数字农科院2.0

Genome-wide identification of cytochrome c oxidase genes in cotton and functional characterization of GhCOX11 in drought and cold stress

文献类型： 外文期刊

作者： Chenhui Li;Xingzhi Wei;Qihua Chen;Xinyue Xu;Weijiang Tian;Xiumei Dai;Xiaofeng Su;Wenfang Guo;Hongmei Cheng;Huan Si;Peilin Wang;Huiming Guo

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关键词： Cotton;Genome-wide identification;GhCOX11;Glutathione peroxidase (GPX);Reactive oxygen species (ROS)

期刊名称： International Journal of Biological Macromolecules

ISSN： 0141-8130

年卷期： 2025 年 329 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Abiotic stresses significantly impair crop morphogenesis, physiological function, metabolic networks, and yield. Moreover, multiple stress factors often act synergistically, highlighting the urgent need to breed multi-stress-tolerant, high-quality cotton varieties. In this study, the cytochrome C oxidase (COX) gene family was identified genome-wide in Gossypium hirsutum using a Hidden Markov Model (HMM), revealing 50 members categorized into 12 subfamilies. Chromosomal mapping revealed an uneven distribution across 22 chromosomes, with evidence of both intra- and interspecific collinearity. Further functional analysis focused on GhCOX11, which was broadly expressed across tissues and significantly upregulated under drought, salt, and low-temperature stresses. Silencing GhCOX11 led to increased leaf wilting, reduced relative water content, and elevated hydrogen peroxide (H2O2) accumulation under drought stress. Conversely, overexpressing GhCOX11 in tobacco significantly enhanced drought and cold tolerance, as evidenced by improved root development and increased antioxidant enzyme activity. The transcription factor DREB1D was found to specifically bind the low-temperature response element (LTR) in the GhCOX11 promoter, activating its expression. Additionally, GhCOX11 interacted with members of the glutathione peroxidase (GPX) family to synergistically scavenge reactive oxygen species (ROS), thereby maintaining cellular redox homeostasis. These findings clarify the role of GhCOX11 in plant stress responses through ROS regulation and offer promising gene candidates for improving crop stress resilience.

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