数字农科院2.0

CHOLINE TRANSPORTER-RELATED 4 (CTR4) Is Involved in Drought and Saline Tolerance in Rice

文献类型： 外文期刊

作者： Yu Shicong;Luo Ruxian;Zheng Shuqin;Ning Jing;Shi Yuanzhu;Guo Daiming;Jia Liangmeng;Wang Sen;Xiao Guizong;Guo Pengwang;Li Yang;Ma Xiaoding

作者机构：

关键词： choline transporter;drought stress;lipid homeostasis;Oryza sativa;salt stress

期刊名称： Rice Science

ISSN： 1672-6308

年卷期： 2025 年 32 卷 1 期

页码：

收录情况： SCIE（2025版） ; ; CSCD（2025-2026年度） ; ; 科技核心（2024版） ; ; 农林核心（2024版）

摘要： The tolerance of rice to drought and saline stress is crucial for maintaining yields and promoting widespread cultivation. From an ethyl methanesulfonate (EMS)-mutagenized mutant library, we identified a mutant that is susceptible to osmotic stress, named Osmotic Stress Sensitivity 1 (Oss1). Using MutMap sequencing, we characterized the role of a choline transporter-related family gene, CTR4 (Choline Transporter-Related 4), in rice's tolerance to drought and salt stress. CTR4 plays a critical role in regulating membrane lipid synthesis. In knockout mutants, the total membrane lipid content, especially unsaturated fatty acids, was significantly reduced. Compared with the wild type, knockout mutants exhibited decreased membrane lipid stability under drought and salt stress, faster water loss, higher relative electrolyte leakage, and lower levels of proline and soluble sugars, leading to impaired tolerance to drought and salt stress. In contrast, the overexpression of CTR4 enhanced seedling tolerance to drought and saline stress. The overexpression lines displayed lower malondialdehyde levels, reduced relative electrolyte leakage, and slower rates of leaf water loss under stress conditions, thereby improving seedling survival rates during stress. Moreover, lipid synthesis gene expression was down-regulated in CTR4 mutants, potentially exacerbating membrane permeability defects and further compromising stress resistance. These findings suggest that CTR4 mediates choline transport and influences cell membrane formation, thereby enhancing rice defenses against drought and salt stress by maintaining lipid homeostasis.

分类号：

  • 相关文献

[1]Changes of antioxidative enzymes and cell membrane osmosis in tomato colonized by arbuscular mycorrhizae under NaCl stress. ZhongQun, He,ChaoXing, He,ZhiBin, Zhang,ZhiRong, Zou,HuaiSong, Wang.

[2]The putative auxin efflux carrier OsPIN3t is involved in the drought stress response and drought tolerance. Qian Zhang , Jingjing Li , Wenjiao Zhang , Shuning Yan , Rui Wang , Junfeng Zhao , Yujing Li , Zhiguang Qi , Zongxiu Sun , Zhengge Zhu (2)*. 2012

[3]Genetic Dissection for Leaf Correlative Traits of Rice (Oryza sativa L.)Under Drought Stress. Guo Longbiao,Qian Qian,Zeng Dali,Dong Guojun,Teng Sheng,Zhu Lihuang. 2004

[4]Genome-Wide Analysis of the Rice PcG Gene Family and Its Involvement in Salt Response and Development. Ziang Shi,Jun Cao,Chuheng Li,Jun Liu,Xinlei Yang,Xiliu Cheng. 2025

[5]Inducible and constitutive expression of an elicitor gene Hrip1 from Alternaria tenuissima enhances stress tolerance in Arabidopsis. Qiu, De-Wen,Zeng, Hong-Mei,Guo, Li-Hua,Yang, Xiu-Fen,Liu, Zheng.

[6]Comparative proteomic analysis of alfalfa revealed new salt and drought stress-related factors involved in seed germination. Ma, Qiaoli,Kang, Junmei,Zhang, Kun,Wang, Tenghua,Sun, Yan,Kang, Junmei,Long, Ruicai,Zhang, Tiejun,Yang, Qingchuan,Ma, Qiaoli,Xiong, Junbo.

[7]Isolation and characterization of induced genes under drought stress at the flowering stage in maize (Zea mays). Li, Hui-Yong,Wang, Tian-Yu,Shi, Yun-Su,Fu, Jun-Jie,Song, Yan-Chun,Wang, Guo-Ying,Li, Yu.

[8]Simultaneous Overexpression of the HhERF2 and PeDREB2a Genes Enhanced Tolerances to Salt and Drought in Transgenic Cotton. Li, JinBo,Dong, XueNi,Shao, JiRong,Li, JinBo,Dong, XueNi,Lei, Zhi,Li, YongLiang,Yang, PeiYang,Tao, Fei,Wu, YanMin,Zhao, Liang,Li, Shi-Gang,Du, LinFeng.

[9]Molecular mechanism that underlies cotton response to salt and drought stress revealed by complementary transcriptomic and iTRAQ analyses. Zeze Yuan,Chaohui Zhang,Weidong Zhu,Gentu Yan,Xiugui Chen,Ping Qiu,Boymurodov Ruzimurod,Wuwei Ye,Bobokhonova Zebinisso Qaraevna,Zujun Yin. 2023

[10]Genome-Wide Investigation of the NAC Transcription Factor Family in Apocynum venetum Revealed Their Synergistic Roles in Abiotic Stress Response and Trehalose Metabolism. Huang, Xiaoyu,Qiu, Xiaojun,Wang, Yue,Abubakar, Aminu Shehu,Chen, Ping,Chen, Jikang,Chen, Kunmei,Yu, Chunming,Wang, Xiaofei,Gao, Gang,Zhu, Aiguo. 2023

[11]Genome wide identification and characterization of MAPK genes reveals their potential in enhancing drought and salt stress tolerance in Gossypium hirsutum. Sadau, Salisu Bello,Mehari, Teame Gereziher,Ahmad, Adeel,Tajo, Sani Muhammad,Ibrahim, Sani,Iqbal, Muhammad Shahid,Elasad, Mohammed,Zhang Jingjing,Wei Hengling,Yu Shuxun. 2022

[12]Overexpression of lncRNA77580 Regulates Drought and Salinity Stress Responses in Soybean. Xiangqian Chen,Xuemin Jiang,Fengjuan Niu,Xianjun Sun,Zheng Hu,Fei Gao,Hui Zhang,Qiyan Jiang. 2023

[13]Na+ AND K+ HOMEOSTASIS IS IMPORTANT FOR SALINITY AND DROUGHT TOLERANCE OF CALLIGONUM MONGOLICUM. Hu, Jing,Hu, Xiaoke,Duan, Huirong,Zhang, Huiwen,Yu, Qiushi. 2021

[14]Small secreted peptides encoded on the wheat (triticum aestivum L.) genome and their potential roles in stress responses. Tian D.,Xie Q.,Deng Z.,Xue J.,Li W.,Zhang Z.,Dai Y.,Zheng B.,Lu T.,De Smet I.,Guo Y.. 2022

[15]Systematic analysis of Histidine photosphoto transfer gene family in cotton and functional characterization in response to salt and around tolerance. Lanjie Zhao,Liangqing Sun,Lixue Guo,Xuke Lu,Waqar Afzal Malik,Xiugui Chen,Delong Wang,Junjuan Wang,Shuai Wang,Chao Chen,Taili Nie,Wuwei Ye. 2022

[16]The Dendrobium catenatum DcCIPK24 increases drought and salt tolerance of transgenic Arabidopsis. Tingting Zhang,Yuxin Li,Yuqian Kang,Peng Wang,Wei Li,Wengang Yu,Jian Wang,Xiqiang Song,Xingyu Jiang,Yang Zhou. 2022

[17]Genome-wide identification of CBL family genes in Nicotiana tabacum and the functional analysis of NtCBL4A-1 under salt stress. Jingjing Mao,Guang Yuan,Kaiyan Han,Haiying Xiang,Wanli Zeng,Richard G.F. Visser,Yuling Bai,C. Gerard van der Linden,Haobao Liu,Qian Wang. 2023

[18]GhMAPK3, a mitogen-activated protein kinase, enhance salt and drought tolerance in cotton (Gossypium hirsutum). Hongliang Jian,Salisu Bello Sadau,Fei Wei,Adeel Ahmad,Zhengying Lu,Liang Ma,Xiaokang Fu,Nan Zhang,Jianhua Lu,Guo Yin,Hantao Wang,Hengling Wei. 2024

[19]BrERF109 positively regulates the tolerances of drought and salt stress in Chinese cabbage. Chao Li,Jian Cui,Yongrui Shen,Qi Zeng,Ruixing Zhang,Xue Bai,Qingguo Sun,Xiaowu Wang,Lin Chen,Baohua Li. 2024

[20]Genome-Wide Identification and Expression Profile Analysis of the NADPH Oxidase Gene Family in Avena sativa L.. Jiang, Qingxue,Zhou, Xinyue,Tang, Jun,Yi, Dengxia,Ma, Lin,Wang, Xuemin. 2025

作者其他论文 更多>>