数字农科院2.0

Characterization of the wall-associated kinase (WAK) gene family in Gossypium barbadense reveals the positive role of GbWAK5 in salt tolerance

文献类型： 外文期刊

作者： Zhiqiang Zhang;Wenyu Ma;Haijuan Wang;Zhongying Ren;Yangai Liu;Kunlun He;Fei Zhang;Wuwei Ye;Wenqi Huo;Wei Li;Xiongfeng Ma;Daigang Yang

作者机构：

关键词： Na+/K+;Salt stress;Sea island cotton;VIGS;Wall-associated kinases

期刊名称： Plant Cell Reports

ISSN： 0721-7714

年卷期： 2025 年 44 卷 1 期

页码：

收录情况： SCIE（2025版）

摘要： Key message: We characterized the WAK gene family in Gossypium barbadense and revealed the potential function of GbWAK5 in regulating salt tolerance by modulating ion homeostasis. Abstract: Soil salinization is one of the main factors restricting cotton production. Although the role of the wall-associated kinases (WAKs) in plants has been extensively studied, its response to salt stress in sea-island cotton (Gossypium barbadense L.) has not been reported. Here, we conducted a whole-genome analysis of the WAK gene family in G. barbadense, identifying a total of 70 GbWAK genes, which were classified into five clades. Segmental and tandem duplication events have contributed to the expansion of the GbWAK gene family. A large number of cis-acting elements were predicted in the GbWAK promoter region. Through RNA sequencing, 37 GbWAKs that potentially play a role in cotton's response to salt stress were screened out, among which 10 genes with sustained up-regulated expression were confirmed by quantitative real-time PCR (qRT-PCR). GbWAK5, a member of Clade II, was significantly up-regulated following NaCl treatment and exhibited a typical WAK structure. Subcellular localization indicated that GbWAK5 is localized on the plasma membrane. Virus-induced gene silencing (VIGS) experiments revealed that the knockdown of GbWAK5 resulted in more severe dehydration and wilting in plants compared to the control under NaCl treatment. RNA-seq analysis revealed that several ion transport-related genes were down-regulated in TRV:GbWAK5 plants under salt stress, while TRV:GbWAK5 plants accumulated more Na+ and exhibited a higher Na+/K+ ratio compared to TRV:00 plants. These results offer a comprehensive analysis of the G. barbadense WAK gene family for the first time, and conclude that GbWAK5 is a promising gene for improving cotton's resistance to salt stress.

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