数字农科院2.0

Molecular Characterization of Chemosensory Protein (CSP) Genes and the Involvement of AgifCSP5 in the Perception of Host Location in the Aphid Parasitoid Aphidius gifuensis

文献类型： 外文期刊

作者： Jun Jiang;Jiayi Xue;Miaomiao Yu;Xin Jiang;Yumeng Cheng;Huijuan Wang;Yanxia Liu;Wei Dou;Jia Fan;Julian Chen

作者机构：

关键词： Aphidius gifuensis;behavioral response;binding affinity;chemosensory protein;molecular docking;tissue expression profile

期刊名称： International Journal of Molecular Sciences

ISSN： 1661-6596

年卷期： 2024 年 25 卷 12 期

页码：

收录情况： SCIE（2024版）

摘要： Aphidius gifuensis is the dominant parasitic natural enemy of aphids. Elucidating the molecular mechanism of host recognition of A. gifuensis would improve its biological control effect. Chemosensory proteins (CSPs) play a crucial role in insect olfactory systems and are mainly involved in host localization. In this study, a total of nine CSPs of A. gifuensis with complete open reading frames were identified based on antennal transcriptome data. Phylogenetic analysis revealed that AgifCSPs were mainly clustered into three subgroups (AgifCSP1/2/7/8, AgifCSP3/9, and AgifCSP4/5/6). AgifCSP2/5 showed high expression in the antennae of both sexes. Moreover, AgifCSP5 was found to be specifically expressed in the antennae. In addition, fluorescent binding assays revealed that AifCSP5 had greater affinities for 7 of 32 volatile odor molecules from various sources. Molecular docking and site-directed mutagenesis results revealed that the residue at which AgifCSP5 binds to these seven plant volatiles is Tyr75. Behavior tests further confirmed that trans-2-nonenal, one of the seven active volatiles in the ligand binding test, significantly attracted female adults at a relatively low concentration of 10 mg/mL. In conclusion, AgifCSP5 may be involved in locating aphid-infested crops from long distances by detecting and binding trans-2-nonenal. These findings provide a theoretical foundation for further understanding the olfactory recognition mechanisms and indirect aphid localization behavior of A. gifuensis from long distances by first identifying the host plant of aphids.

分类号：

  • 相关文献

[1]Expression Profiles and Functional Characterization of Chemosensory Protein 15 (HhalCSP15) in the Brown Marmorated Stink Bug Halyomorpha halys. Zehua Wang,Fan Yang,Ang Sun,Shuang Shan,Yongjun Zhang,Shanning Wang. 2021

[2]Characterization of the chemosensory protein EforCSP3 and its potential involvement in host location by Encarsia formosa. WANG K.,HE Y.-Y.,ZHANG Y.-J.,GUO Z.-J.,XIE W.,WU Q.-J.,WANG S.-L.. 2023

[3]Identification and characterization of the distinct expression profiles of candidate chemosensory membrane proteins in the antennal transcriptome of Adelphocoris lineolatus (Goeze). Ma, X. -Y.,Liu, Z. -W.,Xiao, Y.,Sun, L.,Ma, X. -Y.,Dong, K.,Liu, H. -W.,Wang, Q.,Guo, Y. -Y.,Zhang, Y. -J.,Sun, L..

[4]Cloning and analysis of DnaJ family members in the silkworm, Bombyx mori. Li, Yinu,Bu, Cuiyu,Li, Tiantian,Wang, Shibao,Jiang, Feng,Yang, Huipeng,Zhang, Zhifang,Yi, Yongzhu.

[5]Toxicity and binding analyses of Bacillus thuringiensis toxin Vip3A in Cry1Ac-resistant and -susceptible strains of Helicoverpa armigera (Hubner). Lu Qiong,Zhang Yan,Liang Ge-mei,Zhang Qian,Chen Li-zhen. 2015

[6]Molecular cloning, expression profile, odorant affinity, and stability of two odorant-binding proteins in Macrocentrus cingulum Brischke (Hymenoptera: Braconidae). Zhang, Tiantao,Wang, Zhenying,He, Kanglai,Bai, Shuxiong,Ahmed, Tofael.

[7]New insights on the role of alkaline phosphatase 2 from Spodoptera exigua (Hubner) in the action mechanism of Bt toxin Cry2Aa. Zhao, Man,Wei, Jizhen,Zhang, Wanna,Wang, Bingjie,Khaing, Myint Myint,Liang, Gemei.

[8]Comparative Transcriptome And Itraq Proteome A.nalyses Reveal The Mechanisms O f Diapause In Aphidius Gifuensis Ashmead (Hymenoptera: Aphidiidae). Hong-Zhi Zhang ,Yu-Yan Li , Tao An , Feng-Xia Huang, Meng-Qing Wang , Chen-Xi Liu , Jian-Jun Mao,Li-Sheng Zhang. 2018

[9]Molecular Cloning, Expression Profile, Odorant A.ffinity, And Stability Of T wo Odorant-Binding Proteins In Macrocentrus Cingulum Brischke (Hymenoptera: Braconidae). Ahmed, Tofael,Wang, Zhenying,Zhang, Tiantao,Ahmed, Tofael,Bai, Shuxiong,He, Kanglai. 2017

[10]Gene cloning and expression analysis of trehalose-6-phosphate synthase, glycogen synthase and glycogen phosphorylase reveal the glycometabolism in the diapause process of Aphidius gifuensis. Li, Yu Yan,Wang, Meng Qing,Xie, Ying Qiang,Li, Ping,Zhang, Hong Zhi,Zhang, Li Sheng,Xiang, Mei. 2020

[11]Plant-Mediated Interactions Between Two Cereal A.phid Species: Promotion Of A phid Performance And Attraction Of More Parasitoids By Infestation Of Wheat With Phytotoxic Aphid Schizaphis Graminum. Zhang, Y,Fan, J,Fu, Y,Francis, F,Chen, JL. 2019

[12]The Potential Coordination Of The H.eat-Shock Proteins And Antioxidant E nzyme Genes Of Aphidius Gifuensis In Response To Thermal Stress. Yu, Wen-Bo,Zhang, Shi-Ze,Liu, Tong-Xian,Kang, Zhi-Wei,Kang, Zhi-Wei,Tan, Xiao-Ling,Liu, Xiang,Liu, Fang-Hua,Tian, Hong-Gang. 2017

[13]Differential Expression Analysis Of Olfactory G.enes Based On A C ombination Of Sequencing Platforms And Behavioral Investigations In Aphidius Gifuensis. Xu, Qingxuan,Zhang, Qian,Xue, Wenxin,Fan, Jia,Chen, Julian,Sun, Jingrui,Han, Zongli. 2018

[14]Differential Expression Analysis of Olfactory Genes Based on A Combination of Sequencing Platforms and Behavioral Investigations in Aphidius Gifuensis. Fan, J,Zhang, Q,Xu, QX,Xue, WX,Han, ZL,Sun, JR,Chen, JL. 2018

[15]Vip3Aa domain IV and V mutants confer higher insecticidal activity against Spodoptera frugiperda and Helicoverpa armigera. Yang, Xiaoxue,Wang, Zeyu,Geng, Lili,Chi, Baoyan,Liu, Rongmei,Li, Haitao,Gao, Jiguo,Zhang, Jie. 2022

[16]New insights into the pH dependence of anthocyanin-protein interactions by a case study of cyanidin-3-O-glucoside and bovine serum albumin. Peiqing Yang,Wenxin Wang,Zhenzhen Xu,Lei Rao,Liang Zhao,Yongtao Wang,Xiaojun Liao. 2023

[17]Vkorc1 polymorphisms of the Norway rats in China: Implications for rodent management and evolutionary origin of anticoagulant resistance mutations. Xiuhui Wang,Xiaohui Ma,Meng Qin,Ting Sun,Dawei Wang,Ning Li,Xiaohui Liu,Meidong Jing,Deng Wang,Ying Song. 2024

[18]Effect of acclimation on heat-escape temperatures of two aphid species: Implications for estimating behavioral response of insects to climate warming. Ma, Gang,Ma, Chun-Sen.

[19]A Combination of Olfactory and Visual Cues Enhance the Behavioral Responses of Apolygus lucorum. Pan, Hongsheng,Xiu, Chunli,Lu, Yanhui,Pan, Hongsheng.

[20]GOBP1 from the variegated cutworm peridroma saucia (Hübner) (lepidoptera: Noctuidae) displays high binding affinities to the behavioral attractant (Z)-3-hexenyl acetate. Ya Lan Sun,Jun Feng Dong,Yue Qin Song,Shao Li Wang. 2021

作者其他论文 更多>>