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Genome-Wide Analysis Of Differentially Expressed G.enes And The Modulation O f Pedv Infection In Vero E6 Cells

文献类型： 外文期刊

作者： Zhang, HW; Liu, QF; Su, WW; Wang, JK; Sun, YR; Zhang, JF; Shang, K; Chen, ZH; Cheng, SP; Wu, H

作者机构：

关键词： PEDV; RNA-Seq; mTOR signaling pathway; Antiviral

期刊名称： MICROBIAL PATHOGENESIS

ISSN： 0882-4010

年卷期： 2018 年 117 卷

页码：

收录情况： JCR（2021版）

摘要： PEDV remains one of the most important swine diseases that infects pigs of all ages. It causes devastating viral enteric disease in piglets with a high mortality rate, leading to significant threats and huge economic loss to the pork industry. In this study, a transcriptomic shotgun sequencing (RNA-Seq) procedure was used to study gene responses against PEDV infection. Genome-wide analysis of differentially expressed genes (DEGs) was performed in Vero E6 cells post-PEDV infection. mTOR signaling pathway activator-MHY1485, and inhibitor-PP242 were used to study the antiviral function. Results revealed that the IRF3 was significantly up-regulated post-PEDV infection. Although most of the IFN-regulatory and -related genes evaluated in this study were either down regulated or remained unchanged, IL11 behaved significantly up-regulated, with the peak at 16 hpi. Nearly 90% of PEDV infections were suppressed in the PP242 pretreated cells whereas the reverse effect was observed in the MYH1485 pretreated cells. Results indicated that the mTOR signaling pathway played a vital role in the PEDV antiviral regulation in the Vero E6 cells. Future studies will contribute to better understand the cellular antiviral mechanism against PEDV.

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