数字农科院2.0

Immunogenic Responses Elicited by a Pool of Recombinant Lactiplantibacillus plantarum NC8 Strains Surface-Displaying Diverse African Swine Fever Antigens Administered via Different Immunization Routes in a Mouse Model

文献类型： 外文期刊

作者： Assad Moon;Hongxia Wu;Tao Wang;Lian Feng Li;Yongfeng Li;Zhiqiang Xu;Jia Li;Yanjin Wang;Jingshan Huang;Tianqi Gao;Yuan Sun;Hua Ji Qiu

作者机构：

关键词： African swine fever;immunological evaluation;lactic acid bacteria;Lactiplantibacillus plantarum;surface display

期刊名称： Vaccines

ISSN： 2076-393X

年卷期： 2025 年 13 卷 9 期

页码：

收录情况： SCIE（2025版）

摘要： Background: African swine fever (ASF) is a highly contagious and often deadly disease that poses a major threat to swine production worldwide. The lack of a commercially available vaccine underscores the critical need for innovative immunization strategies to combat ASF. Methods: Six ASFV antigenic proteins (K78R, A104R, E120R, E183L, D117L, and H171R) were fused with the Lactiplantibacillus plantarum WCFS1 surface anchor LP3065 (LPxTG motif) to generate recombinant Lactiplantibacillus plantarum NC8 (rNC8) strains. The surface expression was confirmed using immunofluorescence and Western blotting assays. Additionally, the dendritic cell-targeting peptides (DCpep) were co-expressed with each antigen protein. Mice were immunized at a dosage of 109 colony-forming units (CFU) per strain per mouse via intragastric (I.G.), intranasal (I.N.), and intravenous (I.V.) routes. The bacterial mixture was heat-inactivated by boiling for 15 min to destroy viable cells while preserving antigenic structures. I.V. administration caused no hypersensitivity, confirming the method’s safety and effectiveness. Results: Following I.G. administration, rNC8-E120R, rNC8-E183L, rNC8-K78R, and rNC8-A104R induced significant levels of secretory immunoglobulin A (sIgA) in fecal samples, whereas rNC8-H171R and rNC8-D117L failed to induce a comparable response. Meanwhile, rNC8-D117L, rNC8-K78R, and rNC8-A104R also elicited significant levels of sIgA in bronchoalveolar lavage fluid (BALF). Following I.N. immunization, rNC8-E120R, rNC8-K78R, and rNC8-A104R significantly increased sIgA levels in both fecal and BALF immunization. In contrast, I.V. immunization with heat-inactivated rNC8-K78R and rNC8-A104R induced robust serum IgG titers, whereas the remaining antigens elicited minimal or insignificant responses. Flow cytometry analysis revealed expanded CD3+CD4+ T cells in mice immunized via the I.N. and I.G. and CD3+CD4+ T cells only in those immunized via the I.N. route. Th1 responses were also significant in the sera of mice immunized via the I.G. and I.N. routes. Conclusions: The rNC8 multiple-antigen cocktail elicited strong systemic and mucosal immune responses, providing a solid foundation for the development of a probiotic-based vaccine against ASF.

分类号：

  • 相关文献

[1]A Pool of Bacterium-like Particles Displaying African Swine Fever Virus Antigens Induces Both Humoral and Cellular Immune Responses in Pigs. Jingshan Huang,Hongxia Wu,Tianqi Gao,Huanjie Zhai,Assad Moon,Xin Song,Shuwen Li,Zhanhao Lu,Jing Lan,Dailang Zhong,Xinyu Zhang,Hua Ji Qiu,Yongfeng Li,Yuan Sun. 2025

[2]Immune Responses Induced by a Recombinant Lactiplantibacillus plantarum Surface-Displaying the gD Protein of Pseudorabies Virus. Assad Moon,Jingshan Huang,Xin Song,Tao Wang,Yanjin Wang,Yongfeng Li,Yuan Sun,Hongxia Wu,Huaji Qiu. 2024

[3]Construction of a cell-surface display system based on the N-terminal domain of ice nucleation protein and its application in identification of mycoplasma adhesion proteins. Bao, S.,Yu, S.,Zhang, F.,Sun, Y.,Tan, L.,Duan, Y.,Qiu, X.,Ding, C.,Bao, S.,Guo, X.,Lu, F..

[4]Surface display of Nocardia seriolae HRP1 on Bacillus subtilis and its application as live vaccine for largemouth bass. Zhang, Fengli,Li, Ji-Tong,Zhang, Tengyuan,Wang, Xiao,Yao, Yuanyuan,Zhou, Zhigang. 2025

[5]Lactiplantibacillus plantarum AR113 Exhibit Accelerated Liver Regeneration by Regulating Gut Microbiota and Plasma Glycerophospholipid. Chunliang Xie,Zhoumei Zhang,Manyi Yang,Cha Cao,Yingjun Zhou,Zuohua Zhu,Wenbing Gong,Chao Xu,Li Yan,Zhenxiu Hu,Lianzhong Ai,Yuande Peng. 2022

[6]Spatial and Temporal Persistence of Fluorescent Lactiplantibacillus plantarum RS-09 in Intestinal Tract. Zhao X.,Zhao C.,Yang L.,Jiang L.,Zhang J.,Yu X.,Chen G.,Zhu H.,Tang W.,Li Y.,Wei M.,Zhang X.,Jia H.. 2022

[7]Lactiplantibacillus plantarum: A comprehensive review of its antifungal and anti-mycotoxic effects. Qianqian Li,Xiangquan Zeng,Hailong Fu,Xiaomei Wang,Xiaojun Guo,Meng Wang. 2023

[8]Co-fermentation of Lactiplantibacillus plantarum and Saccharomyces cerevisiae enhances the flavor characteristic of mature coconut water: Insights from volatile and non-volatile profiles. Wen Xu,Qianqian Li,Zeming Song,Xiaoping Hu,Kun Cai,Lin Zhang,Xue Lin,Sixin Liu,Congfa Li,Qingyang Xu. 2025

[9]Novel strategy to understand the bacteria-enzyme synergy action regulates the ensiling performance of wheat straw silage by multi-omics analysis. Du, Shuai,Xu, Lijun,Jiang, Chao,Xiao, Yanzi. 2025

[10]Fermentation quality and chemical composition of shrub silage treated with lactic acid bacteria inoculants and cellulase additives. Yu, Zhu,Zhao, Shufen,Cai, Yimin,Sun, Qizhong,Gao, Fengqin,Tao, Ya. 2012

[11]Antioxidant activity of Lactobacillus plantarum strains isolated from traditional Chinese fermented foods. Yang, Zhennai,Li, Shengyu,Zhao, Yujuan,Zhang, Li,Zhang, Xue,Huang, Li,Li, Da,Niu, Chunhua,Yang, Zhennai,Zhang, Li,Yang, Zhennai,Wang, Qiang.

[12]Class IIa Bacteriocins: Diversity and New Developments. Wang, Yunfeng,Cui, Hongyu,Cui, Yanhua,Zhang, Chao,Zhang, Lanwei,Ding, Zhongqing,Shi, John,Qu, Xiaojun. 2012

[13]Lactic acid bacteria as mucosal delivery vehicles: a realistic therapeutic option. Wang, Miao,Gao, Zeqian,Zhang, Yongguang,Pan, Li,Zhang, Yongguang,Pan, Li.

[14]Antibacterial And Antibiofilm Activity Of L.actic Acid Bacteria Isolated F rom Traditional Artisanal Milk Cheese From Northeast China Against Enteropathogenic Bacteria. Cui, XY, Shi, YJ, Gu, SS, Yan, X, Chen, HY, Ge, JW. 2018

[15]Probiotic assessment and antioxidant characterization of Lactobacillus plantarum GXL94 isolated from fermented chili. Yingjun Zhou,Wenbing Gong,Chao Xu,Zuohua Zhu,Yuande Peng,Chunliang Xie. 2022

[16]Evaluating the fermentation characteristics, bacterial community, and predicted functional profiles of native grass ensiled with different additives. Du S.,You S.,Jiang X.,Li Y.,Wang R.,Ge G.,Jia Y.. 2022

[17]Highly efficient reduction of ammonia emissions from livestock waste by the synergy of novel manure acidification and inhibition of ureolytic bacteria. Liu J.,Li X.,Xu Y.,Wu Y.,Wang R.,Zhang X.,Hou Y.,Qu H.,Wang L.,He M.,Kupczok A.,He J.. 2023

[18]Antimicrobial, Anti-Adhesive And Anti-Biofilm Potential O.f Biosurfactants Isolated From P ediococcus Acidilactici And Lactobacillus Plantarum Against Staphylococcus Aureus Cmcc26003. Yan, X, Gu, SS, Cui, XY, Shi, YJ, Wen, SS, Chen, HY, Ge, JW. 2019

[19]Mechanism study on enhanced emulsifying properties of phosvitin and calcium-binding capacity of its phosphopeptides by lactic acid bacteria fermentation. Liangjie Tian,Dandan Xiong,Jie Jia,Xuebo Liu,Ya Zhang,Xiang Duan. 2022

[20]Effect of lactic acid bacteria and yeast supplementation on anti-nutritional factors and chemical composition of fermented total mixed ration containing cottonseed meal or rapeseed meal. Yusuf, Hassan Ali,Piao, Minyu,Ma, Tao,Huo, Ruiying,Tu, Yan. 2022

作者其他论文 更多>>