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ClpP protease modulates bacterial growth, stress response, and bacterial virulence in Brucella abortus

文献类型： 外文期刊

作者： Dongjie Sun;Yufu Liu;Xiaowei Peng;Hao Dong;Hui Jiang;Xuezheng Fan;Yu Feng;Jiali Sun;Kun Han;Qiang Gao;Jianrui Niu;Jiabo Ding

作者机构：

关键词： Brucella;ClpP protease;iTRAQ analysis;RNA-seq;virulence

期刊名称： Veterinary research

ISSN： 1297-9716

年卷期： 2023 年 54 卷 1 期

页码：

收录情况： SCIE（2023版）

摘要： The process of intracellular proteolysis through ATP-dependent proteases is a biologically conserved phenomenon. The stress responses and bacterial virulence of various pathogenic bacteria are associated with the ATP-dependent Clp protease. In this study, a Brucella abortus 2308 strain, ΔclpP, was constructed to characterize the function of ClpP peptidase. The growth of the ΔclpP mutant strain was significantly impaired in the TSB medium. The results showed that the ΔclpP mutant was sensitive to acidic pH stress, oxidative stress, high temperature, detergents, high osmotic environment, and iron deficient environment. Additionally, the deletion of clpP significantly affected Brucella virulence in macrophage and mouse infection models. Integrated transcriptomic and proteomic analyses of the ΔclpP strain showed that 1965 genes were significantly affected at the mRNA and/or protein levels. The RNA-seq analysis indicated that the ΔclpP strain exhibited distinct gene expression patterns related to energy production and conversion, cell wall/membrane/envelope biogenesis, carbohydrate transport, and metabolism. The iTRAQ analysis revealed that the differentially expressed proteins primarily participated in amino acid transport and metabolism, energy production and conversion, and secondary metabolites biosynthesis, transport and catabolism. This study provided insights into the preliminary molecular mechanism between Clp protease to bacterial growth, stress response, and bacterial virulence in Brucella strains.

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