数字农科院2.0

Functional analysis of Ophraella communa Lesage OcomOBP11 in recognition of Ambrosia artemisiifolia L. volatiles

文献类型： 外文期刊

作者： Yang Yue;Chao Ma;Yan Zhang;Wei hua Ma;Jing jing Wang;Zhen ya Tian;Guang mei Chen;Ru wen Li;Jin hua Li;Jing fang Yang;Zhong shi Zhou

作者机构：

关键词： Behavioral responses;EAG;Fluorescence binding assay;Molecular docking;Odorant-binding proteins;Ophraella communa Lesage;RNAi

期刊名称： Pesticide Biochemistry and Physiology

ISSN： 0048-3575

年卷期： 2025 年 210 卷

页码：

收录情况： SCIE（2025版）

摘要： Odorant binding proteins (OBPs) play a critical role in insect survival by facilitating the detection of environmental odors. This study identified and characterized OcomOBP11 in the leaf beetle Ophraella communa Lesage. Sequence and phylogenetic analyses demonstrated that OcomOBP11 belongs to the Minus-C OBP family. Tissue-specific expression analysis revealed that OcomOBP11 showed the highest relative expression in the testis. Functional assays demonstrated that OcomOBP11 has strong binding affinity to 24 of the 26 volatiles from Ambrosia artemisiifolia L., a primary host plant. Molecular docking showed that OcomOBP11 formed hydrophobic interactions with β-caryophyllene, α-pinene, camphene, octanal, and nonanal and hydrogen bonds with octanal and nonanal. RNAi-mediated knockdown of OcomOBP11 significantly reduced the electrophysiological (EAG) response and behavioral preference of O. communa for these ligands. These findings suggest that OcomOBP11 plays an important role in host plant localization, thus providing guidance for future biological invasion control strategies.

分类号：

  • 相关文献

[1]Characterization and Functional Analysis of OcomOBP7 in Ophraella communa Lesage. Yang Yue,Chao Ma,Yan Zhang,Hong Song Chen,Jian Ying Guo,Ting Hui Liu,Zhong Shi Zhou. 2023

[2]Electroantennographic and Behavioral Responses of the Melon fly, Zeugodacus cucurbitae (Coquillett), to Volatile Compounds of Ridge Gourd, Luffa acutangular L.. Wang, Jing jing,Ma, Chao,Tian, Zhen ya,Zhou, Yong ping,Yang, Jin fang,Gao, Xuyuan,Chen, Hong song,Ma, Wei hua,Zhou, Zhong shi. 2024

[3]The larvae of Phyllotreta striolata share the same olfactory cues for locating Brassicaceae plant with conspecific adults. Xiao, Yong,Sun, Liang,Wu, Yuhong,Wang, Qian,Zhang, Yongjun,Jing, Xiangfeng,Li, Zhenyu. 2023

[4]Expression Profiles and Functional Characterization of Chemosensory Protein 15 (HhalCSP15) in the Brown Marmorated Stink Bug Halyomorpha halys. Zehua Wang,Fan Yang,Ang Sun,Shuang Shan,Yongjun Zhang,Shanning Wang. 2021

[5]Characterization of the chemosensory protein EforCSP3 and its potential involvement in host location by Encarsia formosa. WANG K.,HE Y.-Y.,ZHANG Y.-J.,GUO Z.-J.,XIE W.,WU Q.-J.,WANG S.-L.. 2023

[6]Antennal transcriptome analysis of odorant-binding proteins and characterization of GOBP2 in the variegated cutworm Peridroma saucia. Jun Feng Dong,Ke Wang,Ya Lan Sun,Cai Hong Tian,Shao Li Wang. 2023

[7]Potential role of the TuCCE50 gene in abamectin resistance in field populations of Tetranychus urticae Koch. Tian, Tian,Wang, Ke,Zhang, Yan,Guo, Zhaojiang,Zhang, Youjun,Wang, Shaoli. 2025

[8]Prokaryotic Expression and Binding Characteristics of Odor-Binding Protein GqinOBP10 in Gynaephora qinghaiensis. Zhanling Liu,Dejing Tang,Youpeng Lai,Shujing Gao,Haibin Han,Yuantao Zhou. 2025

[9]六斑平颜蚜蝇雄虫对雌性腺化合物的触角电位和行为反应. 李彦如,史丽,韩海斌,刘爱萍,陈国泽. 2023

[10]Transcriptome Analysis Of Ophraella Communa Male Reproductive Tract In Indirect Response To Elevated Co2 And Heat Wave. Gao, XY, Tian, ZY, Zhang, Y, Chen, GM, Ma, C, Tian, ZQ, Cui, SW, Lu, YY, Zhou, ZS. 2020

[11]Behavioral and electrophysiological responses of natural enemies to synomones from tea shoots and kairomones from tea aphids, Toxoptera aurantii. Han, BY,Chen, ZM. 2002

[12]Assessing Artemisia lavandulaefolia as a trap plant for managing Apolygus lucorum in tea plantations. Tian, Yueyue,Ping, Sun,Zhang, Xinzhe,Li, Bingqian,Zhang, Zhengqun,Cai, Xiaoming. 2023

[13]Mixture of Synthetic Plant Volatiles Attracts More Stick Tea Thrips Dendrothrips minowai Priesner (Thysanoptera: Thripidae) and the Application as an Attractant in Tea Plantations. Zhengwei Xu,Guowei Zhang,Yan Qiu,Zongxiu Luo,Xiaoming Cai,Zhaoqun Li,Lei Bian,Nanxia Fu,Li Zhou,Fida Hussain Magsi,Zongmao Chen,Xiaoming Zhang,Chunli Xiu. 2024

[14]Electrophysiological and Behavioral Responses of Thrips hawaiiensis (Thysanoptera: Thripidae) to the Floral Volatiles of the Horticultural Plant Magnolia grandiflora (Magnoliales: Magnoliaceae). Tao Zhang,Yuping Yang,Filippo Maggi,Feiyu Jiang,Rongrong Yuan,Lujie Huang,Xueyan Zhang,Yu Cao,Yulin Gao. 2025

[15]Optimization and field evaluation of sex-pheromone of potato tuber moth, Phthorimaea operculella Zeller (Lepidoptera: Gelechiidae). Yan, Junjie,Zhang, Mengdi,Ali, Abid,Du, Xia,Mei, Xiangdong,Gao, Yulin. 2022

[16]Identification of Key Headspace Volatile Compounds Signaling Preference for Rice over Corn in Adult Females of the Rice Leaf Folder Cnaphalocrocis medinalis. Chen, Ping,Dai, Changgen,Liu, Huan,Hou, Maolin. 2022

[17]Mutagenesis of odorant coreceptor Orco reveals the distinct role of olfaction between sexes in Spodoptera frugiperda. Song CAO,Dong dong SUN,Yang LIU,Qing YANG,Gui rong WANG. 2023

[18]Expression profiles and functional prediction of ionotropic receptors in Asian corn borer, Ostrinia furnacalis (Lepidoptera: Crambidae). 张玉,,杨斌,,于洁,,庞保平,,王桂荣. 2022

[19]Synthesis and bioactivity studies of the potato tuber moth, Phthorimaea operculella Zeller (Lepidoptera: Gelechiidae) sex pheromone analogs. Yan, Junjie,Zhang, Mengdi,Li, Jing,Rondon, Silvia I.,Gao, Yulin. 2024

[20]The influence of IR25a on electrophysiology and reproductive behaviour in the oriental fruit fly, Bactrocera dorsalis. Qian, Qian,Yuan, Jinxi,Zhang, Jie,Wang, Qi,Yan, Shanchun,Liu, Wei,Wang, Guirong. 2025

作者其他论文 更多>>