数字农科院2.0

Functional analysis of chitinase genes in Lasioderma serricorne reveals their roles in molting and larval-pupal metamorphosis

文献类型： 外文期刊

作者： De Qian Liu;Jia Yu Zhang;Kang Kang Xu;Can Li;Fang Hao Wan;Guy Smagghe;Wen Jia Yang

作者机构：

关键词： Chitin metabolism;Chitinase;Cuticle development;Gene silencing;Lasioderma serricorne;Molting;Pest control;RNAi

期刊名称： Journal of Stored Products Research

ISSN： 0022-474X

年卷期： 2026 年 116 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： The cigarette beetle Lasioderma serricorne is a major storage pest that infests a wide range of commodities, causing significant economic losses. Chitinases (Chts), enzymes responsible for chitin degradation in insect exoskeletons, represent promising molecular targets for RNA interference (RNAi)-based pest control. In this study, nine Cht/Cht-like genes were cloned and characterized from L. serricorne: LsCht1, LsCht3, LsCht5, LsCht6, LsCht7, LsCht8, LsCht10, LsCht11, and LsIDGF. Each encoded protein contained a conserved glycoside hydrolase family 18 (GH18) catalytic domain, and five also possessed chitin-binding domains (CBDs). Phylogenetic analysis classified these genes into 10 established insect Cht groups, demonstrating both evolutionary conservation and lineage-specific diversification. Developmental expression profiling revealed stage-specific peaks for all LsCht genes, except LsCht8, which was predominantly expressed in larval stages, highlighting their roles in molting and metamorphosis. Tissue-specific analysis indicated that these genes were mainly expressed in the brain, gut, epidermis, and Malpighian tubules, suggesting diverse physiological functions. RNAi-mediated silencing of LsCht5, LsCht7, and LsCht10 caused severe molting defects, including epidermal crumpling, melanization, and high larval mortality. Histological (H&E) staining showed that suppression of these genes disrupted epidermal separation and new cuticle formation. Moreover, knockdown of LsCht5, LsCht7, and LsCht10 significantly reduced total chitinase activity and downregulated multiple genes involved in chitin synthesis and degradation. Collectively, these results demonstrate that LsCht5, LsCht7, and LsCht10 are critical regulators of chitin metabolism and larval–pupal metamorphosis in L. serricorne. This study provides the first comprehensive characterization of the Cht gene family in this pest and identifies key molecular targets for the development of precise RNAi-based biocontrol strategies.

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