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Multiomics insights into the TNT degradation mechanism by Pantoea sp. BJ2 isolated from an ammunition destruction site

文献类型： 外文期刊

作者： You Li;Junpeng Luo;Xiaoyong Liao;Hongying Cao;Junting Pan;Anina James;Haonan Li

作者机构：

关键词： Biodegradation;Metabolic pathways;Multiomics analysis;Pantoea sp.;Trinitrotoluene

期刊名称： Chemical Engineering Journal

ISSN： 1385-8947

年卷期： 2024 年 497 卷

页码：

收录情况： SCIE（2024版） ; ; EI（2024版）

摘要： Trinitrotoluene (TNT) is a highly toxic organic pollutant, and its biodegradation process and mechanism remain unclear. In this study, five TNT-degrading bacteria were isolated and identified, among which Pantoea sp. BJ2 showed the best TNT degradation potential; it could degrade 97.85 % of 100 mg L−1 TNT within 24 h. Further, five genes (three nemA, one nfsA and one nfnB) directly related to TNT metabolism were identified in Pantoea sp. BJ2. During the degradation of TNT by Pantoea sp. BJ2, N-ethylmaleimide reductase, nitrate reductase and ATP-binding protein of the nitrate/nitrite transport system were found to be significantly upregulated, and five metabolites, including 2-amino-4,6-dinitrotoluene, were also identified. Based on the multiomics analysis, the mechanism of TNT degradation by Pantoea sp. BJ2 was proposed, indicating that the degradation of TNT by Pantoea sp. BJ2 was achieved under the joint action of various functional genes and proteins, including material transport, energy supply and exogenous material metabolism. The findings offer reliable theoretical underpinnings and technical support for a safe remediation of TNT-contaminated sites, providing new insights into the microbial degradation mechanisms of TNT from a multiomics perspective.

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