Functional Characterization Of One Sex Pheromone Receptor (Alucor4) In Apolygus Lucorum (Meyer-Dur)
文献类型: 外文期刊
作者: An, XK; Khashaveh, A; Liu, DF; Xiao, Y; Wang, Q; Wang, SN; Geng, T; Gu, SH; Zhang, YJ
作者机构:
关键词: Apolygus Lucorum; Sex Pheromone Receptor; Alucor4; Xenopus Oocyte Recording; Rnai; Electroantennogram (Eag)
期刊名称: JOURNAL OF INSECT PHYSIOLOGY
ISSN: 0022-1910
年卷期: 2020 年 120 卷
页码:
收录情况: JCR(2021版)
摘要: Traps baited with female-produced sex pheromones have been very effective in the monitoring and management of mirid bugs in numerous field trials. However, none of the target odorant receptors for sex pheromone components in Apolygus lucorum have been identified. Here, we identified one candidate sex pheromone receptor, AlucOR4, from A. lucorum. Quantitative real-time PCR (qPCR) analysis revealed that AlucOR4 was antennae-enriched and male-biased in adult A. lucorum. Xenopus oocyte expression system assays demonstrated that AIucOR4/AlucOrco was sensitive to two major sex pheromone constituents and exhibited high sensitivity to (E)2-hexenyl butyrate (E2HB) and lower sensitivity to hexyl butyrate (HB). The expression level of target mRNA was significantly reduced ( > 80%) in dsAlucOR4-injected bugs after five days. The electroantennogram (EAG) responses of male antennae to E2HB and HB were also reduced significantly (similar to 40%). Our findings suggest that AlucOR4 is essential to sex pheromone perception in A. lucorum.
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