数字农科院2.0

Engineering the high-spin 3d orbital in Co3O4 via compressive strain to enhance immunochromatographic assay

文献类型： 外文期刊

作者： Cui Zhang;Zhaoli Liu;Xuan Ye;Weijia Mao;Jie Dong;Bin Li;Xueping Zhou;Jinyan Luo;Jianxiang Wu

作者机构：

关键词： High spin-states;Lateral flow immunoassay;Peroxidase-like nanozyme;Strain engineering;Xanthomonas oryzae pv. oryzae

期刊名称： Chemical Engineering Journal

ISSN： 1385-8947

年卷期： 2025 年 523 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Enhancing catalytic activity of peroxidase-like nanozymes is essential for developing ultrasensitive lateral flow immunoassay (LFIA). The peroxidase-like catalytic process involves spin-dependent electron transfer during radical formation, and boosting single electron transfer (SET) efficiency is therefore critical to enhance catalytic performance. Here, we fabricated a strained-Co3O4 exhibiting significant atomic compressive strain (εxx = −3.34 %, εyy = −6.52 %). The atomic-level displacement direction and distance information determined by atomic displacement analysis (ADA) offer detailed information for density functional theory computational modeling. We discovered that the strain engineering elevated crystal field splitting energy, which promotes the transition of Co3O4 from low-spin states (t2g6eg0) to high-spin states (t2g4eg2), resulting in efficient SET performance. Spin-state modulation dramatically enhances the peroxidase-like catalytic efficiency, resulting in 28.0-fold and 11.8-fold lower Michaelis constants (Km) for TMB and H2O2, respectively, than those of the pristine-Co3O4. In addition, the strained-Co3O4 was successfully employed in the development of LFIA for Xanthomonas oryzae pv. oryzae (Xoo) monitoring, and the LFIA achieved a detection sensitivity of 5 × 102 CFU mL−1 that was 20-fold higher than Au nanoparticles-based LFIA and was 2–3 orders of magnitude higher than commercial LFIAs. Our findings established a correlation between strain–spin orbit–peroxidase-like activity, offering a promising strategy for the rational design of nanozymes.

分类号：

  • 相关文献

[1]Development and validation of a lateral flow immunoassay using colloidal gold for the identification of serotype-specific foot-and-mouth disease virus O, A and Asia 1. Jiang, Tao,Liang, Zhong,Ren, Weiwei,Chen, Juan,Zhi, Xiaoying,Qi, Guangyu,Yang, Yamin,Liu, Zaixing,Liu, Xiangtao,Cai, Xuepeng,Ren, Weiwei,Zhi, Xiaoying,Qi, Guangyu. 2011

[2]Self-Assembly Multivalent Fluorescence-Nanobody Coupled Multifunctional Nanomaterial with Colorimetric Fluorescence and Photothermal to Enhance Immunochromatographic Assay. Li, Zhiqiang,Zhang, Wen,Zhang, Qi,Li, Peiwu,Tang, Xiaoqian. 2023

[3]Highly efficient detection of deoxynivalenol and zearalenone in the aqueous environment based on nanoenzyme-mediated lateral flow immunoassay combined with smartphone. Weibin Li,Zedong Wang,Xinwei Wang,Li Cui,Wenyuan Huang,Zhaoyong Zhu,Zhenjiang Liu. 2023

[4]A semi-quantitative multi-range lateral flow immunoassay for amantadine residues in livestock and poultry products. Xiangxue Ning,Jing Qiu. 2022

[5]A versatile nanozyme integrated colorimetric and photothermal lateral flow immunoassay for highly sensitive and reliable Aspergillus flavus detection. Meijuan Liang,Xinfa Cai,Yaoyao Gao,Honglin Yan,Jiayun Fu,Xiaoqian Tang,Qi Zhang,Peiwu Li. 2022

[6]On-site rapid detection of multiple pesticide residues in tea leaves by lateral flow immunoassay. Gao, Junxia,Zhang, Tianyi,Fang, Yihua,Zhao, Ying,Yang, Mei,Zhao, Li,Li, Ye,Huang, Jun,Zhu, Guonian,Guo, Yirong. 2024

[7]NIR-II fluorescent Ag2Se polystyrene beads in a lateral flow immunoassay to detect biomarkers for breast cancer. Kuhan Deng,Zi Li Yu,Xiaofeng Hu,Jing Liu,Xuechuan Hong,Gong Ga Lan Zi,Zhaowei Zhang,Zhi Quan Tian. 2023

[8]Half-antibody spur one-to-one recognition mechanism can function with chemical-staining technology for next-generation lateral flow immunoassay. Xu, Jingke,Zhou, Jing,Liu, Kai,Bu, Tong,Dou, Leina,Shu, Rui,Liu, Sijie,Wang, Shaochi,Yin, Xuechi,Zhang, Daohong,Zhang, Ruiling,Cheng, Jie,Wang, Jianlong. 2024

[9]Multi-chromatic and multi-component lateral flow immunoassay for simultaneous detection of CP4 EPSPS, Bt-Cry1Ab, Bt-Cry1Ac, and PAT/bar proteins in genetically modified crops. Yang, Yao,Zhang, Zini,Wang, Zhi,Pan, Ruxin,Wu, Huimin,Zhai, Shanshan,Wu, Gang,Fu, Wei,Gao, Hongfei. 2025

[10]Multi-mode probe based on dual colorimetric and photothermal later flow immunoassay for the ultrasensitive determination of benzo[a]pyrene in vegetable oils. Yuting Yuan,Xiaoqian Tang,Liangxiao Zhang,Qi Zhang,Fei Ma,Peiwu Li. 2025

[11]Sensitive and portable intelligent detection platform construction and dietary risk assessment of procymidone in Chinese leek, cowpea and celery. Zhang, Binbin,Li, Shi,Zhang, Wentao,Cheng, Youpu,Liu, Zhenjiang,Zhang, Ning,Xu, Jun,Wu, Xiaohu,Dong, Fengshou,Zheng, Yongquan,Pan, Xinglu. 2025

[12]Nanobody-based dual-mode sensing platform for highly sensitive detection of aflatoxin B1. Qinkai Yang,Huilin Jia,Guangsheng Ge,Mengnan Wang,Shurong Li,Xuan Liu,Xueyi Fan,Kai Zheng,Xiaoqian Tang,Qi Zhang,Jianlong Wang,Ran Chen,Yanru Wang. 2025

[13]Optimized Electrofusion for Efficient Generation of Monoclonal Antibodies against Chlorpyrifos and Its Application in Lateral Flow Immunoassay. You, Tianyang,Wang, Zhijia,Yang, Shiyuan,Wang, Jitao,Ding, Yuan,Zheng, Lufei,Hua, Xiude. 2025

[14]Development of a reliable and convenient lateral flow immunoassay for dihydromyricetin detection in Ampelopsis grossedentata samples. Cui, Xueyan,Cao, Zhen,Yu, Hailong,Li, Hui,Ying, Ying,Luo, Ting,Wang, Jing. 2025

[15]Rpon2-And Flia-Regulated Flitx Is Indispensible F.or Flagellar Motility And V irulence In Xanthomonas Oryzae Pv. Oryzae. Yu, Chao,Yang, Fenghuan,Tian, Fang,He, Chenyang,Chen, Huamin. 2017

[16]Novel Insights into Rice Innate Immunity Against Bacterial and Fungal Pathogens. Wang, Guo-Liang,Liu, Jinling,Liu, Jinling,Triplett, Lindsay,Leach, Jan E.,Wang, Guo-Liang. 2014

[17]Comparative transcriptome profiling of a rice line carrying Xa39 and its parents triggered by Xanthomonas oryzae pv. oryzae provides novel insights into the broad-spectrum hypersensitive response. Zhang, Fan,Huang, Li-Yu,Zhang, Fan,Zhuo, Da-Long,Li, Zhi-kang,Zhou, Yong-Li,Huang, Li-Yu,Ali, Jauhar,Cruz, Casiana Vera,Du, Zheng-Lin. 2015

[18]Characteristic Expression Analysis of Five WRKY Transcriptional Factors in Rice Leaf Growth and Disease Resistance Reaction. Miao Liu-Yang,Li Li-Yun,Liu Zhao,Liu Yu-Meng,Liu Guo-Zhen,Jiang Guang-Huai,Yang Feng-Huan,He Chen-Yang. 2013

[19]XA23 Is an Executor R Protein and Confers Broad-Spectrum Disease Resistance in Rice. Wang, Chunlian,Zhang, Xiaoping,Fan, Yinglun,Gao, Ying,Zheng, Chongke,Qin, Tengfei,Li, Yanqiang,Che, Jinying,Zhang, Mingwei,Zhao, Kaijun,Zhu, Qinlong,Liu, Yaoguang,Yang, Bing. 2015

[20]Overlap between Signaling Pathways Responsive to Xanthomonas oryzae pv. oryzae Infection and Drought Stress in Rice Introgression Line Revealed by RNA-Seq. Zhang, Fan,Zhang, Fan,Huang, Liyu,Xu, Jianlong,Zhou, Yongli,Li, Zhikang,Xu, Jianlong,Zhou, Yongli,Li, Zhikang,Huang, Liyu,Cruz, Casiana Vera,Ali, Jauhar,Zhang, Fan.

作者其他论文 更多>>