数字农科院2.0

Functional characterization of fatty acid desaturases and dietary lipid sources modulation of fatty acid conversion in largemouth bass (Micropterus salmoides)

文献类型： 外文期刊

作者： Yangyang Liu;Rudy Caparros Megido;Frédéric Francis;Jie Wang;Hao Wang;Liang Hu;Xiaofang Liang;Min Xue

作者机构：

关键词： DHA inclusion;fatty acid desaturases;Micropterus salmoides;n-3 LC-PUFA biosynthesis;oil sources

期刊名称： Aquaculture

ISSN： 0044-8486

年卷期： 2026 年 615 卷

页码：

收录情况： SCIE（2025版）

摘要： To evaluate n-3 long-chain polyunsaturated fatty acids (LC-PUFA) biosynthesis capacity and the selected dietary lipid sources effects in largemouth bass (Micropterus salmoides), we combined functional characterization of fatty acid desaturase genes with an 8-week feeding trial. Yeast expression identified three candidate desaturase genes: fads2 (fatty acid desaturase 2) catalyzing C18:3n-3 → C18:4n-3, and delta4 fads (acyl-CoA Delta4 desaturase-like) exhibiting dual Δ5/Δ4 activity (C20:4n-3 → C20:5n-3; C22:5n-3 → C22:6n-3), and fads6 (fatty acid desaturase 6) showed no detectable desaturase activity. In a 3 × 2 factorial design, 450 juvenile largemouth bass (17.99 ± 0.02g, 25 fish per tank, triplicate) received diets containing—linseed oil (LO, 54.24% ALA), cottonseed oil (CO, 0.14% ALA) or black soldier fly oil (BSFO, 2.28% ALA), with or without DHA inclusion (3% Schizochytrium sp. meal). Two-way ANOVA revealed that LO and BSFO improved growth (FBW, WG, SGR) over CO, and LO raised tissue n-3 PUFAs levels aboved CO and BSFO (P < 0.05). CO and BSFO upregulated liver delta4 fads relative to LO, and CO further increased elovl4a and elovl5 under DHA inclusion (P < 0.05), whereas desaturase proteins remained unchanged among oils (P > 0.05). CO lowered liver TG levels and upregulated monoacylglycerol lipase (mgl) against LO and BSFO, and elevated acetyl-CoA carboxylase 1 (acc1) and fatty acid synthase (fasn) expression under DHA-inclusion (P < 0.05). Dietary DHA improved growth and tissue DHA levels, and reduced liver crude lipid and TG levels (P < 0.05), while downregulating fads2, delta4 fads, fads6 and Δ5/Δ4 Fads protein (P < 0.05). Overall, the results are consistent with largemouth bass possessing the enzymatic capacity to convert dietary ALA into n-3 LC-PUFA. Adequate dietary ALA supported growth and DHA biosynthesis, and severe ALA deficiency upregulated part n-3 LC-PUFA biosynthesis-related genes but still resulted in reduced tissue DHA. Dietary DHA inclusion alleviated growth constraints under ALA-deficient conditions.

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