数字农科院2.0

Chromosome-level genome assembly of the parasitoid wasp Aenasius arizonensis

文献类型： 外文期刊

作者： Wan Ying Dong;Tian Yu Huang;Sheng Yuan Zhao;Juan Zhang;Yang Lei;Jun Huang;Zhong Shi Zhou;Yao Bin Lu

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期刊名称： Scientific Data

ISSN： 2052-4463

年卷期： 2025 年 12 卷 1 期

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收录情况： SCIE（2025版）

摘要： Aenasius arizonensis is an important solitary endoparasitoid successfully used for biocontrol of cotton mealybug. However, lacking genomic resources has limited molecular-level investigations. Our exploration produced a superior genomic assembly of A. arizonensis from the chromosome level by combining MGISEQ short reads, Hi-C scaffolding, and PacBio Revio sequencing techniques. The genome measured 398.69 Mb, including a contig N50 of 4.73 Mb, a BUSCO completeness level of 97.07%, and a scaffold N50 of 35.96 Mb. Hi-C data were further utilized cluster and anchor 98.66% of the genome sequences into 11 chromosomes. Approximately, 165.90 Mb, representing about 41.61% of the genome, was identified as repeat elements. Non-coding sequence annotation identified 171 rRNAs, 117 small RNAs, 331 regulatory RNAs, and 872 tRNAs. Genome annotation reveals 11,727 protein-coding genes, with 10,842 (92.45%) genes functionally annotated. In summary, our chromosome-level genome assembly serves as a significant resource for advancing research on Encyrtidae parasitoids.

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