数字农科院2.0

Real-Time PCR Detection of Adulteration of Olive Oil With Soybean Oil Based on Optimized CTAB DNA Extraction Method

文献类型： 外文期刊

作者： Kai Chen;Jian Zhi Luo;Bin Xiao;Xue Wei Yuan;Juan Zhang;Nan Wang;Li Qiang Liu;Ai Ke Li;Ailiang Chen

作者机构：

关键词： adulteration;authenticity;DNA extraction;edible oils

期刊名称： Journal of Food Process Engineering

ISSN： 0145-8876

年卷期： 2026 年 49 卷 1 期

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： This study established an optimized CTAB-based DNA extraction method for edible oils, which enables stable and reliable DNA recovery from commercially available edible oils for authenticity identification and adulteration detection. Real-time PCR was used to assess DNA precipitation time and organic solvent extraction, revealing that longer precipitation increased inhibitors, hindering PCR amplification, while organic solvents enhanced efficiency. The method was applied to detect adulteration in commercially available soybean and olive oils. A standard curve based on real-time PCR Ct values demonstrated the ability to detect as low as 10% soybean oil in olive oil, with recovery rates of 92% and 80% for samples spiked with 70% and 30% soybean oil, respectively. The coefficients of variation (CV) were 11% and 14%, within acceptable ranges. This reliable method provides a valuable tool for edible oil authentication and adulteration detection.

分类号：

  • 相关文献

[1]Impact of amplicon length on the identification of edible oils based on real-time PCR. Kai Chen,Bin Xiao,Jian Zhi Luo,Xue Wei Yuan,Juan Zhang,Songxue Wang,Rui Zhang,Nan Wang,Li Qiang Liu,Ai Ke Li,Ailiang Chen. 2025

[2]Droplet digital polymerase chain reaction (ddPCR) for rapid screening of adulterants in honey: A case study on acacia honey adulterated with canola honey. Zhaotong You,Yingting Mei,Xiaofu Wang,Xiaoyun Chen,Junfeng Xu. 2021

[3]Quantitation of triacylglycerols in edible oils by off-line comprehensive two-dimensional liquid chromatography-atmospheric pressure chemical ionization mass spectrometry using a single column. Wei, Fang,Hu, Na,Lv, Xin,Dong, Xu-Yan,Chen, Hong,Wei, Fang,Hu, Na,Lv, Xin,Dong, Xu-Yan,Chen, Hong.

[4]Accurate quantification of TAGs to identify adulteration of edible oils by ultra-high performance liquid chromatography-quadrupole-time of flight-tandem mass spectrometry. Wei H.,Yang D.,Mao J.,Zhang Q.,Cheng L.,Yang X.,Li P.. 2023

[5]Recent advancements in detecting sugar-based adulterants in honey - A challenge. Wu, Liming,Du, Bing,Chen, Lanzhen,Zhao, Liuwei,Wang, Miao,Xue, Xiaofeng,Wu, Liming,Chen, Lanzhen,Wang, Miao,Xue, Xiaofeng,Wu, Liming,Chen, Lanzhen,Wang, Miao,Xue, Xiaofeng,Vander Heyden, Yvan.

[6]Quantitative determination of soybean meal content in compound feeds: comparison of near-infrared spectroscopy and real-time PCR. Li, Hui,Lv, Xiaowen,Wang, Jing,Li, Junguo,Yang, Haifeng,Qin, Yuchang.

[7]Wheat authentication:An overview on different techniques and chemometric methods. Liu, Hong-Yan,Wadood, Syed Abdul,Xia, Yu,Liu, Yi,Guo, Huan,Guo, Bo-Li,Gan, Ren-You. 2021

[8]Do-it-yourself Molecular Biology for Plant Breeding: Low-cost Tools for Developing Countries. Huynh, O. A.,Hofinger, B. J.,Jankowicz-Cieslak, J.,Forster, B. P.,Till, B. J.,Beshir, M. M.,Guo, H.. 2013

[9]Isolation and PCR detection of foreign DNA sequences in bee honey raised on genetically modified Bt (Cry1Ac) cotton. Jin, W.,Wu, H.,Wang, F.,You, C.,Peng, Y.,Jia, S.. 2007

[10]Comparison of Three DNA Extraction Methods for Feed Products and Four Amplification Methods for the 5 '-Junction Fragment of Roundup Ready Soybean. Wang, Xiumin,Teng, Da,Tian, Fang,Guan, Qingfeng,Wang, Jianhua,Wang, Xiumin,Teng, Da,Tian, Fang,Guan, Qingfeng,Wang, Jianhua. 2012

[11]Effects of energy-gathered and energy-divergent ultrasound on structure, DNA extraction and adulterated quantification of sweet potato vermicelli and its starch. Muyembe, Diana Kavidia,Zhang, Miao,Sun, Hong-Nan,Mu, Tai-Hua. 2024

[12]Rapid Magnetic Solid-Phase Extraction Based on Monodisperse Magnetic Single-Crystal Ferrite Nanoparticles for the Determination of Free Fatty Acid Content in Edible Oils. Wei, Fang,Lv, Xin,Dong, Xu-Yan,Chen, Hong,Zhao, Qin,Feng, Yu-Qi.

[13]The impact of technical cashew nut shell liquid on thermally-induced trans isomers in edible oils. Guo, Qin,Wang, Feng,He, Fan,Ha, Yi-ming,Li, Qing-peng,Jin, Jing,Deng, Zhao-xuan.

[14]Dispersive microextraction based on water-coated Fe3O4 followed by gas chromatography-mass spectrometry for determination of 3-monochloropropane-1,2-diol in edible oils. Zhao, Qin,Xiao, Neng,Yu, Qiong-Wei,Yuan, Bi-Feng,Feng, Yu-Qi,Wei, Fang. 2012

[15]Rapid determination of trans-resveratrol in vegetable oils using magnetic hydrophilic multi-walled carbon nanotubes as adsorbents followed by liquid chromatography-tandem mass spectrometry. Ma, Fei,Li, Peiwu,Zhang, Qi,Yu, Li,Zhang, Liangxiao,Ma, Fei,Li, Peiwu,Zhang, Qi,Yu, Li,Zhang, Liangxiao,Ma, Fei,Li, Peiwu,Zhang, Qi,Yu, Li,Zhang, Liangxiao,Ma, Fei,Li, Peiwu,Yu, Li,Zhang, Liangxiao,Li, Peiwu,Zhang, Qi.

[16]The application of emerging technologies for the quality and safety evaluation of oilseeds and edible oils. Xue Li,Wenwen Liu,Lu Xiao,Jie Zhao,Yan Chen,Liangxiao Zhang,Peiwu Li,Dolores Pérez-Marín,Xu Wang. 2025

[17]A polarity-sensitive fluorescent probe for monitoring liver disease and edible oil quality. Kaifu Ma,Peng Zhang,Yu Zhang,Mingyue Zheng,Na Xiao,Zhiman Li,Wei Shu. 2025

[18]Analysis of maltooligosaccharides in honey samples by ultra-performance liquid chromatography coupled with evaporative light scattering detection. Zhou, Jinhui,Wu, Liming,Diao, Qingyun,Li, Yi,Zhao, Jing,Zhou, Jinhui,Wu, Liming,Li, Yi,Zhao, Jing,Zhou, Jinhui,Wu, Liming,Li, Yi,Zhao, Jing,Qi, Yitao,Ritho, Joan,Duan, Lili.

[19]Research Progress on Traceability and Authenticity of Beef. Bai, Yang,Liu, Haijin,Zhang, Bin,Zhang, Jiukai,Wu, Hao,Zhao, Shanshan,Qie, Mengjie,Guo, Jun,Wang, Qian,Zhao, Yan. 2021

[20]A review of fruit juice authenticity assessments: Targeted and untargeted analyses. Lei Xu,Zhenzhen Xu,Xiaojun Liao. 2021

作者其他论文 更多>>