STnc1280, a trans-coding sRNA is involved in virulence modulation via targeting gldA mRNA in Salmonella Typhimurium
文献类型： 外文期刊
作者： Ning C.;Li N.;Wang L.;Guo Y.;Ji C.;Li Z.;Shang Y.;Zhang X.;Sun Y.;Huang X.;Leng Q.;Cai X.;Meng Q.;Qiao J.
作者机构：
关键词： modulation;Salmonella Typhimurium;sRNA;STnc1280;virulence
期刊名称： Journal of Medical Microbiology
ISSN： 0022-2615
年卷期： 2024 年 73 卷 2 期
页码：
收录情况： SCIE（2024版）
摘要： Introduction. Salmonella Typhimurium (STM) is a food-borne Gram-negative bacterium, which can infect humans and a wide range of livestock and poultry, causing a variety of diseases such as septicaemia, enteritis and abortion. Hypothesis/Gap Statement. We will decipher the impacts of sRNA STnc1280 on STM virulence and provide a theoretical basis to reveal the regulatory role and molecular mechanism of STnc1280. Aim. The main objective of this study was to clarify whether sRNA STnc1280 exerts regulatory roles on STM pathogenicity. Methodology. The STnc1280 gene was amplified and its molecular characteristics were analysed in this study. Then, STnc1280 gene deletion strain (STM-ΔSTnc1280) and the complementary strain (ΔSTnc1280/STnc1280) were constructed by λ-Red homologous recombination method, respectively, to analyse of adhesion and invasive ability and pathogenicity of different strains. Subsequently, the potential target gene regulated by STnc1280 was predicted using target RNA2 software, followed by the verification of the interaction between STnc1280 and target mRNA using the dual plasmid reporter system (DPRS). Furthermore, the mRNA and protein level of target gene was determined using qRT-PCR and Western blot, respectively. Results. The results revealed that the cell adhesion and invasive ability and pathogenicity of STM-ΔSTnc1280 were significantly reduced compared to STM-SL1344 strain, indicating that the deficiency of STnc1280 gene significantly influenced STM pathogenicity. The DPRS results showed that STnc1280 can interact with the mRNA of target gene gldA, thus suppressing the expression of lacZ gene. Furthermore, the level of gldA mRNA was not influenced in STM-ΔSTnc1280, but the expression of GldA protein decreased significantly. Conclusion. Combining the bioinformatic analysis, these findings suggested that STnc1280 may bind to the SD sequence of gldA mRNA, hindering the binding of ribosomes to gldA mRNA, thereby inhibiting the expression of GldA protein to modulate the virulence of STM. © 2024 The Authors.
分类号：
- 相关文献
作者其他论文 更多>>
-
Spatiotemporal patterns and driving factors of gross primary productivity over the Mongolian Plateau steppe in the past 20 years
作者：Ding L.;Li Z.;Wang X.;Shen B.;Xiao L.;Dong G.;Yu L.;Nandintsetseg B.;Shi Z.;Chang J.;Shao C.
关键词：Climatic factors;Grassland;Gross primary productivity;Human activity;Mongolian Plateau
-
Both yields of maize and soybean and soil carbon sequestration in typical Mollisols cropland decrease under future climate change: SPACSYS simulation
作者：Liang S.;Sun N.;Longdoz B.;Meersmans J.;Ma X.;Gao H.;Zhang X.;Qiao L.;Colinet G.;Xu M.;Wu L.
关键词：carbon sequestration efficiency;climate change;long-term fertilization;Mollisols;SOC stock;SPACSYS model;yield
-
Transcriptome-Wide Identification of Cytochrome P450s in Tea Black Tussock Moth (Dasychira baibarana) and Candidate Genes Involved in Type-II Sex Pheromone Biosynthesis
作者：Wang T.;Liu X.;Luo Z.;Cai X.;Li Z.;Bian L.;Xiu C.;Chen Z.;Li Q.;Fu N.
关键词：biosynthesis;cytochrome P450s;phylogenetic analysis;RNAseq;tea black tussock moth;Type-II sex pheromone
-
Pan-genome and phylogenomic analyses highlight Hevea species delineation and rubber trait evolution
作者：Fang Y.;Xiao X.;Lin J.;Lin Q.;Wang J.;Liu K.;Li Z.;Xing J.;Liu Z.;Wang B.;Qi Y.;Long X.;Zeng X.;Hu Y.;Qi J.;Qin Y.;Yang J.;Zhang Y.;Zhang S.;Ye D.;Zhang J.;Liu J.;Tang C.
关键词：
-
Evaluation of Loquat Jam Quality at Different Cooking Times Based on Physicochemical Parameters, GC-IMS and Intelligent Senses
作者：Qiao M.;Xiong H.;Cai X.;Jiang Y.;Zhao X.;Miao B.
关键词：cooking time;GC-IMS;intelligent senses;loquat jam;physicochemical parameters
-
MscI restriction enzyme cooperating recombinase-aided isothermal amplification for the ultrasensitive and rapid detection of low-abundance EGFR mutations on microfluidic chip
作者：Xu S.;Wang X.;Wu C.;Zhu X.;Deng X.;Wu Y.;Liu M.;Huang X.;Wu L.;Huang H.
关键词：（2-0-2）CRISPR-Cas12a;DNA point mutation;Lung cancer;Microfluidic chip;MscI restriction enzyme
-
Modulation of pectin on intestinal barrier function via changes in microbial functional potential and bile acid metabolism
作者：Yin C.;Wen X.;Dang G.;Zhong R.;Meng Q.;Feng X.;Liu L.;Wu S.;He J.;Chen L.;Zhang H.
关键词：bile acid;ileal microbiota;intestinal health;pectin