数字农科院2.0

Genome-Wide and Expression Pattern Analysis of the HIT4 Gene Family Uncovers the Involvement of GHHIT4_4 in Response to Verticillium Wilt in Gossypium hirsutum

文献类型： 外文期刊

作者： Guoli Zhang;Yang Jiao;Zengqiang Zhao;Quanjia Chen;Zhijun Wang;Jincheng Zhu;Ning Lv;Guoqing Sun

作者机构：

关键词： expression pattern;G. hirsutum;HIT4;multiple synteny;phylogenetic analysis;transgenic tobacco;Verticillium wilt;VIGS;WGCNA

期刊名称： Genes

ISSN： 2073-4425

年卷期： 2024 年 15 卷 3 期

页码：

收录情况： SCIE（2024版）

摘要： Chromatin remodelers are essential for regulating plant growth, development, and responses to environmental stresses. HIT4 (HEAT-INTOLERANT 4) is a novel stress-induced chromatin remodeling factor that has been less studied in abiotic stress and stress resistance, particularly in cotton. In this study, we conducted a comprehensive analysis of the members of the HIT4 gene family in Gossypium hirsutum using bioinformatics methods, including phylogenetic relationships, gene organization, transcription profiles, phylogenetic connections, selection pressure, and stress response. A total of 18 HIT4 genes were identified in four cotton species, with six HIT4 gene members in upland cotton. Based on the evolutionary relationships shown in the phylogenetic tree, the 18 HIT4 protein sequences were classified into four distinct subgroups. Furthermore, we conducted chromosome mapping to determine the genomic locations of these genes and visually represented the structural characteristics of HIT4 in G. hirsutum. In addition, we predicted the regulatory elements in HIT4 in G. hirsutum and conducted an analysis of repetitive sequences and gene collinearity among HIT4 in four cotton species. Moreover, we calculated the Ka/Ks ratio for homologous genes to assess the selection pressure acting on HIT4. Using RNA-seq, we explored the expression patterns of HIT4 genes in G. hirsutum and Gossypium barbadense. Through weighted gene co-expression network analysis (WGCNA), we found that GHHIT4_4 belonged to the MEblue module, which was mainly enriched in pathways such as DNA replication, phagosome, pentose and glucuronate interconversions, steroid biosynthesis, and starch and sucrose metabolism. This module may regulate the mechanism of upland cotton resistance to Verticillium wilt through DNA replication, phagosome, and various metabolic pathways. In addition, we performed heterologous overexpression of GH_D11G0591 (GHHIT4_4) in tobacco, and the results showed a significant reduction in disease index compared to the wild type, with higher expression levels of disease resistance genes in the transgenic tobacco. After conducting a VIGS (virus-induced gene silencing) experiment in cotton, the results indicated that silencing GHHIT4_4 had a significant impact, the resistance to Verticillium wilt weakened, and the internode length of the plants significantly decreased by 30.7% while the number of true leaves increased by 41.5%. qRT-PCR analysis indicated that GHHIT4_4 mainly enhanced cotton resistance to Verticillium wilt by indirectly regulating the PAL, 4CL, and CHI genes. The subcellular localization results revealed that GHHIT4_4 was predominantly distributed in the mitochondria and nucleus. This study offers preliminary evidence for the involvement of the GHHIT4_4 in cotton resistance to Verticillium wilt and lays the foundation for further research on the disease resistance mechanism of this gene in cotton.

分类号：

  • 相关文献

[1]Molecular structures and functional exploration of NDA family genes respond tolerant to alkaline stress in Gossypium hirsutum L.. Yapeng Fan,Yuexin Zhang,Cun Rui,Hong Zhang,Nan Xu,Jing Wang,Mingge Han,Xuke Lu,Xiugui Chen,Delong Wang,Shuai Wang,Lixue Guo,Lanjie Zhao,Hui Huang,Junjuan Wang,Liangqing Sun,Chao Chen,Wuwei Ye. 2022

[2]Genomic identification of cotton SAC genes branded ovule and stress-related key genes in Gossypium hirsutum. Ma Shuya,Liu Le,Shi Huiyun,Gu Yu,Li Yujun,Ghulam Qanmber. 2023

[3]Functional characterization of TBL genes revealed the role of GhTBL7 and GhTBL58 in cotton fiber elongation. Nosheen Kabir,Xuwen Wang,Lili Lu,Ghulam Qanmber,Le Liu,Aijun Si,Lian Zhang,Wei Cao,Zuoren Yang,Yu Yu,Zhao Liu. 2023

[4]Molecular cloning and characterization of enhanced disease susceptibility 1 (EDS1) from Gossypium barbadense. Su, Xiaofeng,Qi, Xiliang,Cheng, Hongmei.

[5]Characterization, Expression, and Functional Analysis of a Novel NAC Gene Associated with Resistance to Verticillium Wilt and Abiotic Stress in Cotton. Wang, Weina,Geng, Shuaipeng,Sun, Quan,Long, Lu,Cai, Chaowei,Liu, Xin,Wang, Guanghao,Miao, Chen,Zhang, Xiao,Cai, Yingfan,Wang, Weina,Yang, Can,Yuan, Youlu,Du, Xiongming,Chu, Zongyan. 2016

[6]Molecular characterisation and functional analysis of a cytochrome P450 gene in cotton. Long, Lu,Sun, Quan,Wang, Weina,Gao, Wei,Cai, Chaowei,Mo, Jianchuan,Cheng, Jieru,Zhang, Xiangrui,Liu, Yujia,Miao, Chen,Zhang, Xiao,Cai, Yingfan,Sun, Quan,Wang, Weina,Du, Xiongming,Shi, Yuzhen,Yuan, Youlu,Chu, Zongyan.

[7]Terpene synthases GhTPS6 and GhTPS47 participate in resistance to Verticillium dahliae in upland cotton. Wei Liu,Zhiqiang Zhang,Yuchen Wu,Yuzhi Zhang,Xiaona Li,Jianing Li,Wei Zhu,Zongbin Ma,Wei Li. 2024

[8]Dynamic characteristics and functional analysis provide new insights into the role of GauERF105 for resistance against Verticillium dahliae in cotton. Yanqing Wang,Muhammad Jawad Umer,Xiaoyan Cai,Mengying Yang,Yuqing Hou,Yanchao Xu,Raufa Batool,Teame Gereziher Mehari,Jie Zheng,Yuhong Wang,Heng Wang,Zhikun Li,Zhongli Zhou,Fang Liu. 2023

[9]Transcriptome Analysis and VIGS Identification of Key Genes Regulating Citric Acid Metabolism in Citrus. Chen, Tianxin,Niu, Juan,Sun, Zhimin,Chen, Jing,Wang, Yue,Chen, Jianhua,Luan, Mingbao. 2023

[10]Integrated Transcriptomic and Metabolomic Analysis Reveals GHABF3-Mediated ABA Signaling Governs Enhanced Verticillium Wilt Resistance in Cotton. Pengtao Li,Rui Yang,Zhihao Sun,Qun Ge,Xianghui Xiao,Shuhan Yang,Yanfang Li,Qiankun Liu,Xiaoyan Liu,Dongxia Wang,Juwu Gong,Quanwei Lu,Yuzhen Shi,Renhai Peng,Haihong Shang,Youlu Yuan,Yu Chen,Wankui Gong. 2025

[11]Identification of core candidate genes responding to Verticillium wilt (Verticillium dahliae) in cotton via integrated methods. Zengqiang Zhao,Guoli Zhang,Hang Yu,Guoqing Sun,Jianbo Zhu. 2025

[12]Genome-wide characterization and VIGS-mediated silencing reveal that GHBSL7 enhances drought tolerance in cotton. Li, Yanfang,Xing, Baoguang,Zhang, Shaoliang,Chen, Guodong,Zhao, Yongqing,Liu, Qiankun,Hao, Liuan,Gong, Wankui,Li, Pengtao,Peng, Renhai,Wan, Sumei. 2025

[13]Genome-wide characterization of trichome birefringence-like genes provides insights into fiber yield improvement. Ziyin Li,Yuzhen Shi,Xianghui Xiao,Jikun Song,Pengtao Li,Juwu Gong,Haibo Zhang,Wankui Gong,Aiying Liu,Renhai Peng,Haihong Shang,Qun Ge,Junwen Li,Jingtao Pan,Quanjia Chen,Quanwei Lu,Youlu Yuan. 2023

[14]Molecular identification and expression patterns of carboxylesterase genes based on transcriptome analysis of the common cutworm, Spodoptera litura (Lepidoptera: Noctuidae). Zhang, Ya-Nan,Li, Jin-Bu,Fang, Li-Ping,Deng, Dao-Gui,Zhu, Xiu-Yun,He, Peng,Sun, Liang,Li, Zhao-Qun,Ye, Zhan-Feng. 2016

[15]Genome-wide identification of auxin response factor (ARF) genes and its tissue-specific prominent expression in Gossypium raimondii. Runrun Sun,Kunbo Wang,Tenglong Guo,Don C. Jones,Juliana Cobb,Baohong Zhang,Qinglian Wang.

[16]Identification of a CONSTANS homologous gene with distinct diurnal expression patterns in varied photoperiods in ramie (Boehmeria nivea L. Gaud). Liu, Touming,Zhu, Siyuan,Tang, Qingming,Tang, Shouwei.

[17]Identification of 32 full-length NAC transcription factors in ramie (Boehmeria nivea L. Gaud) and characterization of the expression pattern of these genes. Liu, Touming,Zhu, Siyuan,Tang, Qingming,Tang, Shouwei.

[18]Revealing the Roles of the JAZ Family in Defense Signaling and the Agarwood Formation Process in Aquilaria sinensis. Yimian Ma,Jiadong Ran,Guoqiong Li,Mengchen Wang,Chengmin Yang,Xin Wen,Xin Geng,Liping Zhang,Yuan Li,Zheng Zhang. 2023

[19]Identification, evolutionary analysis and functional diversification of RAV gene family in cotton (G. hirsutum L.). Nosheen Kabir,Hai Lin,Xianhui Kong,Le Liu,Ghulam Qanmber,Yu Xuan Wang,Lian Zhang,Zhuojing Sun,Zuoren Yang,Yu Yu,Na Zhao. 2022

[20]Genome-Wide Identification and Evolutionary Analysis of Functional BBM-like Genes in Plant Species. Zhengyuan Hong,Linghong Zhu,Chaolei Liu,Kejian Wang,Yuchun Rao,Hongwei Lu. 2025

作者其他论文 更多>>