数字农科院2.0

GCC box in Arabidopsis PDF1.2 promoter is an essential and sufficient cis-acting element in response to MeJA treatment

文献类型： 外文期刊

作者： Zhang, HW;Xie, BY;Lu, XY;Yang, YH;Huang, RF

作者机构：

关键词： Agroinfiltation;GCC box;MeJA;PDF1.2 promoter;cis-acting element

期刊名称： CHINESE SCIENCE BULLETIN

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收录情况： SCI

摘要： The expression of Arabidopsis PDF1.2 gene is regelated by jasmonic add (JA) and ethylene (ET). It also has been well documented that GCC box is an dement responsive to ET, however, the responsive mechanism of JA in such defense gene expression is unclear. In this paper, the authors define the essential eisaeting element in PDF1.2 promoter responsive to methyl jasmonate (MeJA) throughfragment deletions and site-directed mutageneses combining Agrobacterium-medinted transient reporter gene expression in tobacco leaves. Firstly, the MeJA indncible expression of PDF1.2 was confirmed by using the upstream -1.86 kb fragment of PDF 1.2 gene. Secondly, the upstream -300- 243 bp fragment of the promoter was evidenced to respond to MeJA. To future characterize this promoter region, three point mutations were introduced into the -300--243 bp of the promoter. This result showed that the mutation of GCC box abolished MeJA induction, whereas the mutations of the G box-like the imperfect palindrome sequence did not significantly decrease MeJA indncible effect, indicating that GCC box in PDF1.2 is for MeJA induction. The sufficient responsiveness to MeJA of this GCC box was further investigated by 4xGCC fused upstream to the CaMV 35S minimal promoter. This result suggested that the fused promoter was able to activate reporter expression In response to MeJA. Thus these results indicate that the GCC box in PDF1.2 is an essential and sufficient element to confer MeJA induction.

分类号： N1

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