数字农科院2.0

Identification and characterization of aldo-keto reductase responsible for patulin degradation in Saccharomyces cerevisiae

文献类型： 外文期刊

作者： Chao Yang;Lingxuan Huang;Chen Hu;Jieqiong Yao;Ting Zhou;Xiu Zhen Li;Stephen Y.K. Seah;Bangzhu Peng

作者机构：

关键词： （0-1-5）Aldo-keto reductase;Biodegradation;Molecular docking;Patulin

期刊名称： Food Chemistry

ISSN： 0308-8146

年卷期： 2025 年 478 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Patulin (PAT) is a hazardous mycotoxin that contaminates fruits and their products, causing significant economic losses. An aldo-keto reductase from Saccharomyces cerevisiae (ScAKR) was expressed in Escherichia coli in this study. The purified ScAKR converted PAT to E-ascladiol with NADPH as a cofactor. The ScAKR exhibited a strong degradation activity on PAT and the optimal degradation conditions were pH 7 and 37 °C. Molecular docking and site-specific mutagenesis indicated that the amino acids in ScAKR interacting with PAT aldehyde affected the degradation effect, and the mutation of Trp298 showed the most significant effect on the degradation rate. Furthermore, ScAKR also showed a strong degradation effect on 3-keto-deoxynivalenol, a metabolite of another mycotoxin, deoxynivalenol (DON). The findings offer new insights on the detoxification mechanism of PAT by S. cerevisiae and for the development and application of bioenzymes with broad-spectrum mycotoxin degradation properties.

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