数字农科院2.0

Fine mapping of the major QTLs for biochemical variation of sulforaphane in broccoli florets using a DH population

文献类型： 外文期刊

作者： Li Zhansheng;Liu Yumei;Yuan Suxia;Han Fengqing;Fang Zhiyuan;Yang Limei;Zhuang Mu;Zhang Yangyong;Lv Honghao;Wang Yong;Ji Jialei

作者机构：

期刊名称： Scientific reports

ISSN： 2045-2322

年卷期： 2021 年 11 卷 1 期

页码：

收录情况： JCR（2021版）

摘要： Glucoraphanin is a major secondary metabolite found in Brassicaceae vegetables, especially broccoli, and its degradation product sulforaphane plays an essential role in anticancer. The fine mapping of sulforaphane metabolism quantitative trait loci (QTLs) in broccoli florets is necessary for future marker-assisted selection strategies. In this study, we utilized a doubled haploid population consisting of 176 lines derived from two inbred lines (86,101 and 90,196) with significant differences in sulforaphane content, coupled with extensive genotypic and phenotypic data from two independent environments. A linkage map consisting of 438 simple sequence repeats markers was constructed, covering a length of 1168.26 cM. A total of 18 QTLs for sulforaphane metabolism in broccoli florets were detected, 10 were detected in 2017, and the other 8 were detected in 2018. The LOD values of all QTLs ranged from 3.06 to 14.47, explaining 1.74-7.03% of the biochemical variation between two years. Finally, 6 QTLs (qSF-C3-1, qSF-C3-2, qSF-C3-3, qSF-C3-5, qSF-C3-6 and qSF-C7) were stably detected in more than one environment, each accounting for 4.54-7.03% of the phenotypic variation explained (PVE) and a total of 30.88-34.86% of PVE. Our study provides new insights into sulforaphane metabolism in broccoli florets and marker-assisted selection breeding in Brassica oleracea crops.

分类号：

  • 相关文献
作者其他论文 更多>>