数字农科院2.0

Identification Of Linear B-Cell Epitopes On Hemagglutinin Protein Of Canine Distemper Virus Using Two Monoclonal Antibodies

文献类型： 外文期刊

作者： Shi, PF; Cao, ZG; Cheng, YN; Cheng, SP; Yi, L

作者机构：

关键词： canine distemper virus; hemagglutinin protein; monoclonal antibody; B-cell epitope; conservation analysis

期刊名称： FRONTIERS IN VETERINARY SCIENCE

ISSN：

年卷期： 2020 年 7 卷

页码：

收录情况： JCR（2021版）

摘要： Canine distemper virus (CDV) belongs to the Morbillivirus genus of the Paramyxoviridae family, which causes a threat to the domestic dog and fur-animal industry. Hemagglutinin protein is a major membrane protein of the vital molecular factor in CDV tropism, also known to induce hosts to produce neutralizing antibodies. In the current study, we prepared two monoclonal antibodies, 1A5 and 2B8, against the H protein of the CDV-PS strain. A series of partially overlapping synthetic peptides covering the hemagglutinin protein (amino acids 50-204) were screened to define the linear epitope identified by 1A5 and 2B8 mAbs. (120)QKTNFFNPNREFDFR(134) (F8) and (178)ARGDIFPPY(186) (F14-1) are minimal linear epitopes recognized by 1A5 and 2B8 mAbs, respectively. Further investigations revealed that F8 is conserved in different CDV strains; however, F14-1 contains mutant residues 178, 179, and 180. The epitopes F8 and F14-1 localized at the surface of hemagglutinin protein in a three-dimensional (3D) structure. CDV-infected dog serum can also recognize the identified B-cell epitopes.

分类号：

  • 相关文献

[1]Identification Of A Novel Linear B.-Cell Epitope Using A M onoclonal Antibody Against The Carboxy Terminus Of The Canine Distemper Virus Nucleoprotein And Sequence Analysis Of The Identified Epitope In. Yi, L, Cao, ZG, Tong, MW, Cheng, YN, Yang, Y, Li, S, Wang, JK, Lin, P, Sun, YR, Zhang, M, Cheng, SP. 2017

[2]Identification And Characterization Of A N.ovel B-Cell Epitope On A leutian Mink Disease Virus Capsid Protein Vp2 Using A Monoclonal Antibody. Lu, TF, Wang, YZ, Ge, JW, Ma, Q, Yan, WZ, Zhang, YY, Zhao, LL, Chen, HY. 2018

[3]Mink SLAM V-Region V74I Substitutions Contribute to the Formation of Syncytia Induced by Canine Distemper Virus. Yawen Wang,Jie Chen,Bo Hu,Chengyan Gong,Ning Shi,Mengjia Liu,Xijun Yan,Xue Bai,Jianjun Zhao. 2021

[4]Identification Of Linear B-Cell Epitopes O.n The Phosphoprotein Of C anine Distemper Virus Using Four Monoclonal Antibodies. Li, S, Yi, L, Cao, ZG, Cheng, YN, Tong, MW, Wang, JK, Lin, PK, Cheng, SP. 2018

[5]Preparation And Application Of Monoclonal A.ntibody To Sugarcane (Saccharum L . Spp. Hybrids) Sop5Cs Protein. Li, Y. R.,Wang, L. R.,Yang, L. T.,Xing, Y. X.,Huang, X.,Li, Y. R.,Huang, X.,Li, Y. R.,Li, J.,Huang, X.. 2019

[6]Evaluation Of The Protective Immunity Of Riemerella Anatipestifer Ompa. Xu, XX, Xu, YH, Miao, S, Jiang, P, Cui, JS, Gong, YS, Tan, PP, Du, XL, Islam, N, Hu, QH. 2020

[7]Development And Detection Application Of M.onoclonal Antibodies Against Zucchini Y ellow Mosaic Virus. Zhou Xue-ping,Zhou Xue-ping,Zhang Ming-hao,Chen Zhe,Wu Jian-xiang. 2017

[8]Development Of An Indirect Competitive E.lisa For The Detection O f Acenaphthene And Pyrene. Qiao, B, Li, YS, Meng, XY, Sun, Y, Hu, P, Lu, SY, Ren, HL, Liu, ZS, Zhou, Y. 2017

[9]Determination Of Deoxynivalenol By Elisa A.nd Immunochromatographic Strip Assay B ased On Monoclonal Antibodies. Yon, TT, Zhang, Q, Wang, D, Li, PW, Tang, XQ, Zhang, W. 2019

[10]Expression And Characterization Of A R.ecombinant Porcinized Antibody Against T he E2 Protein Of Classical Swine Fever Virus. Chen, SC, Li, S, Sun, HM, Li, YF, Ji, SW, Song, K, Zhang, LK, Luo, YZ, Sun, Y, Ma, JF, Liu, PH, Qiu, HJ. 2018

[11]Neutralization Of Genotype 2 Porcine E.pidemic Diarrhea Virus Strains B y A Novel Monoclonal Antibody. Liu, JB, Shi, HY, Chen, JF, Zhang, X, Ji, ZY, Yuan, J, Shi, D, Cao, LY, Zhu, XD, Dong, H, Wang, XB, Zhang, JL, Feng, L. 2017

[12]An Ultrasensitive Gray-Imaging-Based Quantitative Immunochromatographic D.etection Method For Fumonisin B -1 In Agricultural Products. Tang, XQ, Li, PW, Zhang, ZW, Zhang, Q, Guo, JN, Zhang, W. 2017

[13]Identification Of A Serotype-Independent Linear E.pitope Of Foot-And-Mouth Disease V irus. Yang, BL, Wang, MX, Liu, WM, Xu, ZQ, Wang, HW, Yang, DC, Ma, WG, Zhou, GH, Yu, L. 2017

[14]Identification Of A Conformational Neutralizing E.pitope On The Vp1 P rotein Of Type A Foot-And-Mouth Disease Virus. Liu, WM, Yang, BL, Wang, MX, Wang, HW, Yang, DC, Ma, WG, Zhou, GH, Yu, L. 2017

[15]Monoclonal Antibody-Based Serological Detection Methods F.or Wheat Dwarf Virus. Zhang, MH, Chen, R, Zhou, XP, Wu, JX. 2018

[16]The Simultaneous Detection of Multiple Antibiotics in Milk and Pork Based on an Antibody Chip Biosensor. Xiao J., Wei N., Wu S., Li H., Yin X., Si Y., Li L., Peng D.. 2022

[17]Characterization Of Monoclonal Antibodies Against S.igma A Protein And C ross-Reactive Epitope Identification And Application For Detection Of Duck And Chicken Reovirus Infections. Chen, XM, Li, TT, Chen, XD, Li, CX, Lin, WW, Liu, HY, Song, SP, Bai, XF, Zhang, Y. 2019

[18]Characterization Of A Novel Interleukin-1 Receptor Antagonist From Sheep (Ovis Aries). Zhang, SJ, Guo, X, Hu, P, Lu, SY, Liu, NN, Fu, BQ, Wang, N, Li, YS, Wang, LL, Chang, J, Chang, HZ, Liu, ZS, Zhou, Y, Ren, HL. 2020

[19]Targeting The Gram-Negative Bacteria Peptidoglycan S.ynthase Mray As A N ew Approach For Monoclonal Antibody Anti-Bacterial Activity. Cao, J, Yi, F, Tian, QF, Dang, GH, Si, W, Liu, SG, Yu, SY. 2017

[20]Identification Of A Novel B-Cell Epitope In The Spike Protein Of Porcine Epidemic Diarrhea Virus. Kong, N, Meng, Q, Jiao, YJ, Wu, YG, Zuo, YW, Wang, H, Sun, DG, Dong, SJ, Zhai, HJ, Tong, W, Zheng, H, Yu, H, Tong, GZ, Xu, YJ, Shan, TL. 2020

作者其他论文 更多>>