数字农科院2.0

Two user-friendly molecular markers developed for the identification of hybrid lethality genes in brassica oleracea

文献类型： 外文期刊

作者： Xiao Zhiliang;Kong Congcong;Han Fengqing;Yang Limei;Zhuang Mu;Zhang Yangyong;Wang Yong;Ji Jialei;Li Zhansheng;Fang Zhiyuan;Lv Honghao

作者机构：

关键词： Brassica oleracea; Breeding; Hybrid lethality; InDel marker; KASP marker

期刊名称： Agronomy

ISSN： 2073-4395

年卷期： 2021 年 11 卷 5 期

页码：

收录情况： JCR（2021版）

摘要： Cabbage (Brassica oleracea) is an important vegetable crop that is cultivated worldwide. Previously, we reported the identification of two dominant complementary hybrid lethality (HL) genes in cabbage that could result in the death of hybrids. To avoid such losses in the breeding process, we attempted to develop molecular markers to identify HL lines. Among 54 previous mapping markers closely linked to BoHL1 or BoHL2, only six markers for BoHL2 were available in eight cabbage lines (two BoHL1 lines; three BoHL2 lines; three lines without BoHL); however, they were neither universal nor user-friendly in more inbred lines. To develop more accurate markers, these cabbage lines were resequenced at an ~20× depth to obtain more nucleotide variations in the mapping regions. Then, an InDel in BoHL1 and a single-nucleotide polymorphism (SNP) in BoHL2 were identified, and the corresponding InDel marker MBoHL1 and the competitive allele-specific PCR (KASP) marker KBoHL2 were developed and showed 100% accuracy in eight inbred lines. Moreover, we identified 138 cabbage lines using the two markers, among which one inbred line carried BoHL1 and 11 inbred lines carried BoHL2. All of the lethal line genotypes obtained with the two markers matched the phenotype. Two markers were highly reliable for the rapid identification of HL genes in cabbage. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

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