数字农科院2.0

Genomic Insights into ARR Genes: Key Role in Cotton Leaf Abscission Formation

文献类型： 外文期刊

作者： Hongyan Shi;Zhenyu Wang;Yuzhi Zhang;Gongye Cheng;Peijun Huang;Li Yang;Songjuan Tan;Xiaoyu Cao;Xiaoyu Pei;Yu Liang;Yu Gao;Xiang Ren;Quanjia Chen;Xiongfeng Ma

作者机构：

关键词： ARR genes family;Gossypium hirsutum;leaf abscission;VIGS

期刊名称： International Journal of Molecular Sciences

ISSN： 1661-6596

年卷期： 2025 年 26 卷 15 期

页码：

收录情况： SCIE（2025版）

摘要： The cytokinin response regulator (ARR) gene is essential for cytokinin signal transduction, which plays a crucial role in plant growth and development. However, the functional mechanism of ARR genes in cotton leaf abscission remains incompletely understood. In this study, a total of 86 ARR genes were identified within the genome of Gossypium hirsutum. These genes were categorized into four distinct groups based on their phylogenetic characteristics, supported by analyses of gene structures and conserved protein motifs. The GhARR genes exhibited an uneven distribution across 25 chromosomes, with three pairs of tandem duplication events observed. Both segmental and tandem duplication events significantly contributed to the expansion of the ARR gene family. Furthermore, numerous putative cis-elements were identified in the promoter regions, with hormone and stress-related elements being common among all 86 GhARRs. Transcriptome expression profiling screening results demonstrated that GhARRs may play a mediating role in cotton’s response to TDZ (thidiazuron). The functional validation of GhARR16, GhARR43, and GhARR85 using virus-induced gene silencing (VIGS) technology demonstrated that the silencing of these genes led to pronounced leaf wilting and chlorosis in plants, accompanied by a substantial decrease in petiole fracture force. Overall, our study represents a comprehensive analysis of the G. hirsutum ARR gene family, revealing their potential roles in leaf abscission regulation.

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