数字农科院2.0

Insights into non-isoflavone flavonoid distribution and variation across soybean germplasm using HPLC-MS/MS

文献类型： 外文期刊

作者： Iqbal A;Zhang S; Khattak A N;Tazeb A;Hou Y;Ahsan M;Sie E K; Li J; Li B;Sun J

关键词： Soybean (Glycine max L.); Non-isoflavone flavonoids; HPLC-MS/MS; Natural variation

期刊名称： LWT-FOOD SCIENCE AND TECHNOLOGY

ISSN： 0023-6438

年卷期： 2025 年

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Flavonoids are key secondary metabolites in soybean, contributing to the plant defense mechanism and human health. The isoflavones in soybean have been well studied, and non-isoflavone flavonoids remain unexplored. This study optimized and validated a highly sensitive high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS/MS) method to quantify 13 flavonoids from five subclasses (chalcone, flavanols, flavanones, flavones, and flavonols), representing 82 % of total flavonoid content (TFC) in soybean seeds. Profiling 711 diverse soybean accessions from China’s three main ecoregions revealed significant variation, with TFC ranging from 667 to 24503 μg/100g, representing a 36-fold difference; four accessions exceeding 20000 μg/100g. The Huang Huai Hai Valley Region (HR) had the highest mean TFC (3341 μg/100g). Black seed coat accessions exhibited the highest flavonoid concentrations. The cumulative share of four major flavonoids, epicatechin, quercetin 3-galactoside, prunin, and apigenin, accounted for 77–81 % of TFC, particularly the darkpigmented seedcoat enriched with epicatechin and quercetin derivatives. TFC correlated positively with most flavonoids, isoflavones, and protein, but negatively with oil content. Major flavonoid components were negatively associated with longitude, suggesting that geographical origin affects flavonoid accumulation. The findings provide actionable insights for breeding nutritionally enriched soybean varieties, with implications for functional food development.

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