数字农科院2.0

RNA sequencing analysis reveals the impact of Schistosoma japonicum worm on host liver fibrosis

文献类型： 外文期刊

作者： Haoran Zhong;Bowen Dong;Danlin Zhu;Zhiqiang Fu;Jinming Liu;Guiquan Guan;Yamei Jin

作者机构：

关键词： Hepatic stellate cells;Liver fibrosis;miRNA;Schistosome worm;Schistosomiasis

期刊名称： Acta Tropica

ISSN： 0001-706X

年卷期： 2025 年 270 卷

页码：

收录情况： SCIE（2025版）

摘要： Schistosomiasis, a neglected tropical disease caused by Schistosoma species, remains a significant global health issue. One of its severe complications is liver fibrosis. The role of schistosome worms in liver fibrosis has been largely understudied, with most research focusing on the effects of schistosome eggs. This study investigates the direct impact of Schistosoma japonicum worms on liver fibrosis and explores the potential molecular mechanisms, particularly the role of microRNAs (miRNAs) secreted by the worms. RNA sequencing and small RNA sequencing were employed to investigate the effects of S. japonicum worms on hepatic stellate cells (HSCs) within a Transwell co-culture system. Bioinformatic tools were utilized to predict target genes for identified S. japonicum-derived miRNAs. A mouse model was used, encompassing both bisexual and single-sex male worm infections. Immunohistochemistry and fluorescence in situ hybridization revealed that S. japonicum infection significantly upregulated fibrosis-related proteins in liver tissues, particularly in the periportal regions in the single-sex male infection group. RNA-seq analysis indicated that schistosome worms could influence the gene expression profiles of HSCs, with significant enrichment in collagen organization and fibrosis-related signaling pathways. miRNA profiling identified several Sj-miRNAs, including sja-miR-277, which was shown to inhibit HSC proliferation and activation in vitro. Co-localization of sja-miR-277 with the HSC activation marker α-SMA in liver sections confirmed the involvement of worm-derived miRNAs in HSC activation. Overall, these findings provide novel insights into the role of worm-derived molecules in schistosome-induced liver fibrosis and highlight potential targets for therapeutic intervention.

分类号：

  • 相关文献

[1]Commentary: Induction of hepatic fibrosis in mice with schistosomiasis by extracellular microRNA-30 derived from Schistosoma japonicum eggs. Haoran Zhong,Yamei Jin. 2025

[2]Multifunctional Roles of MicroRNAs in Schistosomiasis. Zhong, Haoran,Jin, Yamei. 2022

[3]Interplays of liver fibrosis-associated microRNAs: Molecular mechanisms and implications in diagnosis and therapy. Li, Hong,Liu, Tingli,Yang, Yongchun,Cho, William C.,Flynn, Robin J.,Harandi, Majid Fasihi,Song, Houhui,Luo, Xuenong,Zheng, Yadong. 2023

[4]Integrative Analysis of RNA Expression and Regulatory Networks in Mice Liver Infected by Echinococcus multilocularis. Liu, Tingli,Li, Hong,Li, Yanping,Wang, Liqun,Chen, Guoliang,Pu, Guiting,Guo, Xiaola,Cho, William C.,Fasihi Harandi, Majid,Zheng, Yadong,Luo, Xuenong. 2022

[5]Membrane proteomics and transcriptomic profiling analysis of hepatic stellate cells co-incubated with Schistosoma japonicum eggs. Bowen Dong,Haoran Zhong,Danlin Zhu,Hao Li,Ke Lu,Zhiqiang Fu,Jinming Liu,Yamei Jin. 2025

[6]Anti-Liver Fibrosis Activity and the Potential Mode of Action of Ruangan Granules: Integrated Network Pharmacology and Metabolomics. Shang, Xiaofei,Yuan, Huixin,Dai, Lixia,Liu, Yang,He, Jian,Chen, Huan,Li, Hongyan,Li, Xiuhui. 2022

[7]Lingonberry Anthocyanins Inhibit Hepatic Stellate Cell Activation and Liver Fibrosis via TGFβ/Smad/ERK Signaling Pathway. Guokun Zhang,Yunyao Jiang,Xin Liu,Yongyan Deng,Bin Wei,Liyan Shi. 2021

[8]A novel RELA K119 deacetylation mediated by SIRT7 is a pivotal activator to exacerbate liver inflammation and fibrosis in teleosts. Wu, Xiaoliang,Liang, Xiaofang,Li, Min,Liu, Jiacheng,Ge, Chunyu,Xie, Xiaoze,Wang, Jie,Zheng, Yinhua,Wang, Hao,Wu, Xiufeng,Gu, Xu,Xue, Min. 2025

[9]Schistosoma japonicum sja-let-7 Inhibits the Growth of Hepatocellular Carcinoma Cells via Cross-Species Regulation of Col1α2. Haoran Zhong,Bowen Dong,Danlin Zhu,Zhiqiang Fu,Jinming Liu,Guiquan Guan,Yamei Jin. 2024

[10]Rapid detection and identification of four major Schistosoma species by high-resolution melt (HRM) analysis. Li, Juan,Zhu, Xing-Quan,Li, Juan,Zhao, Guang-Hui,Lin, RuiQing,Blair, David,Sugiyama, Hiromu.

[11]Biotechnological advances in the diagnosis, species differentiation and phylogenetic analysis of Schistosoma spp.. Zhao, Guang-Hui,Li, Juan,Li, Xiao-Yan,Zhu, Xing-Quan,Zhao, Guang-Hui,Li, Juan,Lin, Rui-Qing,Blair, David,Li, Xiao-Yan,Elsheikha, Hany M.,Zou, Feng-Cai,Zhu, Xing-Quan. 2012

[12]Prevention of Schistosoma japonicum infection in mice with long-acting praziquantel implants. Lei, Lei,Guo, Shengrong,Cheng, Liang,Hou, Jingwen,Zhu, Chuangang,Shi, Yaojun,Jiang, Yan,Lin, Jiaojiao. 2012

[13]Role of microRNAs in schistosomes and schistosomiasis. Zhu, Lihui,Liu, Jinming,Cheng, Guofeng. 2014

[14]In vitro and in vivo evaluation of small interference RNA-mediated gynaecophoral canal protein silencing in Schistosoma japonicum. Cheng, Guofeng,Fu, Zhiqiang,Lin, Jiaojiao,Cai, Youmin,Shi, Yi,Zhou, Yuancong,Jin, Youxin. 2009

[15]MicroRNAs: Potentially important regulators for schistosome development and therapeutic targets against schistosomiasis. Cheng, Guofeng,Jin, Youxin,Jin, Youxin. 2012

[16]A specific indel marker for the Philippines Schistosoma japonicum revealed by analysis of mitochondrial genome sequences. Li, Juan,Li, Juan,Zhu, Xing-Quan,Chen, Fen,Sugiyama, Hiromu,Blair, David,Lin, Rui-Qing.

[17]A specific PCR assay for the identification and differentiation of Schistosoma japonicum geographical isolates in mainland China based on analysis of mitochondrial genome sequences. Zhao, Guang-Hui,Li, Juan,Song, Hui-Qun,Li, Xiao-Yan,Zou, Feng-Cai,Zhu, Xing-Quan,Zhao, Guang-Hui,Zhao, Guang-Hui,Li, Juan,Song, Hui-Qun,Chen, Fen,Lin, Rui-Qing,Yuan, Zi-Guo,Weng, Ya-Biao,Li, Xiao-Yan,Zou, Feng-Cai,Zhu, Xing-Quan,Hu, Min,Zhu, Xing-Quan.

[18]Changes in microRNA expression in response to Schistosoma japonicum infection. Hong, Y.,Fu, Z.,Cao, X.,Lin, J.,Cao, X.,Lin, J..

[19]Altered levels of circulating miRNAs are associated Schistosoma japonicum infection in mice. Zhu, Lihui,Dao, Jinwei,Du, Xiaoli,Li, Hao,Lu, Ke,Liu, Jinming,Cheng, Guofeng. 2015

[20]Successful outcome of an integrated strategy for the reduction of schistosomiasis transmission in an endemically complex area. Li, Shi-Zhu,Qian, Ying-Jun,Wang, Qiang,Zhou, Xiao-Nong,Yang, Kun,Zhang, Hua-Ming,Liu, Jun,Chen, Mu-Hua,Huang, Xi-Bao,Xu, Yin-Long. 2012

作者其他论文 更多>>