong,Zhao, Xianfeng,Li, Xiaobing,Jiao, Yuling,Chu, Chengcai,Zhu, Lihuang,Luo, Qiong,Wang, Aiju,Cheng, Zhijun,Wan, Jianmin. 2011

[5]Application of Herbal Plants in Organic Poultry Nutrition and Production. Shahrajabian M.H., Cheng Q., Sun W.. 2022

[6]Genomic identification of cotton SAC genes branded ovule and stress-related key genes in Gossypium hirsutum. Ma Shuya,Liu Le,Shi Huiyun,Gu Yu,Li Yujun,Ghulam Qanmber. 2023

[7]Metabolomic and genome-wide association studies drive genetic dissection and gene mining in tea plant. Xiaohui Jiang,Jingjing Zhao,Dawei Gao,Xiaoliang Zhang,Haiji Qiu,Lin Liu,Wenjiao Zhang,Yujia Ren,Weiwei Wen. 2025

[8]Metabolomic and genome-wide association studies drive genetic dissection and gene mining in tea plant. Xiaohui Jiang,Jingjing Zhao,Dawei Gao,Xiaoliang Zhang,Haiji Qiu,Lin Liu,Wenjiao Zhang,Yujia Ren,Weiwei Wen. 2024

[9]Fine mapping of the tomato yellow leaf curl virus resistance gene Ty-2 on chromosome 11 of tomato. Yang, Xiaohui,Guo, Yanmei,Wang, Xiaoxuan,Du, Yongchen,Yang, Xiaohui,Yang, Xiaohui,Hutton, Samuel F.,Scott, John W.,Caro, Myluska,Rashid, Md Harunur,Visser, Richard G. F.,Bai, Yuling,Szinay, Dora,de Jong, Hans. 2014

[10]The wheat calcium-dependent protein kinase TaCPK7-D positively regulates host resistance to sharp eyespot disease. Wei, Xuening,Shen, Fangdi,Hong, Yantao,Rong, Wei,Du, Lipu,Liu, Xin,Xu, Huijun,Ma, Lingjian,Zhang, Zengyan,Shen, Fangdi,Ma, Lingjian.

[11]Sucrose synthase FaSS1 plays an important role in the regulation of strawberry fruit ripening. Zhao, Cheng,Hua, Li-Na,Liu, Xiao-Feng,Shen, Yuan-Yue,Guo, Jia-Xuan,Li, Yu-Zhong.

[12]The genes involved in the protective effects of phytohormones in response to Verticillium dahliae infection in Gossypium hirsutum. Zhang, Wenwei,Jian, Guiliang,Qi, Fangjun,Si, Ning.

[13]Genome-wide identification of the geranylgeranyl pyrophosphate synthase (GGPS) gene family involved in chlorophyll synthesis in cotton. Wenxiang Feng,Teame Gereziher Mehari,Hui Fang,Meijun Ji,Zijian Qu,Mengxue Jia,Dongmei Wang,Allah Ditta,Muhammad K.R. Khan,Yunying Cao,Jianyong Wu,Baohua Wang. 2023

[14]Genome-Wide Characterization of the SAMS Gene Family in Cotton Unveils the Putative Role of GhSAMS2 in Enhancing Abiotic Stress Tolerance. Joseph Wanjala Kilwake,Muhammad Jawad Umer,Yangyang Wei,Teame Gereziher Mehari,Richard Odongo Magwanga,Yanchao Xu,Yuqing Hou,Yuhong Wang,Margaret Linyerera Shiraku,Joy Nyangasi Kirungu,Xiaoyan Cai,Zhongli Zhou,Renhai Peng,Fang Liu. 2023

[15]Overexpression of ghmpk3 from cotton enhances cold, drought, and salt stress in arabidopsis. Salisu Bello Sadau,Adeel Ahmad,Sani Muhammad Tajo,Sani Ibrahim,Bello Babatunde Kazeem,Hengling Wei,Shuxun Yu. 2021

[16]The L-Type Lectin-like Receptor Kinase Gene TaLecRK-IV.1 Regulates the Plant Height in Wheat. Mamoudou Saidou,Zengyan Zhang. 2022

[17]Characterization of WOX genes revealed drought tolerance, callus induction, and tissue regeneration in Gossypium hirsutum. Muhammad Tajo S.,Pan Z.,He S.,Chen B.,KM Y.,Mahmood T.,Bello Sadau S.,Shahid Iqbal M.,Gereziher T.,Suleiman Abubakar U.,Joseph M.,Sammani T.,Geng X.,Du X.. 2022

[18]Genome-wide association study of maize resistance to Pythium aristosporum stalk rot. Mengwei Hou,Yanyong Cao,Xingrui Zhang,Shulin Zhang,Tengjiao Jia,Jiwei Yang,Shengbo Han,Lifeng Wang,Jingjing Li,Hao Wang,Lili Zhang,Xiaolin Wu,Canxing Duan,Huiyong Li. 2023

[19]Transcriptional landscape of cotton roots in response to salt stress at single-cell resolution. Li, Pengtao,Liu, Qiankun,Wei, Yangyang,Xing, Chaozhu,Xu, Zhongping,Ding, Fang,Liu, Yuling,Lu, Quanwei,Hu, Nan,Wang, Tao,Zhu, Xiangqian,Cheng, Shuang,Li, Zhaoguo,Zhao, Zilin,Li, Yanfang,Han, Jiangping,Cai, Xiaoyan,Zhou, Zhongli,Wang, Kunbo,Zhang, Baohong,Liu, Fang,Jin, Shuangxia,Peng, Renhai. 2024

[20]BrERF109 positively regulates the tolerances of drought and salt stress in Chinese cabbage. Chao Li,Jian Cui,Yongrui Shen,Qi Zeng,Ruixing Zhang,Xue Bai,Qingguo Sun,Xiaowu Wang,Lin Chen,Baohua Li. 2024

作者其他论文 更多>>