数字农科院2.0

Multi-omics analyses reveal systemic insights into maize vivipary

文献类型: 外文期刊

作者: Yiru Wang;Junli Zhang;Minghao Sun;Cheng He;Ke Yu;Bing Zhao;Rui Li;Jian Li;Zongying Yang;Xiao Wang;Haiyang Duan;Junjie Fu;Sanzhen Liu;Xuebin Zhang;Jun Zheng

作者机构:

关键词: Abscisic acid;Germination;Maize;Seed dormancy;Vivipary

期刊名称: Plants

ISSN: 2223-7747

年卷期: 2022 年 10.0 卷 11.0 期

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摘要: Maize vivipary, precocious seed germination on the ear, affects yield and seed quality. The application of multi-omics approaches, such as transcriptomics or metabolomics, to classic vivipary mutants can potentially reveal the underlying mechanism. Seven maize vivipary mutants were selected for transcriptomic and metabolomic analyses. A suite of transporters and transcription factors were found to be upregulated in all mutants, indicating that their functions are required during seed germination. Moreover, vivipary mutants exhibited a uniform expression pattern of genes related to abscisic acid (ABA) biosynthesis, gibberellin (GA) biosynthesis, and ABA core signaling. NCED4 (Zm00001d007876), which is involved in ABA biosynthesis, was markedly downregulated and GA3ox (Zm00001d039634) was upregulated in all vivipary mutants, indicating antagonism between these two phytohormones. The ABA core signaling components (PYL-ABI1-SnRK2-ABI3) were affected in most of the mutants, but the expression of these genes was not significantly different between the vp8 mutant and wild-type seeds. Metabolomics analysis integrated with co-expression network analysis identified unique metabolites, their corresponding pathways, and the gene networks affected by each individual mutation. Collectively, our multi-omics analyses characterized the transcriptional and metabolic landscape during vivipary, providing a valuable resource for improving seed quality.

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