数字农科院2.0

The host transcriptome change involved in the inhibitory effect of exogenous interferon-γ on Getah virus replication

文献类型： 外文期刊

作者： Jialei Li;Xintao Gao;Xingjian Liu;Tong Wu;Haozhi Song;Weisong Gao;Hong Jia;Yinü Li;Zhifang Zhang

作者机构：

关键词： Getah virus;IFN-γ;immune escape;inhibition of replication;transcriptome change

期刊名称： Frontiers in Microbiology

ISSN： 1664-302X

年卷期： 2023 年 14 卷

页码：

收录情况： SCIE（2023版）

摘要： Introduction: Getah virus (GETV) has become a growing potential threat to the global livestock industry and public health. However, little is known about the viral pathogenesis and immune escape mechanisms, leading to ineffective control measures. Methods: In this study, the antiviral activity of exogenous interferons (IFNs) was assessed by using western blotting (WB), real-time quantitative PCR (RT-qPCR) and indirect immunofluorescence assay (IFA). The comparative transcriptomics among mock- and GETV-infected (MOI = 0.1) ST cells with or without IFN-γ was performed by RNA-seq, and then the transcriptome profiling of GETV-infected ST cells and key pathways and putative factors involved in inhibitory effect of IFN-γ on GETV replication were analyzed by bioinformatics methods and RT-qPCR. Results: The results showed that treatment with IFN-γ could suppress GETV replication, and the inhibitory effect lasted for at least 48 h, while the exogenous IFN-α/ω and IFN-λ3 treatments failed to inhibit the viral infection and early replication in vitro. Furthermore, the blueprint of virus-host interaction was plotted by RNA-seq and RT-qPCR, showing systemic activation of inflammatory, apoptotic, and antiviral pathways in response to GETV infection, indicating viral hijacking and inhibition of innate host immunity such as IFN-I/III responses. Last and most importantly, activation of the JAK-STAT signaling pathway and complement and coagulation cascades may be a primary driver for IFN-γ-mediated inhibition of GETV replication. Discussion: These findings revealed that GETV possessed the capability of viral immune escape and indicated that IFN-γ aided in the prevention and control of GETV, implying the potential molecular mechanism of suppression of GETV by IFN-γ, all of which warrant emphasis or further clarification.

分类号：

  • 相关文献

[1]The host transcriptome change involved in the inhibitory effect of exogenous interferon-& gamma; on Getah virus replication. Li, Jialei,Gao, Xintao,Liu, Xingjian,Wu, Tong,Song, Haozhi,Gao, Weisong,Jia, Hong,Li, Yinue,Zhang, Zhifang. 2023

[2]DNA Vaccine Co-Expressing Hemagglutinin and IFN-γ Provides Partial Protection to Ferrets against Lethal Challenge with Canine Distemper Virus. Jianjun Zhao,Yiyang Sun,Ping Sui,Hongjun Pan,Yijun Shi,Jie Chen,Hailing Zhang,Xiaolong Wang,Rongshan Tao,Mengjia Liu,Dongbo Sun,Jiasan Zheng. 2023

[3]Prompt Antiviral Action of Pulmonary CD8+ TRM Cells Is Mediated by Rapid IFN-γ Induction and Its Downstream ISGs in the Lung. Lang Jiang,Lu Liu,Miaomiao Zhang,Linxia Zhang,Cuisong Zhu,Qian He,Lilin Ye,Chen Zhao,Zejun Li,Jianqing Xu,Xiaoyan Zhang. 2022

[4]Differences in Pathogenicity and Vaccine Resistance Discovered between Two Epidemic Strains of Marek’s Disease Virus in China. Zheng Hao Yu,Yan Ping Zhang,Xing Ge Lan,Ya Nan Wang,Rong Rong Guo,Kai Li,Li Gao,Xiao Le Qi,Hong Yu Cui,Xiao Mei Wang,Yu Long Gao,Chang Jun Liu. 2023

[5]Correlation between 146S Antigen Content in Foot-and-Mouth Disease Inactivated Vaccines and Immunogenicity Level and Vaccine Potency Alternative Test Methods. Yongxia Li,Ruai Yang,Fu Yin,Haisheng Zhang,Guoyuan Zhai,Shiqi Sun,Bo Tian,Qiaoying Zeng. 2024

[6]检测牛IFN-γ 双抗体夹心 ELISA 的建立及在牛结核病诊断上的初步应用. 张改梅,贾红,侯绍华,鑫婷,郭晓宇,袁维峰,刘淑清,吴竞,高新桃,李明,董瑞凯,朱鸿飞. 2014

[7]自拟中药方剂对鸡传染性支气管炎的防治效果. 王萌,严作廷,叶得河,王东升,张仕栋. 2011

[8]长白猪γ-干扰素基因的原核表达与分析. 赵英杰,徐宏军,李尚波,王文成,卫广森,宣华. 2006

[9]1;25-(OH)_2D_3对体外培养围产期奶牛外周血淋巴细胞转化率及γ-干扰素(IFN-γ)的影响. 曹荣,王加启,胡菡,卜登攀,雒秋江. 2007

[10]硫酸化酵母葡聚糖对鸡淋巴细胞的影响. 王米,张丽芳,徐斌武,杨锐乐,雷娜,费陈忠,王宵旸,张可煜,薛飞群. 2016

[11]T细胞IFN-γ表达水平检测方法的建立及其在马传染性贫血疫苗免疫机理研究中的应用. 林跃智,邓喜林,沈楠,赵立平,孟庆来,马建,王君伟,邵一鸣,周建华. 2008

[12]两种MHC-B单倍型鸡群在发育过程中四种细胞因子转录水平的变化. 蔡文博,李行,武永淑,杨柳,张伟,韩凌霞. 2012

[13]猪带绦虫AgB与猪CD58或IFN-γ共表达质粒的构建及其在BHK-21细胞中的表达. 侯俊玲,景志忠,郑亚东,胡志敏,骆学农,蒙学莲,窦永喜,刘军龙,才学鹏. 2007

[14]猪IFN-γ mRNA TaqMan荧光定量RT-PCR检测方法的建立. 韦天超,田志军,周艳君,安同庆,肖燕,彭金美,姜一峰,童光志. 2009

[15]1，25-(OH)_2D_3对体外培养围产期奶牛外周血淋巴细胞转化率及γ-干扰素(IFN-γ)的影响. 曹荣,王加启,胡菡,卜登攀,雒秋江. 2007

[16]猪IFN-γ mRNA Taq Man荧光定量RT-PCR检测方法的建立. 韦天超,田志军,周艳君,安同庆,肖燕. 2009

[17]G2系和G5系鸡感染马立克氏病毒早期细胞因子转录水平的变化. 蔡文博,吴少莲,彭蔚宇,王丽廷,陈洪岩,韩凌霞. 2015

[18]检测牛IFN-γ双抗体夹心ELISA的建立及在牛结核病诊断上的初步应用. 张改梅,贾红,侯绍华,鑫婷,郭晓宇,袁维峰,刘淑清,吴竞,高新桃. 2014

[19]猪IFN-γmRNATaqMan荧光定量RT-PCR检测方法的建立. 韦天超,田志军,周艳君,安同庆,肖燕,彭金美,姜一峰,童光志. 2009

[20]细胞因子IL-17作为牛结核病诊断标志物的研究及实时荧光定量PCR检测方法的建立. 高新桃,贾红,侯绍华,郭晓宇,袁维峰,姜一曈,朱鸿飞,鑫婷. 2018

作者其他论文 更多>>