数字农科院2.0

The escape of CRISPR-mediated gene editing in Zymomonas mobilis

文献类型： 外文期刊

作者： Mao Chen;Yuhuan Huang;Yudi Zheng;Bo Wu;Mingxiong He

作者机构：

关键词： Zymomonas mobilis;CRISPR;gene escape;knockout;literature review;plasmid curing

期刊名称： FEMS microbiology letters

ISSN： 1574-6968

年卷期： 2023 年 370 卷

页码：

收录情况： SCIE（2023版）

摘要： Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) systems have been widely applied for gene or genome editing. Adequate checking is important to screen mutants after CRISPR-mediated editing events. Here, we report gene escape cases after the knockout by Type I-F native CRISPR system in Zymomonas mobilis. Through amplifying both the gene of interest and its flanking homologous arms, followed by curing the editing plasmid, we found different destinies for gene-editing events. Some genes were readily knocked out and followed by the easy plasmid curing. In some other cases, however, the editing plasmid was difficult to remove from the cell, or the deleted genes were transferred into the editing plasmid. For example, the targeted region of fur can be integrated into the editing plasmid after the knockout, resulting in a spurious editing event. We supposed that the transfer of the gene may be attributed to bacterial insertion sequences. Searching for literatures on the gene knockout using CRISPR in bacteria reveals that the escape event is likely underestimated due to inadequate validation in other microbes. Hence, several strategies are proposed to enhance gene knockout and plasmid curing.

分类号：

  • 相关文献

[1]Curing a large endogenous plasmid by single substitution of a partitioning gene. Song, Ningning,Li, Zhaoli,Li, Zhaoli,Hernalsteens, Jean-Pierre,Song, Ningning,Xu, Jie.

[2]A Review on the Adoption of AI, BC, and IoT in Sustainability Research. Susie Ruqun Wu,Gabriela Shirkey,Ilke Celik,Changliang Shao,Jiquan Chen. 2022

[3]Rural revival: Navigating environmental engineering and technology. Siyuan Yang,Xiangqun Zheng,Jiaqi Hou,Bing Geng,Liangguo Luo,Changxiong Zhu,Liyuan Liu,Jie Zhu. 2024

[4]TALEN construction for porcine I kappa B alpha gene and the detection of knockout activity. Xu, Kui,Ruan, Jinxue,Li, Hegang,Wu, Tianwen,Liang, Bo,Mu, Yulian,Li, Hegang.

[5]Adipocyte miR-200b/a/429 ablation in mice leads to high-fat-diet-induced obesity. Tao, Cong,Ren, Hongyan,Xu, Pan,Cheng, Jia,Huang, Sujuan,Zhou, Rong,Mu, Yulian,Yang, Shulin,Wang, Yanfang,Li, Kui,Tao, Cong,Qi, Desheng,Ren, Hongyan. 2016

[6]Fluorescent transgenic silkworm. Zhang, F,Zhao, Y,Chen, X,Xu, AY,Huang, JT,Lu, CD. 1999

[7]运用CRISPR/Cas系统对植物基因组进行定点编辑. 宋时奎,王影,于洋,耿立召,张春翔,李相敢,韩天富. 2014

[8]基因编辑技术及其在作物育种中的应用与安全管理. ,,龙艳,,裴新梧. 2018

[9]CRISPR技术在病毒学研究中的应用. 高帅,李芳,李赞,王涛,赵东明,步志高. 2020

[10]基于专利文献的全球CRISPR技术研发进展分析与展望. 王友华1 邹婉侬1 张熠2 樊君丽3 孙国庆1. 2018

[11]利用CRISPR技术鉴定拟南芥miR171a的功能. 黄格格,张静文,张天豹,柯智,翟楠鑫,龙艳,袁潜华,裴新梧. 2019

[12]基于CRISPR的快速灵敏便捷分子检测. 孙雯君,黄行许,王鑫杰. 2023

[13]基于CRISPR系统的基因编辑技术的研究进展. 郑基坛(综述),闫娜娜,左二伟(审校). 2021

[14]水稻CRISPR/Cas12a系统的优化及其介导的腺嘌呤碱基编辑器的建立. 王敬文,严芳,柳浪,周雪平,王道文,周焕斌. 2021

[15]CRISPR相关技术在肉牛领域的研究进展. 吴天弋,党靖宇,王添祯,张路培,高雪,李俊雅,徐凌洋. 2023

[16]基于CRISPR/CasX介导的水稻基因编辑技术的建立. 李雪琪,张素杰,于曼,黄金光,周焕斌. 2023

[17]基于CRISPR/LanCas9的水稻基因编辑系统的开发和优化. 李欣格,王美霞,王晨阳,马桂根,周常勇,王亚南,周焕斌. 2024

[18]植物线粒体基因组编辑研究进展. 张硕,阚俊虎,周家伟,武志强. 2024

[19]家禽基因编辑相关技术研究进展及应用. 冉明霞,郑基坛,刘兴廷,谢龙,左二伟,陆阳清. 2024

[20]RUBY辅助的水稻高效CRISPR基因编辑. 邓美壁,严浪,詹志田,朱敏,和玉兵. 2025

作者其他论文 更多>>