数字农科院2.0

Brucella abortus Rough-Type Mutant Induces Ferroptosis and More Oxidative Stress in Infected Macrophages

文献类型： 外文期刊

作者： Hai Hu;Guangdong Zhang;Mingxing Tian;Xiang Guan;Yi Yin;Chan Ding;Shengqing Yu

作者机构：

关键词： Brucella;ferroptosis;macrophage;oxidative stress

期刊名称： Pathogens

ISSN： 2076-0817

年卷期： 2023 年 12 卷 10 期

页码：

收录情况： SCIE（2023版）

摘要： Brucella is an intracellular parasitic bacterium that uses multiple strategies to evade the host’s defense mechanisms. However, how Brucella manipulates the host-induced oxidative stress and relevant biological processes are still poorly understood. In this study, a comparative transcriptome assay of macrophages infected with Brucella abortus S2308 and its rough mutant RB14 was performed to investigate the differentially expressed genes which might be associated with the pathogenic mechanism of Brucella. Our results showed that numerous host pro-oxidative and antioxidative stress genes were differentially expressed in macrophages infected with B. abortus S2308 and mutant RB14 at 4, 8, 24, and 48 h post-infection. Interestingly, we found that several ferroptosis-associated genes were differentially expressed during B. abortus RB14 infection. Moreover, we found that the rough mutant RB14-induced macrophage death was associated with reduced levels of host glutathione and glutathione peroxidase 4, together with increased free iron, lipid peroxidation, and ROS, all of which are important hallmarks of ferroptosis. The ferroptosis occurring during infection with RB14 was reduced by treatment with the inhibitor ferrostatin-1. However, B. abortus S2308 infection did not induce these hallmarks of ferroptosis. Taken together, our results demonstrate that ferroptosis is involved in rough B. abortus infection. Investigating how Brucella manipulates oxidative stress and ferroptosis in its host will be helpful to clarify the pathogenicity of B. abortus.

分类号：

  • 相关文献

[1]Brucella rough RB51 infection activates P53-Slc7a11-Gpx4/GSH pathway to induce ferroptosis to attenuate the intracellular survival on macrophages. Hai Hu,Guangdong Zhang,Mingxing Tian,Yi Yin,Yanqing Bao,Xiang Guan,Chan Ding,Shengqing Yu. 2024

[2]Brucella Manipulates Host Cell Ferroptosis to Facilitate Its Intracellular Replication and Egress in RAW264.7 Macrophages. Guangdong Zhang,Hai Hu,Yi Yin,Mingxing Tian,Zhigao Bu,Chan Ding,Shengqing Yu. 2024

[3]S100A4 regulates ferroptosis to suppress cell proliferation via the SLC7A11-GSH axis but facilitates Brucella infection in RAW264.7 macrophages. Guangdong Zhang,Yi Yin,Hai Hu,Mingxing Tian,Chan Ding,Shengqing Yu. 2025

[4]Transcriptomic analysis revealed ferroptosis in ducklings with splenic necrosis induced by NDRV infection. Wang, Hongzhi,Jiang, Chenchen,Xu, Boyi,Lei, Di,Fang, Rendong,Tang, Yi. 2025

[5]Novel Duck Orthoreovirus Induces Ferroptosis in HD11 Cells by Hijacking Cellular Iron Metabolism and Promoting Iron Accumulation. Hongzhi Wang,Di Lei,Chenchen Jiang,Boyi Xu,Yi Tang,Rendong Fang. 2026

[6]Quercetin Reprograms Immunometabolism of Macrophages via the SIRT1/PGC-1α Signaling Pathway to Ameliorate Lipopolysaccharide-Induced Oxidative Damage. Jing Peng,Zhen Yang,Hao Li,Baocheng Hao,Dongan Cui,Ruofeng Shang,Yanan Lv,Yu Liu,Wanxia Pu,Hongjuan Zhang,Jiongjie He,Xuehong Wang,Shengyi Wang. 2023

[7]Combined ecotoxicological effects of different-sized polyethylene microplastics and imidacloprid on the earthworms (Eisenia fetida). Fu H.,Zhu L.,Mao L.,Zhang L.,Zhang Y.,Chang Y.,Liu X.,Jiang H.. 2023

[8]Phospholipid Phosphatase 3 (PLPP3) Induces Oxidative Stress to Accelerate Ovarian Aging in Pigs. Hongyan Quan,Yixuan Guo,Shuo Li,Yao Jiang,Qingpeng Shen,Yingting He,Xiaofeng Zhou,Xiaolong Yuan,Jiaqi Li. 2024

[9]MNQ derivative D19 alleviates LPS-induced inflammation and oxidative stress in sheep follicular granulosa cells through the GPX4-mediated ferroptosis. Chen, Chunlu,Lu, Jia,Qu, Yuhan,Dong, Jianhua,Hong, Yihao,Ren, Yongping,Jiang, Shouqing,Baptista, Rafael,Wang, Dong,Mur, Luis Aj,Lyu, Lihua. 2025

[10]Plant sterol ester of α-linolenic acid protects against ferroptosis in metabolic dysfunction-associated fatty liver disease via activating the Nrf2 signaling pathway. Hao Han,Liyuan Pei,Yajing Dong,Yanyang Han,Shuqi Ji,Xiaoman Wang,Mingming Zheng. 2025

[11]Aspirin Eugenol Ester Alleviates Vascular Endothelial Ferroptosis by Enhancing Antioxidant Ability and Inhibiting the JNK/c-Jun/NCOA4/FTH Signaling Pathway. Feng, Ji,Tao, Qi,Zhang, Zhi-Jie,Yu, Qin-Fang,Yang, Ya-Jun,Li, Jian-Yong. 2025

[12]EIAV疫苗弱毒株诱导的超感染抵制现象与其诱导天然免疫激活间的相关性研究. 马建,王珊珊,林跃智,王雪峰,杜承,周建华. 2012

[13]堆形艾美耳球虫巨噬细胞移动抑制因子克隆和表达. 阎晓菲,韩红玉,黄兵,岳城,赵其平,姜连连,董辉,卞庆松,张建哲. 2009

[14]Inflammatory Mechanism of Brucella Infection in Placental Trophoblast Cells. Xiao Y.,Li M.,Guo X.,Zeng H.,Shuai X.,Guo J.,Huang Q.,Chu Y.,Zhou B.,Wen J.,Liu J.,Jiao H.. 2022

[15]Lable-Free Based Comparative Proteomic Analysis O.f Secretory Proteins Of R ough Brucella Mutants. Li, P, Tian, MX, Hu, H, Yin, Y, Guan, X, Ding, C, Wang, SH, Yu, SQ. 2019

[16]Molecular Epidemiological Characterization Of Brucella I.solates From Sheep And Y aks In Northwest China. Cao, X, Li, Z, Liu, Z, Fu, B, Liu, Y, Shang, Y, Zhou, J, Jing, Z. 2018

[17]An extracytoplasmic function sigma/anti-sigma factor system regulates hypochlorous acid resistance and impacts expression of the type IV secretion system in brucella melitensis. Huoming Li,Sen Hu,Xin Yan,Yan Yang,Wenxing Liu,Zhigao Bu,Ganwu Li,Wentong Cai. 2021

[18]Brucella Infection Regulates Peroxiredoxin-5 Protein E.xpression To Facilitate Intracellular S urvival By Reducing The Production Of Nitric Oxide And Reactive Oxygen Species. Hu, H, Tian, MX, Li, P, Bao, YQ, Guan, X, Lian, ZM, Yin, Y, Ding, C, Yu, SQ. 2019

[19]Brucella Rough Mutant Induce Macrophage D.eath Via Activating Ire1 A lpha Pathway Of Endoplasmic Reticulum Stress By Enhanced T4Ss Secretion. Li, P, Tian, MX, Bao, YQ, Hu, H, Liu, JM, Yin, Y, Ding, C, Wang, SH, Yu, SQ. 2017

[20]Tracing Brucella evolutionary dynamics in expanding host ranges through nucleotide, codon and amino acid usages in genomes. Jian hua Zhou,Hua Li,Xuerui Li,Jing Gao,Long Xu,Shengyi Han,Yongsheng Liu,Youjun Shang,Xiaoan Cao. 2021

作者其他论文 更多>>