数字农科院2.0

Heterologous Expression Of Ahl Lactonase Aiik By Lactobacillus Casei Mcj Delta 1 With Great Quorum Quenching Ability Against Aeromonas Hydrophila Ah-1 And Ah-4

文献类型： 外文期刊

作者： Dong, WW; Cai, YY; Xu, ZL; Fu, B; Chen, QT; Cui, YX; Ruan, ZY; Liang, YX; Peng, N; Zhao, SM

作者机构：

关键词： Quorum Sensing; Quorum Quenching; Ahl Lactonase Aiik; Lactobacillus Casei Mcj Delta 1; Aeromonas Hydrophila

期刊名称： MICROBIAL CELL FACTORIES

ISSN：

年卷期： 2020 年 19 卷 1 期

页码：

收录情况： JCR（2021版）

摘要： Background: Nowadays, microbial infections have caused increasing economic losses in aquaculture industry and deteriorated worldwide environments. Many of these infections are caused by opportunistic pathogens through cell-density mediated quorum sensing (QS). The disruption of QS, known as quorum quenching (QQ), is an effective and promising way to prevent and control pathogens, driving it be the potential bio-control agents. In our previous studies, AHL lactonase AiiK was identified with many characteristics, and constitutive expression vector pELX1 was constructed to express heterologous proteins in Lactobacillus casei MCJ Delta 1 (L. casei MCJ Delta 1). In this study, recombinant strain pELCW-aiiK/L. casei MCJ Delta 1 (LcAiiK) and wild-typeAeromonas hydrophila (A. hydrophila) were co-cultured to test the QQ ability of LcAiiK againstA. hydrophila. Results: A cell wall-associated expression vector pELCW forL. casei MCJ Delta 1 was constructed. Localization assays revealed that the expressed AiiK was anchored at the surface layer of LcAiiK via vector pELCW-aiiK. LcAiiK (OD600 = 0.5) degraded 24.13 mu M of C-6-HSL at 2 h, 40.99 mu M of C-6-HSL at 12 h, and 46.63 mu M of C-6-HSL at 24 h. Over 50% LcAiiK cells maintained the pELCW-aiiKplasmid after 15 generations of cultivation without erythromycin. Furthermore, LcAiiK inhibited the swimming motility, extracellular proteolytic activity, haemolytic activity and biofilm formation ofA. hydrophila AH-1 and AH-4. Conclusion: The AHL lactonase AiiK is firstly and constitutively expressed at the surface layer ofL. casei MCJ Delta 1. LcAiiK displayed considerable AHL lactonase activity and great QQ abilities againstA. hydrophila AH-1 and AH-4 by attenuating their QS processes instead of killing them. Therefore, the LcAiiK can be exploited as an anti-pathogenic drug or a bio-control agent to control the AHL-mediated QS of pathogenic bacteria.

分类号：

  • 相关文献

[1]Improving The Production Of Ahl Lactonase Aiio-Aio6 Fromochrobactrumsp. M231 In Intracellular Protease-Deficientbacillus Subtilis. Xia, R, Yang, YL, Pan, XL, Gao, CC, Yao, YY, Liu, XW, Teame, T, Zhang, FL, Hu, J, Ran, C, Zhang, Z, Liu-Clarke, JH, Zhou, ZG. 2020

[2]The luxS gene functions in the pathogenesis of avian pathogenic Escherichia coli. Han, Xiangan,Bai, Hao,Liu, Lei,Dong, Hongliang,Liu, Rui,Song, Jun,Ding, Chan,Liu, Haiwen,Yu, Shengqing,Qi, Kezong. 2013

[3]The effect of quorum sensing on anaerobic granular sludge in different pH conditions. Feng, Huajun,Li, Na,Zhou, Yuyang,Wang, Meizhen,Zhang, Xueqin,Shen, Dongsheng,Huang, Wenkun.

[4]Rapid method of luxS and pfs gene inactivation in enterotoxigenic Escherichia coli and the effect on biofilm formation. Wang, Xiaoxu,Li, Shiyu,Lu, Xiaoran,Li, Zheng,Bu, Zhaoyang,Lang, Xulong,Wang, Xinlong,Wang, Xiaoxu,Hu, Pan,Chen, Haiyan.

[5]A sustainable method for effective regulation of anaerobic granular sludge: Artificially increasing the concentration of signal molecules by cultivating a secreting strain. Feng, Huajun,Shen, Dongsheng,Wang, Meizhen,Huang, Wenkun.

[6]Quorum-sensing contributes to virulence, twitching motility, seed attachment and biofilm formation in the wild type strain Aac-5 of Acidovorax citrulli. Guan, Wei,Yang, Yuwen,Yan, Wanrong,Sun, Baixin,Zhao, Tingchang,Wang, Tielin,Huang, Qi.

[7]Burkholderia Cenocepacia Integrates Cis-2-Dodecenoic Acid A.nd Cyclic Dimeric Guanosine M onophosphate Signals To Control Virulence. Yang, CX, Cui, CY, Ye, QM, Kan, JH, Fu, SN, Song, SH, Huang, YT, He, F, Zhang, LH, Jia, YT, Gao, YG, Harwood, CS, Deng, YY. 2017

[8]Phytopathogenic bacteria utilize host glucose as a signal to stimulate virulence through LuxR homologues. Zhang, Siyuan,Kan, Jinhong,Liu, Xin,Wu, Yao,Zhang, Mingyang,Ou, Jinqing,Wang, Juan,An, Lin,Li, Defeng,Wang, Li,Wang, Xiu-Jie,Fang, Rongxiang,Jia, Yantao. 2023

[9]Novel Antibiofilm Inhibitor Ginkgetin as an Antibacterial Synergist against Escherichia coli. Bai, Yubin,Wang, Weiwei,Shi, Mengyan,Wei, Xiaojuan,Zhou, Xuzheng,Li, Bing,Zhang, Jiyu. 2022

[10]A Novel and Efficient Platform for Discovering Noncanonical Quorum-Quenching Proteins. Liao J.,Li Z.,Xiong D.,Shen D.,Wang L.,Shao X.,Li T.,Qian G.. 2023

[11]Exploiting enzymes as a powerful tool to modulate the gut microbiota. Xiaoyun Su,Bin Yao. 2022

[12]Novel quorum sensing inhibitor Echinatin as an antibacterial synergist against Escherichia coli. Yu Bin Bai,Meng Yan Shi,Wei Wei Wang,Ling Yu Wu,Yu Ting Bai,Bing Li,Xu Zheng Zhou,Ji Yu Zhang. 2022

[13]Virtual Screening and In Vitro Experimental Verification of LuxS Inhibitors for Escherichia coli O157:H7. Yu Bin Bai,Xiao Rong Yang,Bing Li,Xu Zheng Zhou,Wei Wei Wang,Fu Sheng Cheng,Ji Yu Zhang. 2023

[14]Bacterial quorum sensing quenching activity of Lysobacter leucyl aminopeptidase acts by interacting with autoinducer synthase. Jinxing Liao,Danyu Shen,Long Lin,Hongjun Chen,Yajie Jin,Shan Ho Chou,Xiao Quan Yu,Tao Li,Guoliang Qian. 2021

[15]Complete genome sequence provides information on quorum sensing related spoilage and virulence of Aeromonas salmonicida GMT3 isolated from spoiled sturgeon. Lei Liu,Jun Li,Mingxia Tu,Liang Gao,Ying Zhang,Yu Rao,Lei Rao,Meng Gui. 2024

[16]Biofilm formation, occurrence, microbial communication, impact and characterization methods in natural and anthropic systems: a review. Elumalai, Punniyakotti,Gao, Xuke,Cui, Jinjie,Kumar, Arunagiri Santhosh,Dhandapani, Perumal,Parthipan, Punniyakotti,Karthikeyan, Obulisamy Parthiba,Theerthagiri, Jayaraman,Kheawhom, Soorathep,Choi, Myong Yong. 2024

[17]Targeting AI-2 quorum sensing: harnessing natural products against Streptococcus suis biofilm infection. Shuji Gao,Shuo Yuan,Yingying Quan,Wenjie Jin,Yamin Shen,Rishun Li,Baobao Liu,Yuxin Wang,Li Yi,Shaohui Wang,Xiaogai Hou,Yang Wang. 2025

[18]Decoding fungal communication networks: molecular signaling, genetic regulation, and ecological implications. Shumila Ishfaq,Hadiqa Anum,Tayyaba Shaheen,Sana Zulfiqar,Anila Ishfaq,Arslan Anjum,Umera Ramzan,Amna Rafiq,Mehboob-ur-Rahman,Wei Guo. 2025

[19]Lactic acid bacteria and yeast co-culture system: Mechanism of quorum-sensing regulation on the high yield of exopolysaccharides in Weissella confusa. Wenna Yu,Ziwen Wang,Huiran Zhao,Yang Qiu,Zehai Chen,Jingping Ge,Renpeng Du. 2025

[20]Resveratrol blocks the spoilage and virulence phenotypes of Aeromonas salmonicida isolated from sturgeon by inhibiting quorum sensing. Mingxia Tu,Yu Rao,Lei Liu,Chunming Tan,Liang Gao,Jun Li,Xia Gao,Meng Gui. 2026

作者其他论文 更多>>