数字农科院2.0

Galactosyltransferase GhRFS6 interacting with GhOPR9 involved in defense against Verticillium wilt in cotton

文献类型： 外文期刊

作者： Chang B.;Zhao L.;Feng Z.;Wei F.;Zhang Y.;Zhang Y.;Huo P.;Cheng Y.;Zhou J.;Feng H.

作者机构：

关键词： Cotton;Disease resistances;Galactosyltransferase;JA pathway;Verticillium dahliae

期刊名称： Plant Science

ISSN： 0168-9452

年卷期： 2023 年 328 卷

页码：

收录情况： SCIE（2023版）

摘要： The soil-borne fungus Verticillium dahliae causes Verticillium wilt (VW), one of the most devastating diseases of cotton. In a previous study showed that GhOPR9 played a positive role in resistance of cotton to VW through the regulation of the Jasmonic acid (JA) pathway. Furtherly, we also found that GhOPR9 interacted with a sucrose galactosyltransferase GhRFS6. Raffinose synthase (RFS) plays a key role in plant innate immunity, including the abiotic stress of drought, darkness. However, there were few reports on the effects of RFS on biotic stress. In this study, we verified the function of GhRFS6 to VW. The expression analysis showed that the GhRFS6 may be regulated by various stresses, and it was upregulated under Vd076 and Vd991 pressures. Inhibition of GhRFS6 expression, hydrogen peroxide (H2O2) content, lignin content, cell wall thickness and a series of defense responses were decreased, and the resistance of cotton to V. dahliae was decreased. In addition, this study showed that GhRFS6 has glycosyltransferase activity and can participate in the regulation of α-galactosidase activity and raffinose and inositol synthesis. And that galactose was accumulated in cotton roots after GhRFS6 silencing, which is beneficial for the colonization and growth of V. dahliae. Furthermore, overexpression of GhRFS6 in Arabidopsis thaliana enhanced plant resistance to V. dahliae. In GUS staining, the promoter expression position of GhRFS6 was also altered after V. dahliae infection. Meanwhile, GhRFS6 has also been shown to resist VW through the regulation of the JA pathway. These results suggest that GhRFS6 is a potential molecular target for improving cotton resistance to VW. © 2023 Elsevier B.V.

分类号：

  • 相关文献

[1]Analysis of sea-island cotton and upland cotton in response to Verticillium dahliae infection by RNA sequencing. Quan Sun,Huaizhong Jiang,Xiaoyan Zhu,Weina Wang,Xiaohong He,Yuzhen Shi,Youlu Yuan,Xiongming Du,Yingfan Cai. 2013

[2]The Conservation of Long Intergenic Non-Coding RNAs and Their Response to Verticillium dahliae Infection in Cotton. Chen, Li,Shen, Enhui,Zhao, Yunlei,Wang, Hongmei,Wilson, Iain,Zhu, Qian-Hao. 2022

[3]Editorial: Identification and functional dissection of stress-responsive genes in cotton. Wu J.,Wang P.,Ge X.. 2022

[4]miR398b negatively regulates cotton immune responses to Verticillium dahliae via multiple targets. Miao, Yuhuan,Chen, Kun,Deng, Jinwu,Zhang, Lin,Wang, Weiran,Kong, Jie,Klosterman, Steven J.,Zhang, Xianlong,Aierxi, Alifu,Zhu, Longfu. 2022

[5]Interactions between Verticillium dahliae and cotton: pathogenic mechanism and cotton resistance mechanism to Verticillium wilt. Zhu, Yutao,Zhao, Mei,Li, Taotao,Wang, Lianzhe,Liao, Chunli,Liu, Dongxiao,Zhang, Huamin,Zhao, Yanpeng,Liu, Lisen,Ge, Xiaoyang,Li, Bingbing. 2023

[6]A review of the pathogenicity mechanism of Verticillium dahliae in cotton. Yalin Zhang,Jinglong Zhou,Lihong Zhao,Zili Feng,Feng Wei,Hongyan Bai,Hongjie Feng,Heqin Zhu. 2022

[7]Heat Shock Transcription Factor GhHSFB2a Is Crucial for Cotton Resistance to Verticillium dahliae. Lu Liu,Qi Wang,Linfeng Zhu,Huiming Guo,Hongmei Cheng,Xiaofeng Su. 2023

[8]Acetylation of GhCaM7 enhances cotton resistance to Verticillium dahliae. Zhang, Lei,Wu, Yajie,Yu, Yongang,Zhang, Yihao,Wei, Feng,Zhu, Qian-Hao,Zhou, Jinglong,Zhao, Lihong,Zhang, Yalin,Feng, Zili,Feng, Hongjie,Sun, Jie. 2023

[9]The glycoside hydrolase 28 member VdEPG1 is a virulence factor of Verticillium dahliae and interacts with the jasmonic acid pathway-related gene GhOPR9. Liu, Shichao,Liu, Ruibing,Lv, Junyuan,Feng, Zili,Wei, Feng,Zhao, Lihong,Zhang, Yalin,Zhu, Heqin,Feng, Hongjie. 2023

[10]High-quality genome assembly of Verticillium dahliae VD991 allows for screening and validation of pathogenic genes. Yang, Jiaxiang,Liu, Lisen,Yang, Lan,Liu, Renju,Gao, Chenxu,Hu, Wei,Yan, Qingdi,Yang, Zhaoen,Fan, Liqiang. 2023

[11]Pathogenicity-related long non-coding natural antisense transcripts in Verticillium dahliae during infections in cotton. Li, Ran,Xue, Huishan,Ma, Xiyue,Zhu, He,Li, Yue,Dai, Xiaofeng,Chen, Jie-yin. 2023

[12]VdPT1 Encoding a Neutral Trehalase of Verticillium dahliae Is Required for Growth and Virulence of the Pathogen. Chen L.,Ma X.,Sun T.,Zhu Q.-H.,Feng H.,Li Y.,Liu F.,Zhang X.,Sun J.,Li Y.. 2024

[13]Suppression of plant immunity by Verticillium dahliae effector Vd6317 through AtNAC53 association. Liu, Lisen,Li, Jianing,Wang, Zhaohan,Zhou, Haodan,Wang, Ye,Qin, Wenqiang,Duan, Hongying,Zhao, Hang,Ge, Xiaoyang. 2024

[14]Verticillium dahliae effector Vd06254 disrupts cotton defence response by interfering with GhMYC3-GhCCD8-mediated hormonal crosstalk between jasmonic acid and strigolactones. Ma, Jianhui,Jiang, Fan,Yu, Yan,Zhou, Haodan,Zhan, Jingjing,Li, Jianing,Chen, Yanli,Wang, Ye,Duan, Hongying,Ge, Xiaoyang,Xu, Zhenzhen,Zhao, Hang,Liu, Lisen. 2025

[15]In the coevolution of cotton and pathogenic fungi, resistant cotton varieties lead to an escalation in the virulence of Verticillium dahliae. Yalin Zhang,Lifang Cui,Ruibing Liu,Zili Feng,Hongjie Feng,Jinglong Zhou,Lihong Zhao,Feng Wei,Heqin Zhu. 2025

[16]Deciphering the transcriptional landscape of NLR genes responding to Verticillium dahliae infection in cotton (Gossypium hirsutm L.). Shen, Enhui,Wang, Ying,Song, Jingyu,Yuan, Yuman,Li, Wei,Ye, Chuyu,Wilson, Iain W.,Fan, Longjiang,Zhao, Tianlun,Zhu, Qian-Hao. 2026

[17]Ghr-miR166b-GhHB14 Pair With GhDLO1 Partner Mediates Cotton Plant Defence Against Verticillium dahliae. Meng, Yuan,Zhao, Ge,Tang, Ye,Xu, Lin,Li, Dongliang,Ge, Xiaoyang,Wu, Jiahe. 2025

[18]Cloning and Expression of the Chloroplast Copper/Zinc-Superoxide Dismutase Gene in Upland Cotton （Gossypium hirsutum L.）. Yu Shuxun,Fan Shuli,Song Meizhen. 2007

[19]2015年全国棉花种植品种监测报告——播种品种(系)344个;数量减少83个;减幅19.4%. 毛树春,冯璐,芦建华. 2016

[20]2015年中国与美国棉花种植品种比较. 冯璐,毛树春. 2016

作者其他论文 更多>>