数字农科院2.0

Chromosome-level genome assembly of the spotted alfalfa aphid Therioaphis trifolii

文献类型： 外文期刊

作者： Tianyu Huang;Yang Liu;Kang He;Frédéric Francis;Bing Wang;Guirong Wang

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期刊名称： Scientific Data

ISSN： 2052-4463

年卷期： 2023 年 10 卷 1 期

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收录情况： SCIE（2023版）

摘要： The spotted alfalfa aphid (SAA, Therioaphis trifolii) (Hemiptera: Aphididae) is a destructive pest of cultivated alfalfa (Medicago sativa L.) that leads to large financial losses in the livestock industry around the world. Here, we present a chromosome-scale genome assembly of T. trifolii, the first genome assembly for the aphid subfamily Calaphidinae. Using PacBio long-read sequencing, Illumina sequencing, and Hi-C scaffolding techniques, a 541.26 Mb genome was generated, with 90.01% of the assembly anchored into eight scaffolds, and the contig and scaffold N50 are 2.54 Mb and 44.77 Mb, respectively. BUSCO assessment showed a completeness score of 96.6%. A total of 13,684 protein-coding genes were predicted. The high-quality genome assembly of T. trifolii not only provides a genomic resource for the more complete analysis of aphid evolution, but also provides insights into the ecological adaptation and insecticide resistance of T. trifolii.

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