数字农科院2.0

Genomic diversity at the Glu-D1 locus in Aegilops tauschii reveals the origin of elite high-molecular-weight glutenin genes in bread wheat (Triticum aestivum)

文献类型: 外文期刊

作者: 徐婉欣;耿帅锋;刘少帅;Andrea Gonzalez-Munoz;刘一凡;邓中印;车育青;崔达达;邹新宇;王梓颖;王翔;王道文;刘冬成;周云;刘登才;李爱丽;毛龙

作者机构:

关键词: 高分子量麦谷蛋白;;山羊草;;籽粒品质;;Dx5与Dy10

期刊名称: Plant Diversity

ISSN: 2096-2703

年卷期: 2025 年

页码:

收录情况: SCIE(2025版) ; ; CSCD(2025-2026年度) ; ; 科技核心(2024版)

摘要: 节节麦(Aegilops tauschii)基因组的Glu-D1位点携带两种高分子量麦谷蛋白基因,即Dx和Dy基因,这两种基因对面包小麦面团的黏弹性至关重要,有助于面包小麦成为全球主要粮食作物。本研究对48份节节麦高质量基因组组装序列以及一个庞大的Glu-D1位点数据集(该位点因其基因组复杂性而难以进行高分辨率分析)进行了研究。系统发育分析支持存在六个主要分支,尽管与基于地理分布的分类略有差异。尽管基因组距离较短,但基于基因的单倍型分析检测到Dx和Dy基因之间可能存在祖先重组,这两个基因被独特的重复序列分隔开来。从生化角度来看,长度相同的麦谷蛋白其等电点可能存在差异,导致其在传统十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)凝胶上的迁移情况出现偏差。在不同分支之间以及Dx和Dy麦谷蛋白基因之间检测到了不同的选择压力。两个分支,即L2E-1和L2W-2,其乳糜泻致病基序相对较低,被认为最有可能是面包小麦的祖先供体。此外,关键氨基酸被认为适合进行单碱基编辑以创造新型优良等位基因。剖析Glu-D1位点的基因组多样性,有助于我们深入了解这些长期研究的种子贮藏蛋白的进化轨迹,并为改善小麦籽粒品质提供新策略。

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