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Purification And Characterization Of The Major Protein Isolated From Semen Armeniacae Amarum And The Properties Of Its Thermally Induced Nanoparticles

文献类型： 外文期刊

作者： Lin, D; Lin, W; Gao, GZ; Zhou, JW; Chen, TB; Ke, LJ; Rao, PF; Wang, Q

作者机构：

关键词： Protein Nanoparticles; Thermal Assembly; Disulfide Bond

期刊名称： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

ISSN： 0141-8130

年卷期： 2020 年 159 卷

页码：

收录情况： JCR（2021版）

摘要： From the aqueous extract of Semen Armeniacae Amarum, a major protein isolate was purified and characterized as a novel member of the 11S globulin family, which is composed of three polypeptides linked by disulfide bond. Furthermore, the feasibility of using the isolated protein for fabricating nanocarriers was investigated. The results indicate that thermal treatment of the globulin induced the rearrangement of the disulfide bond to form homodimers of acid polypeptides during the formation of nanoparticles. The harvested nanoparticles produced by heat-induced assembly are spherical in shape, with an average size of 92 nm and exhibited low cytotoxicity to L-02 and MDCK cell lines. These nanoparticles are capable to encapsulate paclitaxel, estimated the maximum encapsulation efficiency of paclitaxel loaded to the nanoparticles was 92.6% and the maximum release of paclitaxel was 57.4%. This research suggests that the screening of traditional herbal extracts could provide a novel source of protein nanocarriers. (C) 2020 Elsevier B.V. All rights reserved.

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