数字农科院2.0

Molecular Characteristics and Promoter Analysis of Porcine COL1A1

文献类型： 外文期刊

作者： Xiang G.;Huang L.;Zhang X.;Wang N.;Wang H.;Mu Y.;Li K.;Liu Z.

作者机构：

关键词： （2-0-2）COL1A1;core promoter;luciferase assay;pig;protein structure and function

期刊名称： Genes

ISSN： 2073-4425

年卷期： 2024 年 13 卷 11 期

页码：

收录情况： SCIE（2024版）

摘要： COL1A1 encodes the type I collagen α1 chain, which shows the highest abundance among members of the collagen family and is widely expressed in different mammalian cells and tissues. However, its molecular characteristics are not completely elucidated. In this study, the molecular profiles of COL1A1 and characteristics of the COL1A1 protein were investigated using a promoter activity assay and multiple bioinformatics tools. The results showed that the 5′ flanking region of porcine COL1A1 contained two CpG islands, five core promoter sequences, and twenty-six transcription factor-binding sites. In the luciferase assay, the upstream 294 bp region of the initiation codon of COL1A1 showed the highest activity, confirming that this section is the core region of the porcine COL1A1 promoter. Bioinformatic analysis revealed that COL1A1 is a negatively charged, hydrophilic secreted protein. It does not contain a transmembrane domain and is highly conserved in humans, mice, sheep, and pigs. Protein interaction analysis demonstrated that the interaction coefficient of COL1A1 with COL1A2, COL3A1, ITGB1, and ITGA2 was greater than 0.9, suggesting that this protein plays a crucial role in collagen structure formation and cell adhesion. These results provide a theoretical basis for further investigation of the functions of porcine COL1A1. © 2022 by the authors.

分类号：

  • 相关文献

[1]Molecular Characteristics and Promoter Analysis of Porcine COL1A1. Xiang G.,Huang L.,Zhang X.,Wang N.,Wang H.,Mu Y.,Li K.,Liu Z.. 2022

[2]Determination of genetic effects and functional SNPs of bovine HTR1B gene on milk fatty acid traits. Mingyue Cao,Lijun Shi,Peng Peng,Bo Han,Lin Liu,Xiaoqing Lv,Zhu Ma,Shengli Zhang,Dongxiao Sun. 2021

[3]Individual-breed assignment analysis in swine populations by using microsatellite markers. Fan, B,Chen, YZ,Moran, C,Zhao, SH,Liu, B,Yu, M,Zhu, MJ,Xiong, TA,Lil, K. 2005

[4]Genome-Wide Association Study for Certain Carcass Traits and Organ Weights in a Large WhitexMinzhu Intercross Porcine Population. Liu Xin,Wang Li-gang,Liang Jing,Yan Hua,Zhao Ke-bin,Li Na,Zhang Long-chao,Wang Li-xian,Li Na. 2014

[5]Molecular characterization and association analysis of FBXO40 with partial hematological indexes in pig. Wang, Z. W.,Li, X. Y.,Tang, Z. L.,Yang, S. L.,Ying, Z. Z.,Fu, T.,Mu, Y. L.,Ao, H.,Li, K.,Wang, Z. W.,Li, X. Y.,Ying, Z. Z.,Fu, T.,Fan, B.. 2010

[6]The microRNA expression profile in porcine skeletal muscle is changed by constant heat stress. Hao, Y.,Liu, J. R.,Yang, P. G.,Feng, Y. J.,Cui, Y. J.,Yang, C. H.,Gu, X. H.,Liu, J. R.,Zhang, Y..

[7]Porcine LEM domain-containing 3: Molecular cloning, functional characterization, and polymorphism associated with ear size. Liang Jing,Li Na,Zhang Long-chao,Wang Li-gang,Liu Xin,Zhao Ka-bin,Yan Hua,Pu Lei,Zhang Yue-bo,Shi Hui-bi,Wang Li-xian,Li Na,Zhang Qin. 2016

[8]Genetic Variation of EPAS1 Gene in Tibetan Pigs and Three Low-Altitude Pig Breeds in China. Dong Kun-zhe,Yao Na,Shu Guo-tao,Zhao Qian-jun,Ma Yue-hui,Kang Ye,Zuo Qing-qing. 2014

[9]Effect of Graded Levels of Fiber from Alfalfa Meal on Nutrient Digestibility and Flow of Fattening Pigs. Chen Liang,Gao Li-xiang,Zhang Hong-fu. 2014

[10]Seroprevalence and genotypes of Toxoplasma gondii isolated from pigs intended for human consumption in Liaoning province, northeastern China. Wang, Dawei,Zhang, Guoxin,Yuan, Gaoming,He, Jianbin,Yang, Na,Liu, Yan,Jiang, Tiantian,Su, Chunlei. 2016

[11]Chromosome Mapping, Expression and Polymorphism Analysis of CRABP1 Gene in Pigs. Zhao Shuan-ping,Tang Zhong-lin,Zhou Rong,Li Kui,Zheng Jian-wei,Qu Chang-qing,Zhao Shuan-ping. 2014

[12]Effect of graded levels of fiber from alfalfa meal on apparent and standardized ileal digestibility of amino acids of growing pigs. Chen Liang,Gao Li-xiang,Liu Li,Ding Ze-ming,Zhang Hong-fu,Chen Liang. 2015

[13]Effect of graded levels of fiber from alfalfa meal on intestinal nutrient and energy flow, and hindgut fermentation in growing pigs. Chen, L.,Zhang, H. F.,Gao, L. X.,Zhao, F.,Lu, Q. P.,Sa, R. N.. 2013

[14]Effects of Long-Term Protein Restriction on Meat Quality, Muscle Amino Acids, and Amino Acid Transporters in Pigs. Yin, Jie,Li, Yuying,Zhu, Xiaotong,Han, Hui,Ren, Wenkai,Chen, Shuai,Bin, Peng,Liu, Gang,Li, Tiejun,Yin, Yulong,Yin, Jie,Li, Yuying,Zhu, Xiaotong,Han, Hui,Ren, Wenkai,Chen, Shuai,Bin, Peng,Liu, Gang,Li, Tiejun,Yin, Yulong,Yin, Jie,Li, Yuying,Zhu, Xiaotong,Han, Hui,Ren, Wenkai,Chen, Shuai,Bin, Peng,Liu, Gang,Li, Tiejun,Yin, Yulong,Yin, Jie,Li, Yuying,Zhu, Xiaotong,Han, Hui,Ren, Wenkai,Chen, Shuai,Bin, Peng,Liu, Gang,Li, Tiejun,Yin, Yulong,Yin, Jie,Li, Yuying,Han, Hui,Ren, Wenkai,Chen, Shuai,Bin, Peng,Huang, Xingguo,Fang, Rejun,Huang, Xingguo,Fang, Rejun,Li, Tiejun,Yin, Yulong,Wang, Bin,Wang, Kai,Sun, Liping.

[15]Timing of The First Zygotic Cleavage as a Developmental Potential Marker for Porcine Cloned Embryos. Luo Xue-Ming,Feng Chong,Long Chuan,Yan Jun,Pan Deng-Ke,Xiao Wei,Xue Zhen-Hua,Yun Peng. 2010

[16]Genome-wide profiling of Sus scrofa circular RNAs across nine organs and three developmental stages. Liang, Guoming,Yang, Yalan,Niu, Guanglin,Tang, Zhonglin,Li, Kui,Liang, Guoming,Yang, Yalan,Tang, Zhonglin,Li, Kui,Liang, Guoming,Yang, Yalan,Tang, Zhonglin.

[17]Investigation on the co-infections of Toxoplasma gondii with PRRSV, CSFV or PCV-2 in swine in part of China. Wang Shuai,Zhang Meng,Liu Xin-chao,Lin Tao,Zhao Guang-wei,Ia Hassan,Yan Ruo-feng,Song Xiao-kai,Li Xiang-rui,Yang Han-chun,Lin Tao,Yuan Shi-shan. 2015

[18]Genetic characterization of Toxoplasma gondii from pigs from different localities in China by PCR-RFLP. Jiang, Hai-Hai,Huang, Si-Yang,Zhou, Dong-Hui,Zhang, Xiao-Xuan,Su, Chunlei,Zhu, Xing-Quan,Deng, Shun-Zhou,Su, Chunlei,Zhang, Xiao-Xuan. 2013

[19]Genome-wide association study for rib eye muscle area in a Large WhitexMinzhu F-2 pig resource population. Guo Yun-yan,Liu Wen-zhong,Guo Yun-yan,Zhang Long-chao,Wang Li-xian. 2015

[20]Energy, amino acid, and phosphorus digestibility of phytase transgenic corn for growing pigs. Li, S. F.,Niu, Y. B.,Feng, M. S.,Du, B.,Liu, J. S.,Lu, L.,Zhang, L. Y.,Ran, C. Y.,Luo, X. G.,Liu, J. S.,Deng, J. L.. 2013

作者其他论文 更多>>