数字农科院2.0

Sequence analysis and expression of the calmodulin gene, MCaM-3, in mulberry (Morus L.)

文献类型： 外文期刊

作者： Fang, Rongjun;Hu, Dongqing;Zhang, Yinghua;Li, Long;Zhao, Weiguo;Liu, Li;Cheng, Jialin;Fang, Rongjun;Qi, Jinliang;Yang, Yonghua;Hu, Dongqing;Zhang, Yinghua;Zhao, Weiguo;Liu, Li

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关键词： Morus L. MCaM-3 gene;Molecular cloning;Gene expression

期刊名称： GENES & GENOMICS

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年卷期：

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收录情况： SCI

摘要： A full-length cDNA sequence encoding CaM from mulberry, which we designated MCaM-3 (GenBank accession No: GQ303247), was cloned based on mulberry expressed sequence tags(ESTs). Sequence analysis showed that MCaM-3 is 951 base pairs in length, encoding149 amino acids with a predicted molecular weight of 16.85 kD and an isoelectric point of 3.95. Online SMART analysis showed that the MCaM-3 protein has four EFh functional domains, which can bind calcium. The expression level of MCaM-3dX different developmental stages in mulberry leaves and flowers and in different tissues was investigated. The results showed that MCaM-3 transcripts are most abundantly expressed in mature tissues, such as mature female flowers and climax leaves, and the expression level of the mRNA could be increased significantly under low temperature, drought, and salt stress conditions compared to the normal growth environment. This research will help us understand the resistance mechanism of functional genes in mulberry.

分类号： Q

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