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Molecular Characterization Of An Nadph Cytochrome P450 Reductase From Bemisia Tabaci Q: Potential Involvement In Susceptibility To Imidacloprid

文献类型： 外文期刊

作者： He, C; Liang, JJ; Liu, SN; Zeng, Y; Wang, SL; Wu, QJ; Xie, W; Zhang, YJ

作者机构：

关键词： Bemisia Tabaci; Cpr; Imidacloprid; Rnai

期刊名称： PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY

ISSN： 0048-3575

年卷期： 2020 年 162 卷

页码：

收录情况： JCR（2021版）

摘要： NADPH cytochrome P450 reductase (CPR) is an integral component of cytochrome P450-mediated biological reactions, such as the metabolism of xenobiotics, including insecticides. CPR has been reported to be associated with insecticide tolerance in several insects. However, the biochemical characteristics and biological function of CPR in Bemisia tabaci Q (BtCPR) remain undefined. In this study, BtCPR was cloned, and bioinformatic analysis showed that BtCPR is a transmembrane protein with a molecular weight (MW) of 76.73 kDa and contains conserved binding domains (FMN, FAD, and NADPH). Tissue- and developmental stage-dependent expression indicated that the highest expression levels of BtCPR occurred in head tissue and in male adults. Transcripts of BtCPR in the field B. tabaci Q strain were 1.62-fold higher than those of the laboratory B. tabaci Q strain. In both field and laboratory adults, the susceptibility of BtCPR-knockdown B. tabaci Q to imidacloprid substantially increased compared to that of the B. tabaci Q control group. Furthermore, the heterologous expression of BtCPR in Sf9 cells exhibited catalytic activity for cytochrome c reduction, following Michaelis-Menten kinetics. Sf9 cells overexpressing BtCPR had greater viability than the control cells when treated with imidacloprid. The results suggest that BtCPR could affect the susceptibility of B. tabaci Q to imidacloprid and could also be considered a novel target for pest control.

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