数字农科院2.0

Naringin alleviates pneumonia caused by Klebsiella pneumoniae infection by suppressing NLRP3 inflammasome

文献类型： 外文期刊

作者： Xiao Han Jing;Guan Yu Zhao;Gui Bo Wang;Qi Lin Huang;Wen Shu Zou;Li Na Huang;Wei Li;Zheng Ying Qiu;Rui Hua Xin

作者机构：

关键词： Anti-inflammatory mechanism;Klebsiella pneumoniae;Naringin;NLRP3 inflammasome;Protein assembly

期刊名称： Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

ISSN： 1950-6007

年卷期： 2024 年 170 卷

页码：

收录情况： SCIE（2024版）

摘要： Klebsiella pneumoniae (Kpn) is an important pathogen of hospital-acquired pneumonia, which can lead to sepsis and death in severe cases. In this study, we simulated pneumonia induced by Kpn infection in mice to investigate the therapeutic effect of naringin (NAR) on bacterial-induced lung inflammation. Mice infected with Kpn exhibited increases in white blood cells (WBC) and neutrophils in the peripheral blood and pathological severe injury of the lungs. This injury was manifested by increased expression of the inflammatory cytokines interleukin (IL)- 18, IL-1β, tumor necrosis factor-α (TNF-α) and IL-6, and elevated the expression of NLRP3 protein. NAR treatment could decrease the protein expression of NLRP3, alleviate lung inflammation, and reduce lung injury in mice caused by Kpn. Meanwhile, molecular docking results suggest NAR could bind to NLRP3 and Surface Plasmon Resonance (SPR) analyses also confirm this result. In vitro trials, we found that pretreated with NAR not only inhibited nuclear translocation of nuclear factor (NF)-κB protein P65 but also attenuated the protein interaction of NLRP3, caspase-1 and ASC and inhibited the assembly of NLRP3 inflammasome in mice AMs. Additionally, NAR could reduce intracellular potassium (K+) efflux, inhibiting NLRP3 inflammasome activation. These results indicated that NAR could protect against Kpn-induced pneumonia by inhibiting the overactivation of the NLRP3 inflammasome signaling pathway. The results of this study confirm the efficacy of NAR in treating bacterial pneumonia, refine the mechanism of action of NAR, and provide a theoretical basis for the research and development of NAR as an anti-inflammatory adjuvant.

分类号：

  • 相关文献

[1]Naringin’s Alleviation of the Inflammatory Response Caused by Actinobacillus pleuropneumoniae by Downregulating the NF-κB/NLRP3 Signalling Pathway. Huang Q.,Li W.,Jing X.,Liu C.,Ahmad S.,Huang L.,Zhao G.,Li Z.,Qiu Z.,Xin R.. 2024

[2]Assembly of oleosin during efficient extraction: Altering the sequence of defatting solvents. Yu Li,Yuqian Qiao,Yuxuan Zhu,Wangyang Shen,Weiping Jin,Dengfeng Peng,Qingrong Huang. 2025

[3]Palmatine inhibits TRIF-dependent NF-kappa B pathway against inflammation induced by LPS in goat endometrial epithelial cells. Yan, Baoqi,Wang, Dongsheng,Dong, Shuwei,Sang, Mengqi,Yang, Zhiqiang,Zhang, Shidong,Yan, Zuoting,Na, Lidong,Yang, Hongzao,Cheng, Zhangrui.

[4]Naringin attenuates Actinobacillus pleuropneumoniae-induced acute lung injury via MAPK/NF-κB and Keap1/Nrf2/HO-1 pathway. Qi Lin Huang,Li Na Huang,Guan Yu Zhao,Chen Liu,Xiang Yi Pan,Zhao Rong Li,Xiao Han Jing,Zheng Ying Qiu,Rui Hua Xin. 2024

[5]A Novel Ethanolic Two-Phase System Based on Deep Eutectic Solvents and Amphiphilic Copolymer for the Extraction of Neohesperidin and Naringin from the Pomelo Peel. Shanshan Wang,Zongkui Qin,Yicong Wang,Leilei Liu,Zhijian Tan. 2022

[6]Thermal stability, antioxidant activity and bioavailability of pea protein–naringin Pickering emulsion for enhanced delivery applications. Meigui Huang,Mengwei Tian,Chen Tan,Ruifeng Ying,Mehraj Ahmad,Gang Hao,Qiuhong Liao. 2024

[7]Transcriptomic and metabolomic analyses reveal molecular mechanisms of tobacco mosaic virus (TMV) resistance in Nicotiana tabacum L.. Caiyue Li,Qiang Zhang,Xuebo Wang,Zhengwen Liu,Xinyao Zhang,Caihong Jiang,Lirui Cheng,Aiguo Yang,Yangyang Li,Yan Ji,Dan Liu. 2025

[8]The Klebsiella pneumoniae nitrogenase Fe protein gene (nifH) functionally substitutes for the chlL gene in Chlamydomonas reinhardtii. Cheng, Q,Day, A,Dowson-Day, M,Shen, GF,Dixon, R. 2005

[9]Complete genome sequence of endophytic nitrogen-fixing Klebsiella variicola strain DX120E. Lin, Li,Li, Yangrui,Yang, Litao,Lin, Li,Li, Yangrui,Yang, Litao,Lin, Li,Li, Yangrui,Yang, Litao,Lin, Li,Li, Yangrui,Yang, Litao,Wei, Chunyan,Li, Yangrui,Yang, Litao,Chen, Mingyue,Wang, Hongcheng,Li, Yuanyuan,An, Qianli,Yang, Litao. 2015

[10]Molecular Epidemiology and Antimicrobial Resistance of Outbreaks of Klebsiella pneumoniae Clinical Mastitis in Chinese Dairy Farms. Fu, Shaodong,Wen, Chen,Wang, Zhenglei,Qiu, Yawei,Zhang, Yihao,Zuo, Jiakun,Xu, Yuanyuan,Han, Xiangan,Luo, Zhenhua,Chen, Wei,Miao, Jinfeng. 2022

[11]A Small KPC-2-Producing Plasmid in Klebsiella pneumoniae: Implications for Diversified Vehicles of Carbapenem Resistance. Chen, Qiwei,Liu, Lizhang,Hu, Xiaofang,Jia, Xu,Gong, Xiaowei,Feng, Youjun,Huang, Man. 2022

[12]Co-infections of Klebsiella pneumoniae and Elizabethkingia miricola in black-spotted frogs (Pelophylax nigromaculatus). Shurui Li,Xiaoni Wang,Yulan Lu,Jiahuan Wang,Denghang Yu,Zhigang Zhou,Jin Wei,Lihe Liu,Jun Liu,Fuguo Liu,Yingbing Su,Hongsen Xu. 2023

[13]Construction and application of the conditionally essential gene knockdown library in Klebsiella pneumoniae to screen potential antimicrobial targets and virulence genes via Mobile-CRISPRi-seq. Qing Zhu,Qiang Lin,Yushan Jiang,Shuyan Chen,Junxuan Tian,Shijin Yang,Yuanchun Li,Mengjun Li,Yuelin Wang,Chenguang Shen,Songdong Meng,Liang Yang,Youjun Feng,Jiuxin Qu. 2023

[14]Isobavachalcone enhances sensitivity of colistin-resistant Klebsiella pneumoniae: In vitro and in vivo proof-of-concept studies. Xiang Geng,Yuxi Li,Ruochen Hao,Chunyan Xu,Zhun Li,Yajun Yang,Xiwang Liu,Jianyong Li,Wanxia Pu. 2024

[15]Whole-genome sequencing of multidrug-resistant Klebsiella pneumoniae with capsular serotype K2 isolates from mink in China. Na Sun,Yong Yang,Guisheng Wang,Li Guo,Liming Liu,Zhihao San,Cuiqing Zhao,Lifeng Zhao,Mingwei Tong,Yuening Cheng,Qiang Chen. 2024

[16]Draft genome sequence analysis of a mcr-1-producing Klebsiella pneumoniae ST661 isolated from a mink in China. Na Sun,Yilin Luo,Yaru Zhao,Guisheng Wang,Li Guo,Liming Liu,Zhihao San,Cuiqing Zhao,Yuening Cheng,Qiang Chen. 2024

[17]Efficacy of a new K3-specific bacteriophage for controlling Klebsiella pneumoniae in milk and its potential to disrupt biofilm formation. Pei Li,Yu Zhou,Genglin Guo,Xiayan Qin,Zimeng Hu,Min Li,Zhongming Tan,Yuqing Liu,Xiangan Han,Jiale Ma,Hong Du,Wei Zhang. 2024

[18]Molecular characterization of Klebsiella pneumoniae in clinical bovine mastitis in 14 provinces in China. Wusiman, Maierhaba,Zuo, Jiakun,Yu, Yong,Lv, Zhaoyang,Wang, Mengdi,Nie, Lianhua,Zhang, Xiuping,Wu, Jing,Wu, Zihao,Jiang, Wei,Pan, Zihao,Zhang, Wei,Yin, Huifang,Huang, Cuiqin,Chen, Zhaoguo,Miao, Jinfeng,Chen, Wei,Han, Xiangan. 2025

[19]Antibiotic Resistance of Escherichia coli and Klebsiella pneumoniae Isolated from Wild Raccoon Dogs in Shanghai, China. Xiong, Xinhuo,Chen, Chen,Tao, Jin,Wei, Min,Wang, Xiaoyang,Wang, Chunmei,Ye, Wenchong,Zhou, Wen,Liu, Guoping,Zhang, Keyu. 2025

[20]Outer Membrane Vesicles Attenuate Klebsiella pneumoniae Infection Injury by Affecting Macrophage Polarisation and Helper T Cell Differentiation. Wei Fan,Wei Wang,Lin Kong,Shifan Chen,Xinyu Zhang,Yilu Zhai,Bo Zhang,Yan Wang,Dazhuo Zhao,Xiukai Tang,Jiaqi Fu,Fuliang Sun. 2025

作者其他论文 更多>>