数字农科院2.0

CsBZR4-CsRBOH5.1-BR module regulates opening/closure and expansion of cucumber cotyledons

文献类型： 外文期刊

作者： Lu Liu;Qinghua Di;Kunhao Xie;Xiaoqin Wang;Xianchang Yu;Yan Yan;Chaoxing He;Jun Wang;Mintao Sun;Yansu Li

作者机构：

关键词： Brassinosteroids;cotyledon;CsBZR4;CsRBOH5.1;Cucumber

期刊名称： International Journal of Biological Macromolecules

ISSN： 0141-8130

年卷期： 2025 年 334 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： The hydrogen peroxide (H2O2) plays diverse roles in physiological processes in plants. However, whether and how H2O2 regulates the opening and expansion of cotyledons in plants remains unknown. We demonstrated that CsRBOH5.1-encoded NADPH oxidase controls diurnal cotyledon opening/closure and expansion. Csrboh5.1 mutants exhibited defective cotyledon movements and reduced brassinosteroids (BR) levels, particularly 28-norbrassinolide and typhasterol. Both exogenous H2O2 and 24-epibrassinolide (EBR) treatments rescued these phenotypic defects. Molecular analyses revealed BR biosynthesis promotion by H2O2 and reciprocal regulation through CsBZR4 transcription factor. EBR upregulated CsBZR4 expression, which directly bound to and activated the CsRBOH5.1 promoter, as confirmed by yeast one-hybrid and dual-luciferase assays. This establishes a self-reinforcing regulatory module: CsRBOH5.1-derived H2O2 enhances BR synthesis, while BR-induced CsBZR4 transcriptionally activates CsRBOH5.1 expression. Transient overexpression assays in Csrboh5.1 mutants demonstrated that CsBZR4 did not directly regulate cotyledon opening. Our findings revealed the first plant RBOH-mediated H2O2 regulation of cotyledon development and elucidate a novel ‘CsBZR4-CsRBOH5.1-BR’ feedback mechanism coordinating redox signaling and phytohormone pathways. This module ensures precise control of cotyledon growth dynamics through reciprocal modulation of oxidative metabolism and brassinosteroids synthesis, providing new insights into the integration of redox biology and hormonal regulation in plant organogenesis.

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