数字农科院2.0

Viral metagenomic analysis of fecal samples from Bos grunniens on the Qinghai-Tibet Plateau reveals novel picornaviruses and diverse CRESS-DNA viruses

文献类型： 外文期刊

作者： Jiaheng Chen;Ga Gong;Xiaodong Su;Xiaofei Song;Jingyue Zhang;Ping Wu;Hua Wang;Tongling Shan;Wen Zhang

作者机构：

关键词： CRESS-DNA viruses;fecal virome;metagenomics;Picornaviridae;Qinghai-Tibet Plateau;yak

期刊名称： Frontiers in Cellular and Infection Microbiology

ISSN：

年卷期： 2026 年 15 卷

页码：

收录情况： SCIE（2025版）

摘要： Introduction: The Qinghai–Tibet Plateau (QTP), one of the most extreme environments on Earth, provides a unique natural setting for exploring viral diversity and evolution under conditions of high altitude, hypoxia, and intense ultraviolet radiation. The yak (Bos grunniens), a key endemic ruminant species of the QTP, plays an essential ecological and economic role, yet its fecal virome remains poorly characterized. Methods: In this study, we analyzed 43 yak fecal samples collected from Yushu, Qinghai Province, and constructed nine metagenomic libraries to investigate the composition, diversity, and phylogenetic characteristics of the yak fecal virome. Results: Metagenomic sequencing generated approximately 463 million raw reads, of which 2.87 million were classified as viral. The viral reads in the sequenced libraries were primarily composed of single-stranded DNA viruses (92.46%), particularly members of Smacoviridae, Circoviridae, and Genomoviridae, whereas RNA viruses such as Picornaviridae accounted for a minor fraction (0.71%). Phylogenetic analyses revealed that several circular single-stranded DNA (CRESS-DNA) virus and picornavirus genomes share high similarity with known ruminant-associated viruses, while forming independent evolutionary clades, suggesting potential cross-species transmission among plateau animals. The large-scale divergence within Smacoviridae further reflects extensive lineage expansion under the plateau’s extreme environmental pressures. Discussion: Compared with our previous yak virome study, this work provides independent and complementary insights into the genomic and evolutionary characteristics of key viral taxa. Overall, our findings expand the genomic landscape of the yak fecal virome and highlight the Qinghai–Tibet Plateau as an important reservoir for exploring viral diversity, evolution, and host–environment interactions in extreme ecosystems.

分类号：

  • 相关文献

[1]Comparative analysis of gut fungal composition and structure of the yaks under different feeding models. Zhu, Yanbin,Cidan, Yangji,Sun, Guangming,Li, Xin,Shahid, Muhammad Akbar,Luosang, Zhaxi,Suolang, Zhaxi,Suo, Lang,Basang, Wangdui. 2023

[2]Altered microbiota, antimicrobial resistance genes, and functional enzyme profiles in the rumen of yak calves fed with milk replacer. Zhuang, Yimin,Guo, Wei,Cui, Kai,Tu, Yan,Diao, Qiyu,Zhang, Naifeng,Bi, Yanliang,Ma, Tao. 2023

[3]“大通”牦牛Lfcin基因克隆及生物信息学分析(英文). 裴杰,阎萍,姬国红,冯瑞林,梁春年,郭宪,曾玉峰,包鹏甲,褚敏. 2009

[4]Advances and Breakthroughs in IRES-Directed Translation and Replication of Picornaviruses. Abdullah, Sahibzada Waheed,Wu, Jin'en,Wang, Xuefei,Guo, Huichen,Sun, Shiqi. 2023

[5]An anti-picornaviral strategy based on the crystal structure of foot-and-mouth disease virus 2C protein. Chu Zhang,Fan Yang,Justyna Aleksandra Wojdyla,Bo Qin,Wei Zhang,Min Zheng,Weijun Cao,Meitian Wang,Xiaopan Gao,Haixue Zheng,Sheng Cui. 2022

[6]Type I Interferon Induced And A.ntagonized By Foot-And-Mouth Disease V irus. Ma, XX,Ma, LN,Chang, QY,Ma, P,Li, LJ,Wang, YY,Ma, ZR,Cao, X. 2018

[7]Retrieval snow depth by artificial neural network methodology from integrated AMSR-E and in-situ data-A case study in Qinghai-Tibet Plateau. Cao Yungang,Yang Xiuchun,Zhu Xiaohua. 2008

[8]Genetic characterization of Toxoplasma gondii from Qinghai vole, Plateau pika and Tibetan ground-tit on the Qinghai-Tibet Plateau, China. Zhang, Xiao-Xuan,Lou, Zhong-Zi,Huang, Si-Yang,Zhou, Dong-Hui,Jia, Wan-Zhong,Su, Chunlei,Zhu, Xing-Quan,Zhang, Xiao-Xuan,Su, Chunlei,Zhu, Xing-Quan. 2013

[9]Characterization of symbiotic and endophytic bacteria isolated from root nodules of herbaceous legumes grown in Qinghai-Tibet plateau and in other zones of China. Chen, Zhong Yi,Wang, En Tao,Tian, Chang Fu,Sui, Xin Hua,Chen, Wen Xin. 2007

[10]Sensitivity of Grassland Coverage to Climate across Environmental Gradients on the Qinghai-Tibet Plateau. Rihan Wu,Guozheng Hu,Hasbagan Ganjurjav,Qingzhu Gao. 2023

[11]Mapping of lakes in the Qinghai-Tibet Plateau from 2016 to 2021: trend and potential regularity. Zhichong Yang,Si Bo Duan,Xiaoai Dai,Yingwei Sun,Meng Liu. 2022

[12]Regional distribution and migration potential of antibiotic resistance genes in croplands of Qinghai Tibet Plateau. Dagang Song,Xue Tang,Akash Tariq,Kaiwen Pan,Dong Li. 2023

[13]Morphometrical analyses revealed high diversity of the eastern honey bee (Apis cerana) in mountains and islands in China. Ji, Congcong,Shi, Wei,Tang, Jiao,Ji, Ting,Gao, Jinglin,Liu, Feng,Shan, Jinqiong,Chen, Xiao,Chen, Chao. 2023

[14]Gut Virome of the World's Highest-Elevation Lizard Species (Phrynocephalus erythrurus and Phrynocephalus theobaldi) Reveals Versatile Commensal Viruses. Juan Lu,Shixing Yang,Chunmei Wang,Hao Wang,Ga Gong,Yuan Xi,Jiamin Pan,Xiaochun Wang,Jian Zeng,Ju Zhang,Peng Li,Quan Shen,Tongling Shan,Wen Zhang. 2022

[15]Expansion of Cyclophyllidea Biodiversity in Rodents of Qinghai-Tibet Plateau and the “Out of Qinghai-Tibet Plateau” Hypothesis of Cyclophyllideans. Yao Dong Wu,Guo Dong Dai,Li Li,D. Timothy J. Littlewood,John Asekhaen Ohiolei,Lin Sheng Zhang,Ai Min Guo,Yan Tao Wu,Xing Wei Ni,Nigus Abebe Shumuye,Wen Hui Li,Nian Zhang Zhang,Bao Quan Fu,Yong Fu,Hong Bin Yan,Wan Zhong Jia. 2022

[16]Reclamation intensifies the positive effects of warming on N2O emission in an alpine meadow. Zheng Li,Yan Li,Guozheng Hu,Hongbao Wu,Yan Liang,Jun Yan,Shicheng He,Hasbagan Ganjurjav,Qingzhu Gao. 2023

[17]Rest-grazing before soil thawing alters the below-ground distribution and extent of rooting in Carex subalpine meadows. Jing, Yuanyuan,Xiao, Hong,Xu, Changlin,Wang, Lin,Chen, Yanzhu,Liu, Yuanyuan,Yu, Xiaojun. 2023

[18]Spatial and temporal dynamics of livestock grazing intensity in the Selinco region: Towards sustainable grassland management. Guilin Xi,Changhui Ma,Fangkun Ji,Hongxin Huang,Haoyan Zhang,Zecheng Guo,Xueyuan Zhang,Sha Zhao,Yaowen Xie. 2024

[19]Spatio-Temporal Dynamics of Vegetation and Its Driving Mechanisms on the Qinghai-Tibet Plateau from 2000 to 2020. Changhui Ma,Si Bo Duan,Wenhua Qin,Feng Wang,Lei He. 2024

[20]Genomic vulnerability and local adaptation of an arid tolerant tree species on the Qinghai-Tibet Plateau. Tang, Jieshi,Ruhsam, Markus,Feng, Shuo,Milne, Richard,Yang, Heng,Kuang, Jingge,Tao, Wenjing,Wang, Yi,Li, Jialiang,Bakhtiyorov, Zulfiyor,Oimahmad, Rahmonov,Mao, Kangshan. 2025

作者其他论文 更多>>