数字农科院2.0

Niemann-Pick type C2 protein PpseNPC2–1 binding volatile nerolidol mediates prey localization of the pond wolf spider Pardosa pseudoannulata

文献类型： 外文期刊

作者： Fuqiang Rao;Chunli Xiu;Dehai Zhao;Shuang Shan;Yu Gao;Xiaoming Cai;Zhaoqun Li;Zewen Liu;Yongjun Zhang

作者机构：

关键词： HIPVs;Nerolidol;Niemann-pick type C2;Pardosa pseudoannulata;Plant indirect defense;RNA interference

期刊名称： International Journal of Biological Macromolecules

ISSN： 0141-8130

年卷期： 2025 年 322 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： As a key natural enemy of common agricultural pests, the pond wolf spider Pardosa pseudoannulata preys on planthoppers, leafhoppers and other pests. Chemoreception is essential for P. pseudoannulata in locating prey and searching for mates. Although our previous work identified Niemann-Pick type C2 (NPC2) proteins as olfactory-related in P. pseudoannulata, the molecular mechanisms underlying prey detection remain unclear. Here, we demonstrated that the P. pseudoannulata spiders showed a significant preference for rice plants infested with Nilaparvata lugens (brown planthoppers). Nerolidol, a major component of herbivore-induced plant volatiles (HIPVs), elicited robust electrophysiological and behavioral responses in the spiders. Real-time quantitative PCR (qPCR) and in situ hybridization revealed that PpseNPC2–1 was highly expressed in the pedipalps—the primary chemosensory appendages. Fluorescence binding assays showed that recombinant PpseNPC2–1 protein bound strongly to nerolidol with a dissociation constant (Ki) below 10 μM. Molecular docking further validated the strong binding interaction between PpseNPC2–1 and nerolidol. RNA interference (RNAi)-mediated knockdown of PpseNPC2–1, which achieved a 90.2 ± 2.4 % reduction in transcript levels, abolished the spiders' behavioral preference for nerolidol. These findings establish that PpseNPC2–1 plays a crucial role in perceiving HIPVs in P. pseudoannulata, which aids spiders in detecting their prey and promotes the plant's indirect defense.

分类号：

  • 相关文献

[1]Odorant receptor CaspOR39 underpinned cineole-mediated oviposition repellency in the rape stem weevil Ceutorhynchus asper. Rao, Fuqiang,Yang, Jie,Peng, Danhao,Li, Xinghao,Li, Zhaoqun,Liu, Deguang. 2025

[2]Electrophysiological Responses and Reproductive Behavior of Fall Webworm Moths (Hyphantria cunea Drury) are Influenced by Volatile Compounds from Its Mulberry Host (Morus alba L.). Tang, Rui,Zhang, Zhong-Ning,Tang, Rui,Zhang, Feng,Tang, Rui,Zhang, Feng. 2016

[3]Specific loops D, E and F of nicotinic acetylcholine receptor beta 1 subunit may confer imidacloprid selectivity between Myzus persicae and its predatory enemy Pardosa pseudoannulata. Song, Feng,Yao, Xiangmei,Liu, Zewen,You, Zhiqi,Cheng, Jiagao,Lin, Kejian. 2009

[4]Transcriptomic Response Of Wolf Spider, P.ardosa Pseudoannulata, To Transgenic R ice Expressing Bacillus Thuringiensis Cry1Ab Protein. Wang, J, Peng, YD, Xiao, KF, Wei, BY, Hu, JL, Wang, Z, Song, QS, Zhou, XG. 2017

[5]Niemann-Pick Proteins Type C2 Are I.dentified As Olfactory Related G enes Of Pardosa Pseudoannulata By Transcriptome And Expression Profile Analysis. Zhang, Song,Xiao, Yong,Xiu, Chunli,Zhang, Yongjun,Xiu, Chunli,Xiao, Yong,Liu, Zewen,Bao, Haibo. 2019

[6]Multiple acetylcholinesterases in Pardosa pseudoannulata brain worked collaboratively to provide protection from organophosphorus insecticides. Xumin Lin,Yixi Zhang,Baojun Yang,Lingchun Zhang,Yunru Chen,Zewen Liu. 2022

[7]Herbivore-induced volatiles influence moth preference by increasing the β-Ocimene emission of neighbouring tea plants. Tingting Jing,Xiaona Qian,Wenkai Du,Ting Gao,Dongfeng Li,Danyang Guo,Fan He,Guomeng Yu,Shupeng Li,Wilfried Schwab,Xiaochun Wan,Xiaoling Sun,Chuankui Song. 2021

[8]Integrative analysis identifies the safety of transgenic Cry1Ab rice to non-target spider Pardosa pseudoannulata. Bo Lv,Ying Zhou,Yuan de Peng,Zhi Wang,Qi sheng Song. 2022

[9]Integrated transcriptomics and proteomics provide new insights into the cadmium-induced ovarian toxicity on Pardosa pseudoannulata. Bo Lv,Yong Peng,Yuan de Peng,Zhi Wang,Qi sheng Song. 2022

[10]Long-Term Cadmium Exposure Affects Cell Adhesion And Expression Of Cadherin In The Male Genital Organ Of Pardosa Pseudoannulata (Bosenberg & Strand, 1906). Lv, B, Wang, J, Peng, YD, Wang, Z, Song, QS. 2020

[11]Two jasmonic acid carboxyl methyltransferases in Gossypium hirsutum involved in MeJA biosynthesis may contribute to plant defense. Dong Teng,Weixia Jing,Beibei Lv,Xinzheng Huang,Danyang Zhao,Junfeng Kou,Xiaohe Liu,Khalid Hussain Dhiloo,Yongjun Zhang. 2023

[12]Integrated Volatile Metabolomics and Transcriptomics Analyses Reveal the Influence of Infection TuMV to Volatile Organic Compounds in Brassica rapa. Xinxin Lu,Lei Zhang,Wenyue Huang,Shujiang Zhang,Shifan Zhang,Fei Li,Hui Zhang,Rifei Sun,Jianjun Zhao,Guoliang Li. 2022

[13]Recruitment of Hippodamia variegata by active volatiles from Glycyrrhiza uralensis and Alhagi sparsifolia plants infested with Aphis atrata. Jiang, Yan,Xiu, Chunli,Pan, Hongsheng,Liu, Xiaoning. 2023

[14]Spodoptera frugiperda Salivary Glucose Oxidase Reduces the Release of Green Leaf Volatiles and Increases Terpene Emission from Maize. Bin Gao,Bin Li,Jinxi Yuan,Zhan Shi,Xialin Zheng,Guirong Wang. 2024

[15]Characterization of core maize volatiles induced by Spodoptera frugiperda that alter the mating-mediated approach-avoidance behaviors of Mythimna separata. Yang, Denghai,Fan, Hengzhe,Hu, Ruyi,Huang, Yong,Sheng, Chengwang,Cao, Haiqun,Yang, Bin,Jiang, Xingchuan. 2025

[16]Long-term cadmium stress influenced the immune response of Pardosa pseudoannulata by modulating its gut microbiota and hemolymph metabolome. Jinjin Li,Yuan de Peng,Rong Zhou,Jie Peng,Qingling She,Xinyi Yang,Juan Wang,Zhi Wang,Qisheng Song. 2024

[17]Knockdown of a putative alanine aminotransferase gene affects amino acid content and flight capacity in the Colorado potato beetle Leptinotarsa decemlineata. Wan, Pin-Jun,Fu, Kai-Yun,Lu, Feng-Gong,Li, Guo-Qing,Guo, Wen-Chao,Wan, Pin-Jun.

[18]Effective inhibition of Japanese encephalitis virus replication by small interfering RNAs targeting the NS5 gene. Qi, Wen-Bao,Hua, Rong-Hong,Yan, Li-Ping,Tong, Guang-Zhi,Wu, Dong-Lai,Qi, Wen-Bao,Tong, Guang-Zhi,Zhang, Gui-Hong,Ren, Tao,Liao, Ming. 2008

[19]The effect of silencing arginine kinase by RNAi on the larval development of Helicoverpa armigera. Su, X. -F.,Lu, G. -Q.,Cheng, H. -M.,Liu, C. -X.,Liang, G. -M..

[20]Silencing in Apolygus lucorum of the olfactory coreceptor Orco gene by RNA interference induces EAG response declining to two putative semiochemicals. Zhu, Xiao-Qiang,Gu, Shao-Hua,Cui, Huan-huan,Guo, Yu-Yuan,Zhang, Yong-Jun,Zhou, Jing-Jiang. 2014

作者其他论文 更多>>