数字农科院2.0

A Comprehensive Transcriptomic and Proteomics Analysis of Candidate Secretory Proteins in Rose Grain Aphid, Metopolophium dirhodum (Walker)

文献类型： 外文期刊

作者： Atsbha Gebreslasie Gebrekidan;Yong Zhang;Julian Chen

作者机构：

关键词： functional annotation;salivary protein;sequence similarity;unigene

期刊名称： Current Issues in Molecular Biology

ISSN： 1467-3037

年卷期： 2025 年 46 卷 12 期

页码：

收录情况： SCIE（2024版）

摘要： The Rose grain aphid, a notable agricultural pest, releases saliva while feeding. Yet, there is a need for a comprehensive understanding of the specific identity and role of secretory proteins released during probing and feeding. Therefore, a combined transcriptomic and proteomic approach was employed in this study to identify putative secretory proteins. The transcriptomic sequencing result led to the assembly of 18,030 unigenes out of 31,344 transcripts. Among these, 705 potential secretory proteins were predicted and functionally annotated against publicly accessible protein databases. Notably, a substantial proportion of secretory genes (71.5%, 69.08%, and 60.85%) were predicted to encode known proteins in Nr, Pfam, and Swiss-Prot databases, respectively. Conversely, 27.37% and 0.99% of gene transcripts were predicted to encode known proteins with unspecified functions in the Nr and Swiss-Prot databases, respectively. Meanwhile, the proteomic analysis result identified, 15 salivary proteins. Interestingly, most salivary proteins (i.e., 60% of the proteins) showed close similarity to A. craccivora, while 46.67% showed close similarity to A. glycines, M. sacchari and S. flava. However, to verify the expression of these secretory genes and characterize the biological function of salivary proteins further investigation should be geared towards gene expression and functional analysis.

分类号：

  • 相关文献

[1]干旱胁迫下棉花根SSH文库的构建与分析. 王俊娟,宋丽艳,樊伟丽,崔宇鹏,叶武威. 2011

[2]干旱胁迫下棉花SSH文库构建及其抗旱相关基因分析. 王德龙,叶武威,王俊娟,宋丽艳,樊伟丽,崔宇鹏. 2010

[3]紫花苜蓿响应低温胁迫转录因子的鉴定及表达分析. 齐晓,闵学阳,张正社,孙启忠,刘文献. 2017

[4]Litoribacter ruber gen. nov., sp nov., an alkaliphilic, halotolerant bacterium isolated from a soda lake sediment. Tian, Shi-Ping,Wang, Yong-Xia,Hu, Bin,Xiao, Wei,Chen, Yun,Lai, Yong-Hong,Wen, Meng-Liang,Cui, Xiao-Long,Tian, Shi-Ping,Wang, Yong-Xia,Hu, Bin,Xiao, Wei,Chen, Yun,Lai, Yong-Hong,Wen, Meng-Liang,Cui, Xiao-Long,Zhang, Xiao-Xia.

[5]Molecular characteristics of the alpha- and beta-tubulin genes of Nosema philosamiae. Zhu, Feng,Shen, Zhongyuan,Xu, Li,Guo, Xijie,Shen, Zhongyuan,Xu, Li,Guo, Xijie.

[6]A collection of 10,096 indica rice full-length cDNAs reveals highly expressed sequence divergence between Oryza sativa indica and japonica subspecies. Liu, Xiaohui,Lu, Tingting,Yu, Shuliang,Li, Ying,Huang, Yuchen,Huang, Tao,Zhang, Lei,Zhu, Jingjie,Zhao, Qiang,Fan, Danlin,Mu, Jie,Shangguan, Yingying,Feng, Qi,Guan, Jianping,Ying, Kai,Zhang, Yu,Lin, Zhixin,Sun, Zongxiu,Qian, Qian,Lu, Yuping,Han, Bin.

[7]Large-scale sequencing of normalized full-length cDNA library of soybean seed at different developmental stages and analysis of the gene expression profiles based on ESTs. Sha, Ai-Hua,Li, Chen,Yan, Xiao-Hong,Shan, Zhi-Hui,Zhou, Xin-An,Jiang, Mu-Lan,Mao, Han,Chen, Bo,Wan, Xia,Wei, Wen-Hui.

[8]PeanutDB: an integrated bioinformatics web portal for Arachis hypogaea transcriptomics. Shu, Changlong,Zhang, Jie,Schmidt, Emily,Li, Pei,Lenox, Douglas,Liu, Lin,Liang, Chun,Schmidt, Emily,Lenox, Douglas,Liang, Chun. 2012

[9]Silencing an aphid-specific gene SmDSR33 for aphid control through plant-mediated RNAi in wheat. Zhang J.,Li H.,Zhong X.,Tian J.,Segers A.,Xia L.,Francis F.. 2023

[10]Salivary Effector Sm9723 of Grain Aphid Sitobion miscanthi Suppresses Plant Defense and Is Essential for Aphid Survival on Wheat. Yong Zhang,Xiaobei Liu,Yu Fu,Leonardo Crespo-Herrera,Huan Liu,Qian Wang,Yumeng Zhang,Julian Chen. 2022

[11]Activation of Wheat Defense Response by Buchnera aphidicola-Derived Small Chaperone Protein GroES in Wheat Aphid Saliva. Qian Li,Yu Fu,Xiaobei Liu,Jingxuan Sun,Maolin Hou,Yong Zhang,Julian Chen. 2022

[12]Salivary Protein Cyclin-Dependent Kinase-like from Grain Aphid Sitobion avenae Suppresses Wheat Defense Response and Enhances Aphid Adaptation. Yumeng Zhang,Xiaobei Liu,Yu Sun,Yong Liu,Yong Zhang,Tianbo Ding,Julian Chen. 2024

[13]AgoArmet and AgoC002: key effector proteins in cotton aphids host adaptation. Hui Xue,Mengjie Yan,Xiangzhen Zhu,Li Wang,Lizhen Chen,Junyu Luo,Jinjie Cui,Xueke Gao. 2024

作者其他论文 更多>>