数字农科院2.0

Anti-melanogenic effect and mechanism of oxypaeoniflorin derived from edible Paeonia ostii flower on melanogenesis in B16F10 cells and zebrafish

文献类型： 外文期刊

作者： Kejie Peng;Yixuan Wang;Zhangtie Wang;Jing Wang;Yuhang Zhu;Qiannan Pan;Yumeng Li;Jian Mu;Xiuxin Zhang;Baiyi Lu

作者机构：

关键词： Melanogenesis;Nrf2;Oxidative stress;Oxypaeoniflorin;Paeonia ostii flower

期刊名称： Food Bioscience

ISSN： 2212-4292

年卷期： 2025 年 73 卷

页码：

收录情况： SCIE（2025版） ; ; 农林核心（2024版）

摘要： The Paeonia ostii flower (POF), an approved novel food resource, exhibits skin-lightening properties. However, its bioactive components and mechanisms remain unclear. Among the identified constituents through UPLC-MS/MS quantification, oxypaeoniflorin, a characteristic monoterpene glycoside in POF, was prioritized for further investigation due to its potent performance in molecular docking and tyrosinase inhibition assays. Our assessments of melanin content and tyrosinase activity in both B16F10 cells and zebrafish, combined with stereomicroscopic analysis of endogenous melanin deposition, revealed that oxypaeoniflorin significantly inhibited melanin synthesis. These effects were comparable to the positive control arbutin. Mechanistic studies revealed that oxypaeoniflorin suppressed oxidative stress by lowering ROS and NADP+/NADPH ratio while regulating GSH synthesis. Downregulation of key melanogenic proteins (MC1R, MITF, TYR, TRP-1, TRP-2, PMEL) was validated by qPCR and Western blotting. Transmission electron microscopy further demonstrated impaired melanosome maturation by oxypaeoniflorin. RNA-seq identified the Nrf2 pathway as the core mechanism for melanogenesis inhibition, with qPCR and Western blotting revealing significant upregulation of Nrf2, HO-1, and NQO1. Molecular docking further supported oxypaeoniflorin's interactions with KEAP1 and Nrf2. These findings demonstrate oxypaeoniflorin's potential as a natural melanogenesis inhibitor and provide a scientific basis for developing POF-derived nutricosmetic ingredients.

分类号：

  • 相关文献

[1]Sulforaphane prevents LPS-induced inflammation by regulating the Nrf2-mediated autophagy pathway in goat mammary epithelial cells and a mouse model of mastitis. Shao, Dan,Shen, Wenxiang,Miao, Yuyang,Gao, Zhen,Pan, Menghao,Wei, Qiang,Yan, Zuoting,Zhao, Xiaoe,Ma, Baohua. 2023

[2]Neuroprotective effects of dendrobium endophytes metabolites in SH-SY5Y cells via the Nrf2/Keap1 pathway. Yan tian Liang,Jia meng Liu,Lu qi Qin,Cong Lu,Jing Sun,Qiong Wang,Yong Yang,Bei Fan,Feng zhong Wang. 2024

[3]Plant sterol ester of α-linolenic acid protects against ferroptosis in metabolic dysfunction-associated fatty liver disease via activating the Nrf2 signaling pathway. Hao Han,Liyuan Pei,Yajing Dong,Yanyang Han,Shuqi Ji,Xiaoman Wang,Mingming Zheng. 2025

[4]Wnt3a减轻黑素细胞iMC65氧化应激损伤. 郑妍,杨珂,李娅莎,董世访,周丽娜. 2017

[5]白藜芦醇对乳房链球菌感染引起乳腺细胞氧化应激 的影响及机制. 周艺琳,王正磊,许媛媛,韩先干,Vanhnaseng PHOUTHAPANE,苗晋锋. 2020

[6]Nrf2/Keap1-ARE通路的生物学功能及其信号调控. 冯志强,王腾飞,赵善江,郝海生,杜卫华,赵学明,邹惠影,朱化彬,庞云渭. 2022

[7]岩藻多糖对大鼠氧化应激的缓解作用研究. 徐元庆,张静,王哲奇,红雷. 2023

[8]Integrative analysis of transcriptomics and metabolomics to reveal the melanogenesis pathway of muscle and related meat characters in Wuliangshan black-boned chickens. Tengfei Dou,Shixiong Yan,Lixian Liu,Kun Wang,Zonghui Jian,Zhiqiang Xu,Jingying Zhao,Qiuting Wang,Shuai Sun,Mir Zulqarnain Talpur,Xiaohua Duan,Dahai Gu,Yang He,Yanli Du,Alsoufi Mohammed Abdulwahid,Qihua Li,Hua Rong,Weina Cao,Zhengchang Su,Guiping Zhao,Ranran Liu,Sumei Zhao,Ying Huang,Marinus F.W. Te Pas,Changrong Ge,Junjing Jia. 2022

[9]Anti-inflammatory effects of 4 '-demethylnobiletin, a major metabolite of nobiletin. Wu, Xian,Song, Mingyue,Rakariyatham, Kanyasiri,Zheng, Jinkai,Guo, Shanshan,Xiao, Hang,Zheng, Jinkai,Guo, Shanshan,Tang, Zhonghai,Zhou, Shuangde,Xiao, Hang.

[10]Nobiletin and its colonic metabolites suppress colitis-associated colon carcinogenesis by down-regulating iNOS, inducing antioxidative enzymes and arresting cell cycle progression. Wu, Xian,Song, Mingyue,Gao, Zili,Sun, Yue,Wang, Minqi,Li, Fang,Zheng, Jinkai,Xiao, Hang,Sun, Yue,Zheng, Jinkai.

[11]Allicin Improves Intestinal Epithelial Barrier Function and Prevents LPS-Induced Barrier Damages of Intestinal Epithelial Cell Monolayers. Jingxia Gao,Guanzhong Song,Haibo Shen,Yiming Wu,Chongqi Zhao,Zhuo Zhang,Qian Jiang,Xilong Li,Xiaokang Ma,Bie Tan,Yulong Yin. 2022

[12]AhR-mediated CYP1A1 and ROS overexpression are involved in hepatotoxicity of decabromodiphenyl ether (BDE-209). Jinwen Yuan,Xiaoming Sun,Siyan Che,Li Zhang,Zheng Ruan,Xiaomin Li,Junhua Yang. 2021

[13]Vitamin e supplementation enhances lipid oxidative stability via increasing vitamin e retention, rather than gene expression of mapk-nrf2 signaling pathway in muscles of broilers. Lei Xu,Jing Wang,Haijun Zhang,Shugeng Wu,Hongyuan Yue,Xiaoli Wan,Haiming Yang,Zhiyue Wang,Guanghai Qi. 2021

[14]Effects of isomerisation and oxidation on the immunomodulatory activity of chlorogenic acid in RAW264.7 macrophages. Guo, Chongting,Bi, Jinfeng,Li, Xuan,Lyu, Jian,Liu, Xuan,Liu, Jianing,Xu, Ye,Hu, Jiaxing. 2021

[15]Sulforaphane suppresses paraquat-induced oxidative damage in bovine in vitro-matured oocytes through Nrf2 transduction pathway. Zhiqiang Feng,Tengfei Wang,Yawen Sun,Siying Chen,Haisheng Hao,Weihua Du,Huiying Zou,Dawei Yu,Huabin Zhu,Yunwei Pang. 2023

[16]Structure-Based Identification of Kelch-like ECH-Associated Protein 1 as a Pharmacological Target of Electrophile-Containing Catechol-O-Methyltransferase Inhibitors. Wang, Ping,Li, Yang,Yang, Jinyi,Li, Ziwen,Ren, Xintong,Meng, Qingshi,Li, Pengfei,Qin, Luzhe,Li, Wei,Xie, Yuting,Hou, Nannan,Huang, Niu. 2024

[17]The modulation of metabolomics and antioxidant stress is involved in the effect of nitazoxanide against influenza A virus in vitro. Huang, Zhen,Zheng, Haihong,Wang, Yanping,Wang, Xiaoyang,Wang, Chunmei,Liu, Yingchun,Zhou, Wen,Wang, Zhaoxiong,Zhang, Keyu. 2023

[18]Long-term High-fat High-sucrose Diet Promotes Enlarged Islets and beta-Cell Damage by Oxidative Stress in Bama Minipigs. Zhao, Zhan-zhao,Xin, Lei-lei,Xia, Ji-han,Yang, Shu-lin,Li, Kui,Zhao, Zhan-zhao,Chen, Yao-xing. 2015

[19]Alterations in growth, oxidative damage, and metal uptake of five aromatic rice cultivars under lead toxicity. Ashraf, Umair,Tang, Xiangru,Ashraf, Umair,Hussain, Saddam,Anjum, Shakeel Ahmad,Abbas, Farhat,Tanveer, Mohsin,Noor, Mehmood Ali.

[20]Contribution of VMA5 to vacuolar function, stress response, ion homeostasis and autophagy in Candida albicans. Jia, Chang,Yu, Qilin,Xiao, Chenpeng,Dong, Yijie,Zhang, Meng,Zhang, Dan,Li, Mingchun,Dong, Yijie,Zhao, Qiang,Zhang, Biao.

作者其他论文 更多>>