数字农科院2.0

Transcriptome dynamics along with expression analysis of key genes involved in fiber development between Gossypium barbadense and Gossypium darwinii

文献类型： 外文期刊

作者： Allah Ditta;Xiaoyan Cai;Muhammad Shehzad;Sajid Fiaz;Yanchao Xu;Yuqing Hou;Zunaira Anwar;Aqsa Ijaz;Najla B.S. Al-Saud;Fang Liu;Kunbo Wang;Baohua Wang;Baohong Zhang;Ahmed M. Ramadan;Muhammad Kashif Riaz Khan;Zhongli Zhou

作者机构：

关键词： DPAs;Fiber development;Gene Functions;Single sequence repeats;Transcriptome profile

期刊名称： BMC Plant Biology

ISSN： 1471-2229

年卷期： 2025 年 25 卷 1 期

页码：

收录情况： SCIE（2025版）

摘要： The transcriptome profiling for underpinning the role of key genes controlling formation of fiber in cultivated Gossypium barbadense compared to wild allotetraploid cotton Gossypium darwinii which remained less investigated. Owing to excellent fiber quality of both Gossypium barbadense and Gossypium darwinii and information obtained via Simple Sequence Repeat (SSR) markers, two lines: Xh-18 and darwinii 5–7 were selected for transcriptome sequencing during developmental stages i.e., fiber initiation, elongation, and secondary cell wall (SCW) biosynthesis followed by 0 day after anthesis (DPA), 5DPA, 10DPA, 15DPA and 25DPA, respectively. Twelve libraries of RNA-seq were generated and sequenced individually generating approximately 818 million clean reads of Gossypium darwinii. However, for Gossypium barbadense more than 844 million clean reads were recorded. The Pearson Correlation Coefficient (PCC) analysis results indicated that gene expressions for both Gossypium barbadense and Gossypium darwinii indicated more than 90% of the commonalities at the same stage of fiber growth. However, genes found among Gossypium darwinii at 5, 6 and 7 DPA and XH-18 at 10 and 25 DPA were found disimilar. The expression quantity of RNA sequencing data, 31 genes were found common throughout all stages of DPAs in Gossypium darwinii 5–7 whereas, 377 genes were common in Gossypium barbadense XH-18 at 0, 5, 10, 15, 20, and 25 DPA stages of fiber development. Three genes XLOC_080616 (LTPG2_ARATH/ NLTL2_ARATH; GPI-anchored 2 non-specific lipid transfer protein, similar to At2 g13820), XLOC_065471 (LPAT2_ARATH/LPAT2_BRAOL 1-acyl-sn-glycerol-3-phosphate acyltransferase2), and XLOC_077416 (uncharacterized protein) shown up-regulated expression during 15 and 20 DPA for both Gossypium barbadense and Gossypium darwinii. It will also explore the possible role Gossypium barbadense DNA segment associated with development of fiber quality. Furthermore, this research will decipher the underlying process of fiber development and the possible role of genes for fiber formation in both Sea Island and wild cotton species.

分类号：

  • 相关文献

[1]2025-Transcriptome dynamics along with expression analysis of key genes involved in fiber development between Gossypium barbadense and Gossypium darwinii. . 2025

[2]Evaluation of the basic functions of six calcium-dependent protein kinases in Toxoplasma gondii using CRISPR-Cas9 system. Wang, Jin-Lei,Huang, Si-Yang,Li, Ting-Ting,Chen, Kai,Ning, Hong-Rui,Zhu, Xing-Quan,Huang, Si-Yang,Zhu, Xing-Quan.

[3]Effect of deleting four Toxoplasma gondii calcium-binding EGF domain-containing proteins on parasite replication and virulence. Wang, Xin-Cheng,Li, Ting-Ting,Elsheikha, Hany M. M.,Zheng, Xiao-Nan,Zhao, Dan-Yu,Wang, Jin-Lei,Wang, Meng,Zhu, Xing-Quan. 2022

[4]Generation of transcriptome profiling and gene functional analysis in Gossypium hirsutum upon Verticillium dahliae infection. Zhang, Huachong,Qi, Fangjun,Jian, Guiliang.

[5]Identifying potential RNAi targets in grain aphid (Sitobion avenae F.) based on transcriptome profiling of its alimentary canal after feeding on wheat plants. Zhang, Min,Wang, Hui,Wang, Dahai,Ma, Youzhi,Xia, Lanqin,Zhou, Yuwen,Gao, Qiang,Jones, Huw Dylan. 2013

[6]Genome-Wide Transcriptome Profiles of Rice Hybrids and Their Parents. Zhiguo E , Shanshan Huang , Yuping Zhang , Lei Ge , Lei Wang *. 2014

[7]Development and Regulation of the Extreme Biofilm Formation of Deinococcus radiodurans R1 under Extreme Environmental Conditions. Guo Q.,Zhan Y.,Zhang W.,Wang J.,Yan Y.,Wang W.,Lin M.. 2024

[8]Revitalizing Photoaging Skin through Eugenol in UVB-Exposed Hairless Mice: Mechanistic Insights from Integrated Multi-Omics. Tong, Tao,Geng, Ruixuan,Kang, Seong-Gook,Li, Xiaomin,Huang, Kunlun. 2024

[9]Fluctuation in levels of endogenous plant hormones in ovules of normal and mutant cotton during flowering and their relation to fiber development. Chen, JG,Du, XM,Zhao, HY,Zhou, X. 1996

[10]Specific expression of a beta-tubulin gene (GhTub1) in developing cotton fibers. Li, YL,Sun, J,Li, CH,Zhu, YQ,Xia, GX. 2003

[11]Cloning and Characterization of a CAP Gene Expressed in Gossypium arboreum Fuzzless Mutant. Sheng Wang,Guo-Hong Zhao,Yin-Hua Jia,Xiong-Ming Du.

[12]Gene-rich islands for fiber development in the cotton genome. Zhanyou Xu,Russell J. Kohel,Guoli Song,Jaemin Cho,Magdy Alabady,Jing Yu,Pamela Koo,Jun Chu,Shuxun Yu,Thea A. Wilkins,Yuxian Zhu,John Z. Yu. 2008

[13]Genome-wide characterization and expression analysis of MYB transcription factors in Gossypium hirsutum. Haron Salih,Wenfang Gong,Shoupu He,Gaofei Sun,Junling Sun,Xiongming Du. 2016

[14]Proteomic Analysis of Differences in Fiber Development between Wild and Cultivated Gossypium hirsutum L.. Yuan Qin,Hengling Wei,Huiru Sun,Pengbo Hao,Hantao Wang,Junji Su,Shuxun Yu.

[15]Genome-wide analysis and expression profiling of the phospholipase D gene family in Gossypium arboreum. Tang, Kai,Liu, Jinyuan,Dong, Chunjuan. 2016

[16]Genome-wide investigation and transcriptome analysis of the WRKY gene family in Gossypium. Mingquan Ding,Jiadong Chen,Yurong Jiang,Lifeng Lin,YueFen Cao,Minhua Wang,Yuting Zhang,Junkang Rong,Wuwei Ye.

[17]Genome-Wide Identification and Preliminary Functional Analysis of BAM (β-Amylase) Gene Family in Upland Cotton. Yanlong Yang,Fenglei Sun,Penglong Wang,Mayila Yusuyin,Wumaierjiang Kuerban,Chengxia Lai,Chunping Li,Jun Ma,Fei Xiao. 2023

[18]Comparative transcriptional and co-expression network analysis of two upland cotton accessions with extreme phenotypic differences reveals molecular mechanisms of fiber development. Jiasen He,Zhongyang Xu,Muhammad Tehseen Azhar,Zhen Zhang,Pengtao Li,Juwu Gong,Xiao Jiang,Senmiao Fan,Qun Ge,Youlu Yuan,Haihong Shang. 2023

[19]Ubiquitylome analysis reveals the involvement of ubiquitination in the bast fiber growth of ramie. Qiaoyun He,Zheng Zeng,Fu Li,Renyan Huang,Yanzhou Wang,Touming Liu. 2021

[20]RNA-Seq with a novel glabrous-ZM24fl reveals some key lncRNAs and the associated targets in fiber initiation of cotton. Xianyan Zou,Faiza Ali,Shuangxia Jin,Fuguang Li,Zhi Wang. 2022

作者其他论文 更多>>