数字农科院2.0

Efficient induction and rapid identification of haploid grains in tetraploid wheat by editing genes TtMTL and pyramiding anthocyanin markers

文献类型： 外文期刊

作者： Yanan Chang;Huali Tang;Surong Wang;Xi Li;Peipei Huang;Jiahui Zhang;Ke Wang;Yueming Yan;Xingguo Ye

作者机构：

关键词： CRISPR/Cas9;durum wheat;haploid induction;purple embryo;TtMTL genes

期刊名称： Frontiers in Plant Science

ISSN： 1664-462X

年卷期： 2024 年 15 卷

页码：

收录情况： SCIE（2024版）

摘要： Doubled haploid (DH) technology provides an effective way to generate homozygous genetic and breeding materials over a short period of time. We produced three types of homozygous TtMTL gene-edited mutants (mtl-a, mtl-b, and mtl-ab) by CRISPR/Cas9 in durum wheat. PCR restriction enzymes and sequencing confirmed that the editing efficiency was up to 53.5%. The seed-setting rates of the three types of mutants ranged from 20% to 60%. Abnormal grain phenotypes of kernel, embryo, and both embryo and endosperm abortions were observed in the progenies of the mutants. The average frequency of embryo-less grains was 25.3%. Chromosome counting, guard cell length, and flow cytometry confirmed that the haploid induction rate was in the range of 3%–21% in the cross- and self-pollinated progenies of the mtl mutants (mtl-a and mtl-ab). Furthermore, we co-transformed two vectors, pCRISPR/Cas9-MTL and pBD68-(ZmR + ZmC1), into durum wheat, to pyramide Ttmtl-edited mutations and embryo-specifically expressed anthocyanin markers, and developed a homozygous durum haploid inducer with purple embryo (DHIPE). Using DHIPE as the male parent to be crossed with the wild-type Kronos, the grains with white embryos were identified as haploid, while the grains with purple embryos were diploid. These findings will promote the breeding of new tetraploid wheat varieties.

分类号：

  • 相关文献

[1]Identification and Microsatellite Markers of a Resistance Gene to Powdery Mildew in Common Wheat Introgressed from Triticum durum. Zhu Zhendong,Kong Xiuying,Zhou Ronghua,Jia Jizeng. 2004

[2]Physical mapping of puroindoline b-2 genes and molecular characterization of a novel variant in durum wheat (Triticum turgidum L.). Chen, F.,Xu, H. -X.,Zhang, F. -Y.,Dong, Z. -D.,Zhan, K. -H.,Cheng, X. -Y.,Cui, D. -Q.,Xia, X. -C.,He, Z. -H.,He, Z. -H.,Wang, D. -W.. 2011

[3]Genetic dissection of seminal root architecture in elite durum wheat germplasm. Sanguineti, M. C.,Li, S.,Maccaferri, M.,Corneti, S.,Rotondo, F.,Chiari, T.,Tuberosa, R.. 2007

[4]Identification and Microsatellite Markers of a Resistance Gene to Powdery Mildew in Common Wheat Introgressed from Triticum durum. Zhu, ZD,Kong, XY,Zhou, RH,Jia, JZ.

[5]Characterization and mapping of novel chlorophyll deficient mutant genes in durum wheat. Li, Ning,Jia, Jizeng,Xia, Chuan,Liu, Xu,Kong, Xiuying.

[6]Matrilineal empowers wheat pollen with haploid induction potency by triggering postmitosis reactive oxygen species activity. Sun, Guoliang,Geng, Shuaifeng,Zhang, Hongjie,Jia, Meiling,Wang, Zhenyu,Deng, Zhongyin,Tao, Shu,Liao, Ruyi,Wang, Fang,Kong, Xingchen,Fu, Mingxue,Liu, Shaoshuai,Li, Aili,Mao, Long. 2022

[7]Genome edited wheat- current advances for the second green revolution. Muhammad Jawad Akbar Awan,Komal Pervaiz,Awais Rasheed,Imran Amin,Nasir A. Saeed,Kanwarpal S. Dhugga,Shahid Mansoor. 2022

[8]Editorial: Applications of fast breeding technologies in crop improvement and functional genomics study. Xingguo Ye,Fangpu Han. 2024

[9]Harnessing haploid-inducer mediated genome editing for accelerated maize variety development. Li, Lina,Fu, Xiao,Qi, Xiantao,Xiao, Bing,Liu, Changling,Wu, Qingyu,Zhu, Jinjie,Xie, Chuanxiao. 2025

[10]Exploring potential strategies for haploid induction based on double fertilization in plants. Li, Tengyu,Wang, Chenlei,Pan, Jingwen,Tabusam, Javaria,Li, Yan,Yao, Jinbo,Chen, Wei,Wang, Yazhong,Gao, Wei,Rong, Junkang,Ahmad, Zeeshan,Houben, Andreas,Zhu, Shouhong,Jin, Shuangxia,Zhang, Yongshan. 2025

[11]Advances in doubled haploid technology for rice breeding: mechanisms, applications, and future perspectives. Lin, Yiling,Mao, Fangming,Huang, Yuheng,Huang, Derun,Wang, Kejian,Yan, Song,Liu, Chaolei. 2026

[12]CRISPR/Cas9系统介导的植物基因组编辑及其应用. 王红霞,张一卉,李景娟,贺立龙,高建伟. 2018

[13]利用CRISPR/Cas9技术对燕麦乙酰辅酶A羧化酶(ACCase)基因的编辑. 于东洋,王凤梧,融晓萍,武志娟,王鑫,杨燕,韩冰,田青松. 2019

[14]CRISPR/Cas9技术在植物基因功能研究和新种质创制中的应用与展望. 周焕斌. 2016

[15]CRISPR/Cas9系统介导基因组编辑的研究进展. 刘志国. 2014

[16]利用CRISPR/Cas9技术敲除水稻Pi21基因的效率分析. 范方军,李文奇,朱金燕,王军. 2016

[17]CRISPR/Cas9介导基因组编辑技术在植物基因中的研究进展. 胡颂平,吴云花,邹国兴,聂元元,杜鹃. 2016

[18]利用CRISPR/Cas9系统敲除绵羊皮肤成纤维细胞Wnt2基因的研究. 冯新宇,袁超,郭婷婷,刘建斌,郭健,牛春娥,孙晓萍,冯瑞林,岳耀敬,杨博辉. 2018

[19]弓形虫Rop46基因敲除虫株的构建及其生长复制能力评价. 王成双,马秀英,郑君,贾洪林,王新. 2016

[20]CRISPR/Cas9技术在植物基因功能研究和新种质创制中的应用与展望. 周焕斌. 2016

作者其他论文 更多>>